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Shoot-tips and somatic embryos are the explants of choice for the in vitro long-term storage of ex situ plant genetic resources in liquid nitrogen. Cryopreservation of organized structures has significantly progressed, especially for species of tropical origin, with the development of several vit...


González Amao, M.T.Panta, A.Roca, William M.Escobar Pérez, Roosevelt H.Engelmann, F.[Development and large scale application of cryopreservation techniques for shoot and somatic embryo cultures of tropical crops]Development and large scale application of cryopreservation techniques for shoot and somatic embryo cultures of tropical crops

The importance of cassava as the fourth largest source of calories in the world requires that contributions of biotechnology to improving this crop, advances and current challenges, be periodically reviewed. Plant biotechnology offers a wide range of opportunities that can help cassava become a b...


Chavarriaga Aguirre, PaulBrand, AlejandroMedina, AdrianaPrías, MónicaEscobar Pérez, Roosevelt H.Martínez, JuanDíaz, PaulaLópez Carrascal, Camilo ErnestoRoca, William M.Tohme, Joseph M.[The potential of using biotechnology to improve cassava: a review]The potential of using biotechnology to improve cassava: a review
Escobar Pérez, Roosevelt H.Muñoz, LilianaRios, ANúñez, ATohme, Joseph M.[Using a droplet-vitrification method to partially overcome the recalcitrance of Cassava to cryostorage]Using a droplet-vitrification method to partially overcome the recalcitrance of Cassava to cryostorage

Shoot tips of in vitro-grown plantlets of cassava (Manihot esculenta Crantz), representing a wide range of germplasm, were cryopreserved as follows: precultured for 3 days, cryoprotected and dehydrated for 1 h, then frozen in liquid nitrogen using a six-step protocol. After 3 h in liquid nitrogen...


Escobar Pérez, Roosevelt H.Mafla, G.Roca, William M.[A methodology for recovering cassava plants from shoot tips maintained in liquid nitrogen]A methodology for recovering cassava plants from shoot tips maintained in liquid nitrogen
Thro, Ann MarieRoca, William M.Restrepo, J.Caballero, H.Poats, Susan V.Escobar Pérez, Roosevelt H.Mafla, G.Hernández, C.[Can in vitro biology have farm-level impact for small-scale cassava farmers in Latin America]Can in vitro biology have farm-level impact for small-scale cassava farmers in Latin America

The lack of good quality planting material of farmers' cassava varieties, produced locally and at low cost, is a major constraint limiting the expansion of cassava production in Colombia. This article describes the adaptation of conventional cassava propagation to a low-input scheme for rural tis...


Escobar Pérez, Roosevelt H.Hernández, C.M.Larrahondo, NOspina, GIRestrepo M., José M.Muñoz Flórez, Ligia CarmenzaTohme, Joseph M.Roca, William M.[Tissue culture for farmers : Participatory adaptation of low-input cassava propagation in Colombia]Tissue culture for farmers : Participatory adaptation of low-input cassava propagation in Colombia

En el Centro de Investigación Tibaitatá de Corpoica se llevan a cabo ensayos para determinar los mejores métodos de crioconservación de los materiales que integran la Colección Central Colombiana de Papa; ésta se encuentra constituida por 1.098 accesiones de las cuales 151 corresponden a Solanum ...


Villa, A.L.Sánchez, A.M.Valbuena, R.I.Escobar Pérez, Roosevelt H.[Evaluación preliminar de técnicas de crioconservación en una accesión de Solanum phureja = Preliminary evaluation of cryoconservation techniques in an accession of Solanum phureja]Evaluación preliminar de técnicas de crioconservación en una accesión de Solanum phureja = Preliminary evaluation of cryoconservation techniques in an accession of Solanum phureja

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