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The genetic diversity of 76 accessions of lima bean (Phaseolus lunatus L.), collected mostly from home gardens, was assessed with AFLPs and seed descriptors to evaluate the potential for recovering a lost ex situ collection in Cuba. The sample contained 60 accessions collected from 25 home garden...


Castineiras Alfonso, LGuzmán, F.A.Duque E., Myriam CristinaShagarodsky, TCristobal, RVicente, M.C. de[AFLPs and morphological diversity of Phaseolus lunatus L. in Cuban home gardens : Approaches to recovering the lost ex situ collection]AFLPs and morphological diversity of Phaseolus lunatus L. in Cuban home gardens : Approaches to recovering the lost ex situ collection

The taxonomic classification of the wild Lima bean complex needs to be assessed to select species for use in breeding programs and to identify genetic resources for conservation. The objectives of this study were to determine the genetic relationships among, and the phylogeny of, wild Lima bean (...


Caicedo, ALGaitán Solís, ElianaDuque E., Myriam CristinaToro Chica, OrlandoDebouck, Daniel G.Tohme, Joseph M.[AFLP fingerprinting of Phaseolus lunatus L. and related wild species from South America]AFLP fingerprinting of Phaseolus lunatus L. and related wild species from South America

Summary: Xanthomonas axonopodis pv. manihotis (Xam) is the causative agent of cassava bacterial blight (CBB), a worldwide disease that is particularly destructive in South America and Africa. CBB is controlled essentially through the use of resistant varieties. To develop an appropriate disease m...


Restrepo, S.Duque E., Myriam CristinaTohme, Joseph M.Verdier, Valérie[AFLP fingerprinting : An efficient technique for detecting genetic variation of Xanthomonas axonopodis pv. manihotis]AFLP fingerprinting : An efficient technique for detecting genetic variation of Xanthomonas axonopodis pv. manihotis

Despite the worldwide importance of cultivated cassava (M. esculenta Crantz) its origin and taxonomic relationships with other species in the genus have not been clearly established. We evaluated a representative sample of the crop’s diversity and six wild taxa with AFLPs to estimate genetic rela...


Roa, ACMaya, M.M.Duque E., Myriam CristinaTohme, Joseph M.Allem, ACBonierbale, Merideth W.[AFLP analysis of relationships among cassava and other Manihot species]AFLP analysis of relationships among cassava and other Manihot species

Cassava bacterial blight (CBB) is caused by Xanthomonas axonopodis pv. manihotis (Xam). Resistance is found in Manihot esculenta and, in addition, has been introgressed from a wild relative, M. glaziovii. The resistance is thought to be polygenic and additively inherited. Ninety-three varieties o...


Sánchez, G.M.Restrepo, S.Duque E., Myriam CristinaFregene, Martin A.Bonierbale, Merideth W.Verdier, Valérie[AFLP assessment of genetic variability in cassava accessions (Manihot esculenta) resistant and susceptible to the cassava bacterial blight (CBB)]AFLP assessment of genetic variability in cassava accessions (Manihot esculenta) resistant and susceptible to the cassava bacterial blight (CBB)

Amplified fragment length polymophism was assessed in 20 land races and nine elite lines of cassava from Africa, resistant and susceptible to the cassava mosaic disease (CMD). Eleven accessions from a representative core collection from Latin America, previously studied by AFLPs, were included as...


Fregene, Martin A.Bernal, A.Duque E., Myriam CristinaDixon, Alfred G.O.Tohme, Joseph M.[AFLP analysis of African cassava (Manihot esculenta Crantz) germplasm resistant to the cassava mosaic disease (CMD)]AFLP analysis of African cassava (Manihot esculenta Crantz) germplasm resistant to the cassava mosaic disease (CMD)

Random amplified polymorphic DNA (RAPD) markers were used for assessing genetic diversity and its structure in a collection of the drought-tolerant, tropical multipurpose shrub legume Flemingia macrophylla (Willd.) Merrill. The species is especially suited to low-input smallholder production syst...


Andersson, Meike S.Peters, MichaelSchultze-Kraft, RainerGallego Sánchez, Gerardo J.Duque E., Myriam Cristina[Molecular characterization of a collection of the tropical multipurpose shrub legume Flemingia macrophylla]Molecular characterization of a collection of the tropical multipurpose shrub legume Flemingia macrophylla

Using AFLPs, 71 peppers (Capsicum) accessions from the species C. chinense Jacq., C. baccatum L., C. annuum L. and C. frutescens L. from indigenous communities of the Amazon Department (Colombia) were studied to assess the genetic diversity of the collections, and delineate species gene groups. T...


Toquica, SPRodríguez, F.Martínez, E.Duque E., Myriam CristinaTohme, Joseph M.[Molecular characterization by AFLPs of Capsicum germplasm from the Amazon department in Colombia, characterization by AFLPs of Capsicum]Molecular characterization by AFLPs of Capsicum germplasm from the Amazon department in Colombia, characterization by AFLPs of Capsicum
González Torres, Rosa InésSuárez Baron, HaroldMartínez Garay, CarlosDuque E., Myriam CristinaDebouck, Daniel G.Tohme, Joseph M.[Determination of gene flow events in natural "wild-weedy-cultivated" complexes in genepools of Phaseolus lunatus L]Determination of gene flow events in natural "wild-weedy-cultivated" complexes in genepools of Phaseolus lunatus L

This study evaluated P. edulis f. edulis intraspecific variation at DNA level using AFLPs and SSRs. About 60 specimens were collected from Colombian commercial plantations in the departments of Antioquia, Boyacá, Cundinamarca, Huila, Quindío and Tolima. Ten specimens were also included from Corpo...


Ortiz, Diana CarolinaBohórquez Cháux, AdrianaDuque E., Myriam CristinaTohme, Joseph M.Cuéllar, DavidMosquera Vasquez, Teresa[Evaluating purple passion fruit (Passifloraedulis Sims f. edulis) genetic variability in individuals from commercial plantations in Colombia]Evaluating purple passion fruit (Passifloraedulis Sims f. edulis) genetic variability in individuals from commercial plantations in Colombia

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