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Escobar Pérez, Roosevelt H.Muñoz, LilianaRios, ANúñez, ATohme, Joseph M.[Using a droplet-vitrification method to partially overcome the recalcitrance of Cassava to cryostorage]Using a droplet-vitrification method to partially overcome the recalcitrance of Cassava to cryostorage

Shoot tips of in vitro-grown plantlets of cassava (Manihot esculenta Crantz), representing a wide range of germplasm, were cryopreserved as follows: precultured for 3 days, cryoprotected and dehydrated for 1 h, then frozen in liquid nitrogen using a six-step protocol. After 3 h in liquid nitrogen...


Escobar Pérez, Roosevelt H.Mafla, G.Roca, William M.[A methodology for recovering cassava plants from shoot tips maintained in liquid nitrogen]A methodology for recovering cassava plants from shoot tips maintained in liquid nitrogen
Thro, Ann MarieRoca, William M.Restrepo, J.Caballero, H.Poats, Susan V.Escobar Pérez, Roosevelt H.Mafla, G.Hernández, C.[Can in vitro biology have farm-level impact for small-scale cassava farmers in Latin America]Can in vitro biology have farm-level impact for small-scale cassava farmers in Latin America

The lack of good quality planting material of farmers' cassava varieties, produced locally and at low cost, is a major constraint limiting the expansion of cassava production in Colombia. This article describes the adaptation of conventional cassava propagation to a low-input scheme for rural tis...


Escobar Pérez, Roosevelt H.Hernández, C.M.Larrahondo, NOspina, GIRestrepo M., José M.Muñoz Flórez, Ligia CarmenzaTohme, Joseph M.Roca, William M.[Tissue culture for farmers : Participatory adaptation of low-input cassava propagation in Colombia]Tissue culture for farmers : Participatory adaptation of low-input cassava propagation in Colombia
Escobar Pérez, Roosevelt H.Santos Meléndez, Luis GuillermoTohme, Joseph M.Roca, William M.[Cryopreservation of FEC lines as an aid for genetic transformation programs]Cryopreservation of FEC lines as an aid for genetic transformation programs
Montoya Moreno, JEEscobar Pérez, Roosevelt H.Chavarriaga Aguirre, PaulTohme, Joseph M.Roca, William M.[Optimizing plant regeneration from friable embryogenic callus using temporary immersion systems]Optimizing plant regeneration from friable embryogenic callus using temporary immersion systems
Escobar Pérez, Roosevelt H.Manrique Carpintero, Norma ConstanzaDebouck, Daniel G.Tohme, Joseph M.Roca, William M.[Cryopreservation of cassava shoot tips using the encapsulation-dehydration technique]Cryopreservation of cassava shoot tips using the encapsulation-dehydration technique
Escobar Pérez, Roosevelt H.Muñoz Flórez, Ligia CarmenzaTohme, Joseph M.Roca, William M.[Rapid propagation of cassava planting material by temporary immersion bioreactors]Rapid propagation of cassava planting material by temporary immersion bioreactors
Escobar Pérez, Roosevelt H.Manrique Carpintero, Norma ConstanzaChavarriaga Aguirre, PaulMuñoz Aguilera, J.E.Ríos, AGallego Sánchez, Gerardo J.Debouck, Daniel G.Tohme, Joseph M.[Aspectos logísticos de manejo y determinación de la estabilidad genética de materiales crioconservados de yuca (Manihot esculenta Crantz)]Aspectos logísticos de manejo y determinación de la estabilidad genética de materiales crioconservados de yuca (Manihot esculenta Crantz)
Escobar Pérez, Roosevelt H.Muñoz Flórez, Ligia CarmenzaRengifo, MMReyes, J.Narváez, GAnaya, BEspitia, AGómez, R.Laignelet, APerry Rubio, STohme, Joseph M.[Implementación y fortalecimiento empresarial de sistemas rurales de propagación in-vitro de yuca y ñame en los municipios de San Jacinto (Bolívar) y Ovejas (Sucre) en Colombia]Implementación y fortalecimiento empresarial de sistemas rurales de propagación in-vitro de yuca y ñame en los municipios de San Jacinto (Bolívar) y Ovejas (Sucre) en Colombia

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