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The genetic diversity of 76 accessions of lima bean (Phaseolus lunatus L.), collected mostly from home gardens, was assessed with AFLPs and seed descriptors to evaluate the potential for recovering a lost ex situ collection in Cuba. The sample contained 60 accessions collected from 25 home garden...


Castineiras Alfonso, LGuzmán, F.A.Duque E., Myriam CristinaShagarodsky, TCristobal, RVicente, M.C. de[AFLPs and morphological diversity of Phaseolus lunatus L. in Cuban home gardens : Approaches to recovering the lost ex situ collection]AFLPs and morphological diversity of Phaseolus lunatus L. in Cuban home gardens : Approaches to recovering the lost ex situ collection

Summary: Xanthomonas axonopodis pv. manihotis (Xam) is the causative agent of cassava bacterial blight (CBB), a worldwide disease that is particularly destructive in South America and Africa. CBB is controlled essentially through the use of resistant varieties. To develop an appropriate disease m...


Restrepo, S.Duque E., Myriam CristinaTohme, Joseph M.Verdier, Valérie[AFLP fingerprinting : An efficient technique for detecting genetic variation of Xanthomonas axonopodis pv. manihotis]AFLP fingerprinting : An efficient technique for detecting genetic variation of Xanthomonas axonopodis pv. manihotis

Despite the worldwide importance of cultivated cassava (M. esculenta Crantz) its origin and taxonomic relationships with other species in the genus have not been clearly established. We evaluated a representative sample of the crop’s diversity and six wild taxa with AFLPs to estimate genetic rela...


Roa, ACMaya, M.M.Duque E., Myriam CristinaTohme, Joseph M.Allem, ACBonierbale, Merideth W.[AFLP analysis of relationships among cassava and other Manihot species]AFLP analysis of relationships among cassava and other Manihot species

Cassava bacterial blight (CBB) is caused by Xanthomonas axonopodis pv. manihotis (Xam). Resistance is found in Manihot esculenta and, in addition, has been introgressed from a wild relative, M. glaziovii. The resistance is thought to be polygenic and additively inherited. Ninety-three varieties o...


Sánchez, G.M.Restrepo, S.Duque E., Myriam CristinaFregene, Martin A.Bonierbale, Merideth W.Verdier, Valérie[AFLP assessment of genetic variability in cassava accessions (Manihot esculenta) resistant and susceptible to the cassava bacterial blight (CBB)]AFLP assessment of genetic variability in cassava accessions (Manihot esculenta) resistant and susceptible to the cassava bacterial blight (CBB)

Using AFLPs, 71 peppers (Capsicum) accessions from the species C. chinense Jacq., C. baccatum L., C. annuum L. and C. frutescens L. from indigenous communities of the Amazon Department (Colombia) were studied to assess the genetic diversity of the collections, and delineate species gene groups. T...


Toquica, SPRodríguez, F.Martínez, E.Duque E., Myriam CristinaTohme, Joseph M.[Molecular characterization by AFLPs of Capsicum germplasm from the Amazon department in Colombia, characterization by AFLPs of Capsicum]Molecular characterization by AFLPs of Capsicum germplasm from the Amazon department in Colombia, characterization by AFLPs of Capsicum

This study evaluated P. edulis f. edulis intraspecific variation at DNA level using AFLPs and SSRs. About 60 specimens were collected from Colombian commercial plantations in the departments of Antioquia, Boyacá, Cundinamarca, Huila, Quindío and Tolima. Ten specimens were also included from Corpo...


Ortiz, Diana CarolinaBohórquez Cháux, AdrianaDuque E., Myriam CristinaTohme, Joseph M.Cuéllar, DavidMosquera Vasquez, Teresa[Evaluating purple passion fruit (Passifloraedulis Sims f. edulis) genetic variability in individuals from commercial plantations in Colombia]Evaluating purple passion fruit (Passifloraedulis Sims f. edulis) genetic variability in individuals from commercial plantations in Colombia

Wild species in varietal improvement of rice In 1994 the CIAT Rice Project launched a pre-breeding program that crossed the species Oryza rufipogon, O. barthii, and O. glaberrima (O. latifolia was recently introduced) with commercial irrigated and upland rice varieties, using a backcrossing schem...

En 1994, el Proyecto de Arroz del CIAT inició un programa de pre-mejoramiento en el que se tomaron las especies Oryza rufipogon, O. barthii y O. glaberrima (recientemente se introdujo O. latifolia) para cruzarlas con variedades comerciales de arroz de riego y de secano, mediante un esquema de ret...


Martínez Racines, César PompilioBorrero Correa, Jaime C.Carabalí, Silvio J.Correa Victoria, Fernando J.Duque E., Myriam Cristina[Las especies silvestres en el mejoramiento varietal del arroz]Las especies silvestres en el mejoramiento varietal del arroz

More than 60% of common bean production worldwide is derived from cultivars of Middle American origin. Understanding the diversity of these will facilitate their use in genetic improvement. The objective of this study was to analyze a collection of 269 landraces of common bean (Phaseolus vulgaris...


Beebe, Stephen E.Skroch, PWTohme, Joseph M.Duque E., Myriam CristinaPedraza García, FabioNienhuis, James[Structure of genetic diversity among common bean landraces of middle American origin based on correspondence analysis of RAPD]Structure of genetic diversity among common bean landraces of middle American origin based on correspondence analysis of RAPD
Blair, Matthew W.Buendía Castellanos, Hector F.Díaz, Lucy MilenaDíaz, J.M.Giraldo Zapata, Martha C.Tovar Luque, EDuque E., Myriam CristinaBeebe, Stephen E.Debouck, Daniel G.[Microsatellite marker diversity in common bean]Microsatellite marker diversity in common bean

A diversity survey was used to estimate allelic diversity and heterozygosity of 129 microsatellite markers in a panel of 44 common bean (Phaseolus vulgaris L.) genotypes that have been used as parents of mapping populations. Two types of microsatellites were evaluated, based respectively on gene ...


Blair, Matthew W.Giraldo Zapata, Martha C.Buendía Castellanos, Hector F.Tovar Luque, EDuque E., Myriam CristinaBeebe, Stephen E.[Microsatellite marker diversity in common bean (Phaseolus vulgaris L.)]Microsatellite marker diversity in common bean (Phaseolus vulgaris L.)

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