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Sperm cryopreservation is an important tool for reproductive management; however, it has not been possible to perform this procedure for coho salmon. This study was designed to assess the effect of cryopreservation on sperm functional parameters, oxidative stress markers and fertilizing capacity ...


Sandoval Vargas, LeydyDumorne, KellyContreras, PabloFarias, Jorge G.Figueroa Villalobos, ElíasRisopatron, JennieValdebenito Isler, Iván[Cryopreservation of coho salmon sperm (Oncorhynchus kisutch): Effect on sperm function, oxidative stress and fertilizing capacity]Cryopreservation of coho salmon sperm (Oncorhynchus kisutch): Effect on sperm function, oxidative stress and fertilizing capacity

Patagonian blenny (E. maclovinus) is a marine species recently placed in captivity and which are potentially farmable. Understanding and improving its sperm capacity to withstand short-term storage conditions is a key element of initiating an artificial propagation program for this species. The a...


Ulloa Rodriguez, PatricioContreras, PabloDumorne, KellyLee Estevez, ManuelDiaz, RommyFigueroa Villalobos, ElíasValdebenito Isler, IvánRisopatron, JennieFarias, Jorge G.[Patagonian blenny (Eleginops maclovinus) spermatozoa quality after storage at 4 degrees C in Cortland medium]Patagonian blenny (Eleginops maclovinus) spermatozoa quality after storage at 4 degrees C in Cortland medium

The main objective of this work was to study the effects of banana flour as energy sources on broiler performance. Seventy-five broilers were randomly distributed into five groups each with 15 broilers (n = 15 broilers/group). The broilers were grouped to maize-soybean meal diet as control, T-1: ...


Dumorne, KellyAstorga-Elo, MarciaMerino, OsvaldoSevere, RolordMorante, Laura[Importance of banana flour and its effect on growth performance of broiler]Importance of banana flour and its effect on growth performance of broiler

In this study, the morphology and ultrastructure of Eleginops maclovinus spermatozoa were characterized through scanning and electron microscopy. Findings revealed that E. maclovinus spermatozoa can be differentiated into three major parts: a spherical head without acrosome (typical for externall...


Ulloa Rodriguez, PatricioContreras, PabloDumorne, KellyFigueroa Villalobos, ElíasRisopatron, JennieValdebenito Isler, IvánFarias, Jorge G.[Sperm morphology and ultrastructure of Patagonian blenny (Eleginops maclovinus)]Sperm morphology and ultrastructure of Patagonian blenny (Eleginops maclovinus)

Sperm motility in fish with external fertilization is critical for reproductive efficiency in aquaculture, especially in salmonids. Gamete preservation techniques, such as cryopreservation, however, reduce sperm motility and fertilizing capacity. Very few studies have addressed cryodamage from en...


Lee Estevez, ManuelHerrera, LisandraDiaz, RommyBeltran, JorgeFigueroa Villalobos, ElíasDumorne, KellyUlloa Rodriguez, PatricioShort, StefaniaRisopatron, JennieValdebenito Isler, IvánFarias, Jorge[Effects of cryopreservation on cAMP-dependent protein kinase and AMP-activated protein kinase in Atlantic salmon (Salmo salar) spermatozoa: Relation with post-thaw motility]Effects of cryopreservation on cAMP-dependent protein kinase and AMP-activated protein kinase in Atlantic salmon (Salmo salar) spermatozoa: Relation with post-thaw motility

The aim of this study was to evaluate effects of selection using the Percoll density gradient method on motility, mitochondrial membrane potential (Delta Psi Mit) and fertility in a subpopulation of testicular spermatozoa obtained from Atlantic salmon (Salmo salar). Samples were divided into thre...


Bravo, WilliamsDumorne, KellyBeltran Lissabet, JorgeJara Seguel, PedroRomero, JaimeFarias, Jorge G.Risopatron, JennieValdebenito Isler, IvánFigueroa Villalobos, Elías[Effects of selection by the Percoll density gradient method on motility, mitochondrial membrane potential and fertility in a subpopulation of Atlantic salmon (Salmo salar) testicular spermatozoa]Effects of selection by the Percoll density gradient method on motility, mitochondrial membrane potential and fertility in a subpopulation of Atlantic salmon (Salmo salar) testicular spermatozoa

Short-term storage of semen is a necessary key procedure in fish; it allows maximizing the use of gametes. Nevertheless, sperm quality decreases during storage has been associated with oxidative stress damage due to an increase in reactive oxygen species (ROS) during storage. This study was desig...


Merino, OsvaldoDumorne, KellySandoval Vargas, LeydyFigueroa Villalobos, ElíasValdebenito Isler, IvánFarias, Jorge G.Risopatron, Jennie[Short-term storage sperm of coho salmon (Oncorhynchus kisutch) at 4 degrees C: Effect of sperm: Extender dilution ratios and antioxidant butyl-hydroxytoluene (BHT) on sperm function]Short-term storage sperm of coho salmon (Oncorhynchus kisutch) at 4 degrees C: Effect of sperm: Extender dilution ratios and antioxidant butyl-hydroxytoluene (BHT) on sperm function

The cold storage and cryopreservation of semen decrease sperm quality. Morphological and biochemical analyses of spermatozoa provide valuable information for the optimization of storage protocols to obtain a sufficient number of spermatozoa for in vitro fertilization. The aim of this study was to...


Diaz, RommyLee-Estevez, ManuelQuinones, JohnDumorne, KellyShort, StefaniaUlloa-Rodriguez, PatricioValdebenito Isler, IvánSepulveda, NestorFarias, Jorge G.[Changes in Atlantic salmon (Salmo salar) sperm morphology and membrane lipid composition related to cold storage and cryopreservation]Changes in Atlantic salmon (Salmo salar) sperm morphology and membrane lipid composition related to cold storage and cryopreservation

Ca2+ is a key element in the sperm activation process of Salmo salar. However, the molecular mechanisms by which this ion enters the sperm cell have been poorly studied. In this study, we examined, for the first time, the role of the voltage-gated T-type Ca2+ channel in the activation of sperm mo...


Beltran, Jorge F.Herrera Belen, LisandraLee Estevez, ManuelFigueroa Villalobos, ElíasDumorne, KellyFarias, Jorge G.[The voltage-gated T-type Ca2+ channel is key to the sperm motility of Atlantic salmon (Salmo salar)]The voltage-gated T-type Ca2+ channel is key to the sperm motility of Atlantic salmon (Salmo salar)

In this study, the morphology and ultrastructure of Genypterus blacodes spermatozoa were characterized through scanning and transmission electron microscopy. Findings revealed that the G. blacodes spermatozoa can be differentiated into three major parts: a spherical head without an acrosome (typi...


Dumorne, KellyValdebenito Isler, IvánRisopatron, JennieFigueroa Villalobos, ElíasDiaz, RommyFarias, Jorge[Morphology and ultrastructure of pink cusk-eel (Genypterus blacodes, Schneider 1801) spermatozoa by scanning and transmission electron microscopy]Morphology and ultrastructure of pink cusk-eel (Genypterus blacodes, Schneider 1801) spermatozoa by scanning and transmission electron microscopy

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