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Escobar Pérez, Roosevelt H.Muñoz, L.Caicedo, EJaramillo, GustavoZapata, DMina, ATohme, Joseph M.[Use of tissue culture techniques to produce disease-free cassava cultivars for small-scale farmers in the department of Cauca, Colombia]Use of tissue culture techniques to produce disease-free cassava cultivars for small-scale farmers in the department of Cauca, Colombia
Escobar Pérez, Roosevelt H.Muñoz, L.Hernández, C.M.Ospina, GCaicedo, ERestrepo, J.Tohme, Joseph M.[Cassava propagation by small scale farmers using a low cost in vitro system]Cassava propagation by small scale farmers using a low cost in vitro system
Aranzales Rondón, EricsonMafla, G.Escobar Pérez, Roosevelt H.Debouck, Daniel G.[Development of in vitro conservation protocols in palms, using Bactris gasipaes Kunth (chontaduro) as a model]Development of in vitro conservation protocols in palms, using Bactris gasipaes Kunth (chontaduro) as a model
Manrique Carpintero, Norma ConstanzaEscobar Pérez, Roosevelt H.Tohme, Joseph M.[Ajuste de las condiciones de crioconservación con materiales de yuca recalcitrantes a la congelación]Ajuste de las condiciones de crioconservación con materiales de yuca recalcitrantes a la congelación
Escobar Pérez, Roosevelt H.Manrique Carpintero, Norma ConstanzaRíos, AMuñoz Flórez, Ligia CarmenzaMafla, G.Debouck, Daniel G.Tohme, Joseph M.[Implementation of the encapsulation-dehydration cryopreservation method for the cassava core collection]Implementation of the encapsulation-dehydration cryopreservation method for the cassava core collection
Escobar Pérez, Roosevelt H.Muñoz, LilianaRios, ANúñez, ATohme, Joseph M.[Using a droplet-vitrification method to partially overcome the recalcitrance of Cassava to cryostorage]Using a droplet-vitrification method to partially overcome the recalcitrance of Cassava to cryostorage

Shoot tips of in vitro-grown plantlets of cassava (Manihot esculenta Crantz), representing a wide range of germplasm, were cryopreserved as follows: precultured for 3 days, cryoprotected and dehydrated for 1 h, then frozen in liquid nitrogen using a six-step protocol. After 3 h in liquid nitrogen...


Escobar Pérez, Roosevelt H.Mafla, G.Roca, William M.[A methodology for recovering cassava plants from shoot tips maintained in liquid nitrogen]A methodology for recovering cassava plants from shoot tips maintained in liquid nitrogen
Thro, Ann MarieRoca, William M.Restrepo, J.Caballero, H.Poats, Susan V.Escobar Pérez, Roosevelt H.Mafla, G.Hernández, C.[Can in vitro biology have farm-level impact for small-scale cassava farmers in Latin America]Can in vitro biology have farm-level impact for small-scale cassava farmers in Latin America

Escobar Pérez, Roosevelt H.Manrique, NMuñoz, L.Rios, ADebouck, Daniel G.Tohme, Joseph M.[Cassava cryopreservation by rapid freezing methodology]Cassava cryopreservation by rapid freezing methodology

The lack of good quality planting material of farmers' cassava varieties, produced locally and at low cost, is a major constraint limiting the expansion of cassava production in Colombia. This article describes the adaptation of conventional cassava propagation to a low-input scheme for rural tis...


Escobar Pérez, Roosevelt H.Hernández, C.M.Larrahondo, NOspina, GIRestrepo M., José M.Muñoz Flórez, Ligia CarmenzaTohme, Joseph M.Roca, William M.[Tissue culture for farmers : Participatory adaptation of low-input cassava propagation in Colombia]Tissue culture for farmers : Participatory adaptation of low-input cassava propagation in Colombia

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