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Mandal, B.B.Chaudhury, R.Engelmann, F.Mal, B.Tao, K.L.Dhillon, B.S.[Conservation biotechnology of plant germplasm: Proceedings of regional training course on in vitro conservation and cryopreservation of plant germplasm, NBPGR, New Delhi, India, 12-25 October 2000]Conservation biotechnology of plant germplasm: Proceedings of regional training course on in vitro conservation and cryopreservation of plant germplasm, NBPGR, New Delhi, India, 12-25 October 2000

During the early 1980s, while searching for ways to more fully utilize the techniques of plant biotechnology, IPGRI (the International Plant Genetic Resources Institute, then the International Board of Plant Genetic Resources, IBPGR) suggested that germplasm be collected by means of in vitro meth...


Pence, V.C.Sandoval, J.A.Villalobos A., V.M.Engelmann, F.International Plant Genetic Resources Institute[In vitro collecting techniques for germplasm conservation]In vitro collecting techniques for germplasm conservation

Plant species that are vegetatively propagated, that have long life cycles and/or produce nonorthodox seeds are traditionally maintained in field collections. Maintaining plants in the field is costly and carries high risks of loss; therefore, the strategies and procedures employed to establish a...


Reed, B.M.Engelmann, F.Dulloo, M.E.Engels, J.M.M.International Plant Genetic Resources Institute[Technical guidelines for the management of field and in vitro germplasm collections]Technical guidelines for the management of field and in vitro germplasm collections

Shoot-tips and somatic embryos are the explants of choice for the in vitro long-term storage of ex situ plant genetic resources in liquid nitrogen. Cryopreservation of organized structures has significantly progressed, especially for species of tropical origin, with the development of several vit...


González Amao, M.T.Panta, A.Roca, William M.Escobar Pérez, Roosevelt H.Engelmann, F.[Development and large scale application of cryopreservation techniques for shoot and somatic embryo cultures of tropical crops]Development and large scale application of cryopreservation techniques for shoot and somatic embryo cultures of tropical crops
Barraco, G.Sylvestre, I.Engelmann, F.[Comparing encapsulation-dehydration and droplet-vitrification for cryopreservation of sugarcane (Saccharum spp.) shoot tips]Comparing encapsulation-dehydration and droplet-vitrification for cryopreservation of sugarcane (Saccharum spp.) shoot tips
Barraco, G.Sylvestre, I.Iapichino, G.Engelmann, F.[Cryopreservation of Limonium serotinum apical meristems from in vitro plantlets using droplet-vitrification]Cryopreservation of Limonium serotinum apical meristems from in vitro plantlets using droplet-vitrification
González Arnao, M.T.Engelmann, F.Abdelnour, A.Aguilar, M.E.Arahana, V.Aranzales Rondón, EricsonArias, A.Clausen, A.Diamante, A.Digilio, A.Dolce, N.Escobar, R.Flachsland, E.Cadeño, J.F.[Current development and application of plant cryopreservation in Latin America and Caribbean]Current development and application of plant cryopreservation in Latin America and Caribbean
Damiano, C.Caboni, E.Frattarelli, A.Condelo, E.Arias, A.Engelmann, F.[Cryopreservation of fruit tree species through encapsulation-dehydration at the CRA: Fruit Research Centre of Rome]Cryopreservation of fruit tree species through encapsulation-dehydration at the CRA: Fruit Research Centre of Rome
Gamez-Pastrana, R.González Arnao, M.T.Martínez Ocampo, Y.Engelmann, F.[Thermal events in calcium alginate beads during encapsulation-dehydration and encapsulation-vitrification protocols]Thermal events in calcium alginate beads during encapsulation-dehydration and encapsulation-vitrification protocols

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