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The genetic diversity of 76 accessions of lima bean (Phaseolus lunatus L.), collected mostly from home gardens, was assessed with AFLPs and seed descriptors to evaluate the potential for recovering a lost ex situ collection in Cuba. The sample contained 60 accessions collected from 25 home garden...


Castineiras Alfonso, LGuzmán, F.A.Duque E., Myriam CristinaShagarodsky, TCristobal, RVicente, M.C. de[AFLPs and morphological diversity of Phaseolus lunatus L. in Cuban home gardens : Approaches to recovering the lost ex situ collection]AFLPs and morphological diversity of Phaseolus lunatus L. in Cuban home gardens : Approaches to recovering the lost ex situ collection

Summary: Xanthomonas axonopodis pv. manihotis (Xam) is the causative agent of cassava bacterial blight (CBB), a worldwide disease that is particularly destructive in South America and Africa. CBB is controlled essentially through the use of resistant varieties. To develop an appropriate disease m...


Restrepo, S.Duque E., Myriam CristinaTohme, Joseph M.Verdier, Valérie[AFLP fingerprinting : An efficient technique for detecting genetic variation of Xanthomonas axonopodis pv. manihotis]AFLP fingerprinting : An efficient technique for detecting genetic variation of Xanthomonas axonopodis pv. manihotis

Despite the worldwide importance of cultivated cassava (M. esculenta Crantz) its origin and taxonomic relationships with other species in the genus have not been clearly established. We evaluated a representative sample of the crop’s diversity and six wild taxa with AFLPs to estimate genetic rela...


Roa, ACMaya, M.M.Duque E., Myriam CristinaTohme, Joseph M.Allem, ACBonierbale, Merideth W.[AFLP analysis of relationships among cassava and other Manihot species]AFLP analysis of relationships among cassava and other Manihot species

Cassava bacterial blight (CBB) is caused by Xanthomonas axonopodis pv. manihotis (Xam). Resistance is found in Manihot esculenta and, in addition, has been introgressed from a wild relative, M. glaziovii. The resistance is thought to be polygenic and additively inherited. Ninety-three varieties o...


Sánchez, G.M.Restrepo, S.Duque E., Myriam CristinaFregene, Martin A.Bonierbale, Merideth W.Verdier, Valérie[AFLP assessment of genetic variability in cassava accessions (Manihot esculenta) resistant and susceptible to the cassava bacterial blight (CBB)]AFLP assessment of genetic variability in cassava accessions (Manihot esculenta) resistant and susceptible to the cassava bacterial blight (CBB)

Using AFLPs, 71 peppers (Capsicum) accessions from the species C. chinense Jacq., C. baccatum L., C. annuum L. and C. frutescens L. from indigenous communities of the Amazon Department (Colombia) were studied to assess the genetic diversity of the collections, and delineate species gene groups. T...


Toquica, SPRodríguez, F.Martínez, E.Duque E., Myriam CristinaTohme, Joseph M.[Molecular characterization by AFLPs of Capsicum germplasm from the Amazon department in Colombia, characterization by AFLPs of Capsicum]Molecular characterization by AFLPs of Capsicum germplasm from the Amazon department in Colombia, characterization by AFLPs of Capsicum

More than 60% of common bean production worldwide is derived from cultivars of Middle American origin. Understanding the diversity of these will facilitate their use in genetic improvement. The objective of this study was to analyze a collection of 269 landraces of common bean (Phaseolus vulgaris...


Beebe, Stephen E.Skroch, PWTohme, Joseph M.Duque E., Myriam CristinaPedraza García, FabioNienhuis, James[Structure of genetic diversity among common bean landraces of middle American origin based on correspondence analysis of RAPD]Structure of genetic diversity among common bean landraces of middle American origin based on correspondence analysis of RAPD

A diversity survey was used to estimate allelic diversity and heterozygosity of 129 microsatellite markers in a panel of 44 common bean (Phaseolus vulgaris L.) genotypes that have been used as parents of mapping populations. Two types of microsatellites were evaluated, based respectively on gene ...


Blair, Matthew W.Giraldo Zapata, Martha C.Buendía Castellanos, Hector F.Tovar Luque, EDuque E., Myriam CristinaBeebe, Stephen E.[Microsatellite marker diversity in common bean (Phaseolus vulgaris L.)]Microsatellite marker diversity in common bean (Phaseolus vulgaris L.)

The Andean gene pool of common bean (Phaseolus vulgaris L.) has high levels of morphological diversity in terms of seed color and size, growth habit and agro-ecological adaptation, but previously was characterized by low levels of molecular marker diversity. Three races have been described within...


Blair, Matthew W.Díaz, J.M.Díaz, Lucy MilenaHidalgo, R.Duque E., Myriam Cristina[Microsatellite characterization of Andean races of common bean (Phaseolus vulgaris L.)]Microsatellite characterization of Andean races of common bean (Phaseolus vulgaris L.)

Phaseolus beans are distributed worldwide and are cultivated in the tropics, subtropics, and temperate zones. The common bean is the most important grain legume for direct human consumption in the world. The objectives of this study were to isolate microsatellite repeats and to establish their di...


Gaitán Solís, ElianaDuque E., Myriam CristinaEdwards, K.J.Tohme, Joseph M.[Microsatellite repeats in common bean (Phaseolus vulgaris L.) : Isolation, characterization, and cross-species amplification in Phaseolus ssp]Microsatellite repeats in common bean (Phaseolus vulgaris L.) : Isolation, characterization, and cross-species amplification in Phaseolus ssp

The cassava core collection was selected to represent, with minimum repetitiveness, the potential genetic diversity of the crop. The core (630 accessions) was chosen from the base collection (over 5500 accessions) on the basis of diversity of origin (country and geographic), morphology, isozyme p...


Chavarriaga Aguirre, PaulMaya, M.M.Tohme, Joseph M.Duque E., Myriam CristinaIglesias, C.A.Bonierbale, Merideth W.Kresovich, SKochert, G[Using microsatellites, isozymes and AFLPs to evaluate genetic diversity and redundancy in the cassava core collection and to assess the usefulness of DNA-BASED markers to maintain germplasm collections]Using microsatellites, isozymes and AFLPs to evaluate genetic diversity and redundancy in the cassava core collection and to assess the usefulness of DNA-BASED markers to maintain germplasm collections

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