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Sensitivity and specificity of the FAO/IAEA kits for diagnosis of bovine trypanosomosis were investigated using sera from experimental cattle infected by tsetse challenge with cloned populations of Trypanosoma congolense (three populations) or T. vivax (one population). The kits are based on mono...


Eisler, M.C.Lessard, P.Masake, R.A.Moloo, S.K.Peregrine, A.S.[Sensitivity and specificity of antigen-capture ELISAs for diagnosis of Trypanosoma congolense and Trypansoma vivax infections in cattle]Sensitivity and specificity of antigen-capture ELISAs for diagnosis of Trypanosoma congolense and Trypansoma vivax infections in cattle

The indirect enzyme-linked immunosorbent assay (ELISA) for the detection of anti-trypanosomal antibodies in bovine serum was adapted for use with dried blood spots on filter paper. Aborbance (450 nm) results for samples were expressed as percent positivity, i.e., percentage of the median absorban...


Hopkins, J.S.Chitambo, H.Machila, N.Luckins, A.G.Rae, P.F.Bossche, P. van denEisler, M.C.[Adaptation and validation of antibody-ELISA using dried blood spots on filter paper for epidemiological surveys of tsetse-transmitted trypanosomosis in cattle]Adaptation and validation of antibody-ELISA using dried blood spots on filter paper for epidemiological surveys of tsetse-transmitted trypanosomosis in cattle

Two groups of five Boran (Bos indicus) cattle were infected with one of two populations of Trypanosoma congolense; one drug-sensitive (IL1180), and one drug-resistant (IL3330). The animals were then treated intramuscularly with homidium bromide at a dose rate of 1.0 mg kg - bodyweight 7 days afte...


Murilla, G.A.Peregrine, A.S.Ndung'u, J.M.Holmes, P.H.Eisler, M.C.[The effects of drug-sensitive and drug-resistant Trypanosoma congolense infections on the pharmacokinetics of homidium in Boran cattle]The effects of drug-sensitive and drug-resistant Trypanosoma congolense infections on the pharmacokinetics of homidium in Boran cattle

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