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Sperm DNA quality and gene expression are crucial for early embryo development. Abnormal genomic processes can cause irreversible damage to totipotent cells, thereby altering the capacity for cell differentiation. Cryopreservation is a complex procedure that exposes cells to extreme conditions; i...


Figueroa, ElíasLee Estévez, ManuelValdebenito, IvánFarías, Jorge G.Romero, Jaime[Potential biomarkers of DNA quality in cryopreserved fish sperm: impact on gene expression and embryonic development]Potential biomarkers of DNA quality in cryopreserved fish sperm: impact on gene expression and embryonic development

Semen conservation methods have been extensively studied for fish species over the last 60years. Cryopreservation techniques can be divided into two types: one with slow cooling rates like traditional freezing and the other with ultrarapid rates as used in vitrification. These protocols increase ...


Figueroa, ElíasFarías, Jorge G.Lee-Estevez, ManuelCerqueira, ViniciusMagnotti, CaioValdebenito, Iván[Cryopreservation and vitrification of fish semen: a review with special emphasis on marine species]Cryopreservation and vitrification of fish semen: a review with special emphasis on marine species

Hypobaric hypoxia induces DNA damage in rat testicular cells, the production of defective spermatozoids and decreased sperm count, associated with an increase in oxidative stress. 8-Oxoguanine glycosylase (OGG1) enzymes are main members of the base excision repair (BER) system, a DNA repair mecha...


Farías, Jorge G.Zepeda, A.Castillo, R.Figueroa Villalobos, ElíasAdemoyero, O.T.Pulgar, V.M[Chronic hypobaric hypoxia diminishes the expression of base excision repair OGG1 enzymes in spermatozoa]Chronic hypobaric hypoxia diminishes the expression of base excision repair OGG1 enzymes in spermatozoa

The knowledge of sperm quality in the broodstock males of different ages is a prerequisite to identify the reproductive ability of cultivated fish for the hatchery management. Thus, in this work, we analysed sperm function of the semen stored of broodstock males of rainbow trout (Oncorhychus myki...


Risopatrón, J.Merino, O.Cheuqueman, C.Figueroa, E.Sánchez, R.Farías, Jorge G.Valdebenito, I.[Effect of the age of broodstock males on sperm function during cold storage in the trout (Oncorhynchus mykiss)]Effect of the age of broodstock males on sperm function during cold storage in the trout (Oncorhynchus mykiss)

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