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Summary: Xanthomonas axonopodis pv. manihotis (Xam) is the causative agent of cassava bacterial blight (CBB), a worldwide disease that is particularly destructive in South America and Africa. CBB is controlled essentially through the use of resistant varieties. To develop an appropriate disease m...


Restrepo, S.Duque E., Myriam CristinaTohme, Joseph M.Verdier, Valérie[AFLP fingerprinting : An efficient technique for detecting genetic variation of Xanthomonas axonopodis pv. manihotis]AFLP fingerprinting : An efficient technique for detecting genetic variation of Xanthomonas axonopodis pv. manihotis

Despite the worldwide importance of cultivated cassava (M. esculenta Crantz) its origin and taxonomic relationships with other species in the genus have not been clearly established. We evaluated a representative sample of the crop’s diversity and six wild taxa with AFLPs to estimate genetic rela...


Roa, ACMaya, M.M.Duque E., Myriam CristinaTohme, Joseph M.Allem, ACBonierbale, Merideth W.[AFLP analysis of relationships among cassava and other Manihot species]AFLP analysis of relationships among cassava and other Manihot species

Cassava bacterial blight (CBB) is caused by Xanthomonas axonopodis pv. manihotis (Xam). Resistance is found in Manihot esculenta and, in addition, has been introgressed from a wild relative, M. glaziovii. The resistance is thought to be polygenic and additively inherited. Ninety-three varieties o...


Sánchez, G.M.Restrepo, S.Duque E., Myriam CristinaFregene, Martin A.Bonierbale, Merideth W.Verdier, Valérie[AFLP assessment of genetic variability in cassava accessions (Manihot esculenta) resistant and susceptible to the cassava bacterial blight (CBB)]AFLP assessment of genetic variability in cassava accessions (Manihot esculenta) resistant and susceptible to the cassava bacterial blight (CBB)

Amplified fragment length polymophism was assessed in 20 land races and nine elite lines of cassava from Africa, resistant and susceptible to the cassava mosaic disease (CMD). Eleven accessions from a representative core collection from Latin America, previously studied by AFLPs, were included as...


Fregene, Martin A.Bernal, A.Duque E., Myriam CristinaDixon, Alfred G.O.Tohme, Joseph M.[AFLP analysis of African cassava (Manihot esculenta Crantz) germplasm resistant to the cassava mosaic disease (CMD)]AFLP analysis of African cassava (Manihot esculenta Crantz) germplasm resistant to the cassava mosaic disease (CMD)

Microsatellite amplification was performed on cassava (Manihot esculenta) and six other different species (all wild) of the Manihot genus. We used ten pairs of microsatellite primers previously developed from cassava, detecting 124 alleles in a sample of 121 accessions of the seven species. The n...


Roa, ACChavarriaga Aguirre, PaulDuque E., Myriam CristinaMaya, M.M.Bonierbale, Merideth W.Iglesias, C.A.Tohme, Joseph M.[Cross-species amplification of cassava (Manihot esculenta) (Euphorbiaceae) microsatellites: Allelic polymorphism and degree of relationship]Cross-species amplification of cassava (Manihot esculenta) (Euphorbiaceae) microsatellites: Allelic polymorphism and degree of relationship

Cassava bacterial blight, caused by Xanthomonas axonopodis pv. manihotis (Xam) is a destructive disease occurring in most cassava growing-areas. Although Colombian isolates of Xam differ in DNA polymorphism and pathogenicity, no suitable host differentials have been identified to demonstrate phys...


Restrepo, S.Duque E., Myriam CristinaVerdier, Valérie[Characterization of pathotypes among isolates of Xanthomonas axonopodis pv. manihotis in Colombia]Characterization of pathotypes among isolates of Xanthomonas axonopodis pv. manihotis in Colombia

DNA polymorphism and variation in virulence of Xanthomonas axonopodis pv. manihotis (Xam), the causal agent of cassava bacterial blight, were studied within a pathogen population from Venezuela. Collections were made in several fields at different sites within an edaphoclimatic zone where cassava...


Verdier, ValérieRestrepo, S.Mosquera Cifuentes, Gloria MariaDuque E., Myriam CristinaGerstl, ALaberry Saavedra, Rafael Alberto[Genetic and pathogenic variation of Xanthomonas axonopodis pv. manihotis in Venezuela]Genetic and pathogenic variation of Xanthomonas axonopodis pv. manihotis in Venezuela

Cassava (Manihot esculenta Crantz) is a major root crop widely grown in the tropics. Cassava bacterial blight, caused by Xanthomonas axonopodis pv. manihotis (Xam), is an important disease in Latin America and Africa resulting in significant losses. The preferred control method is the use of resi...


López, C.E.Quesada Ocampo, LMBohórquez Cháux, AdrianaDuque E., Myriam CristinaVargas Daza, JaimeTohme, Joseph M.Verdier, Valérie[Mapping EST-derived SSRs and ESTs involved in resistance to bacterial blight in Manihot esculenta]Mapping EST-derived SSRs and ESTs involved in resistance to bacterial blight in Manihot esculenta

We evaluated cassava bacterial blight (CBB) infection in an pair-cross population of 150 individuals derived from an intra-specific cross between two non-inbred cassava (Manihot esculenta Crantz) lines. The replicated trials were carried out in the field under high disease pressure over two conse...


Jorge, VFregene, Martin A.Velez, C.M.Duque E., Myriam CristinaTohme, Joseph M.Verdier, Valérie[QTL analysis of field resistance to Xanthomonas axonopodis pv. manihotis in cassava]QTL analysis of field resistance to Xanthomonas axonopodis pv. manihotis in cassava
Beltrán Giraldo, Jorge AlonsoJaimes, HEcheverry, MLadino Franco, YJLópez, D.Duque E., Myriam CristinaChavarriaga Aguirre, PaulTohme, Joseph M.[Quantitative analysis of transgenes in cassava plants using real-time PCR technology]Quantitative analysis of transgenes in cassava plants using real-time PCR technology

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