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Article

Autor: Figueroa Villalobos, Elías Valdebenito Isler, Iván Merino, O. Ubilla Madrid, Andrea Risopatron, J. Farias, J. G.

The objective of this study was to determine the effect of freezing on the function in Atlantic salmon Salmo salar spermatozoa. The semen was frozen in Cortland's medium+1.3M dimethyl sulphoxide+0.3M glucose+2% bovine serum albumin (final concentration) in a ratio of 1:3 (semen:cryoprotectant) as...

Enlace original: http://repositoriodigital.uct.cl/handle/10925/2909

Repositorio Digital Universidad Católica de Temuco
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Figueroa Villalobos, Elías ,  Valdebenito Isler, Iván ,  Merino, O. ,  Ubilla Madrid, Andrea ,  Risopatron, J. ,  Farias, J. G. ,  [Cryopreservation of Atlantic salmon Salmo salar sperm: effects on sperm physiology] ,  Cryopreservation of Atlantic salmon Salmo salar sperm: effects on sperm physiology
Article

Autor: Risopatron, J. Merino, O. Cheuqueman, C. Figueroa Villalobos, Elías Sanchez, R. Farias, J. G. Valdebenito Isler, Iván

The knowledge of sperm quality in the broodstock males of different ages is a prerequisite to identify the reproductive ability of cultivated fish for the hatchery management. Thus, in this work, we analysed sperm function of the semen stored of broodstock males of rainbow trout (Oncorhychus myki...

Enlace original: http://repositoriodigital.uct.cl/handle/10925/2944

Repositorio Digital Universidad Católica de Temuco
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Risopatron, J. ,  Merino, O. ,  Cheuqueman, C. ,  Figueroa Villalobos, Elías ,  Sanchez, R. ,  Farias, J. G. ,  Valdebenito Isler, Iván ,  [Effect of the age of broodstock males on sperm function during cold storage in the trout (Oncorhynchus mykiss)] ,  Effect of the age of broodstock males on sperm function during cold storage in the trout (Oncorhynchus mykiss)
Article

Autor: Valdebenito Isler, Iván Cosson, J. Contreras, P. Sanchez, J. C. Oliveira, R. P. S. Risopatron, J. Farias, J. G. Figueroa Villalobos, Elías

Spermatological research of the Patagonian blennie was carried, specifically biometric parameters, sperm density, sperm count and motility in different activation mediums (815, 716, 590 and 0mOSmkg(-1)), at different temperatures (5, 10 and 15 degrees C) and pH levels (5, 7 and 9). The results in...

Enlace original: http://repositoriodigital.uct.cl/handle/10925/3935

Repositorio Digital Universidad Católica de Temuco
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Valdebenito Isler, Iván ,  Cosson, J. ,  Contreras, P. ,  Sanchez, J. C. ,  Oliveira, R. P. S. ,  Risopatron, J. ,  Farias, J. G. ,  Figueroa Villalobos, Elías ,  [Spermatological research of experimentally farmed Patagonian blenny (Eleginops maclovinus) (Perciformes: Eleginopsidae) in Chile] ,  Spermatological research of experimentally farmed Patagonian blenny (Eleginops maclovinus) (Perciformes: Eleginopsidae) in Chile
Article

Autor: Magnotti, C. Figueroa Villalobos, Elías Farias, J. G. Merino, O. Valdebenito Isler, Iván Oliveira, R. P. S. Cerqueira, V.

In this article we describe basic aspects of the sperm biology of lebranche mullet (Mugil liza) in the wild and in captivity, in particular assessing the effects of salinity (0, 10, 20, 30, 35, 40, 50 and 60 g L-1) and pH (6, 7, 8, 9 and 10) on sperm motility. Our results indicate that the highes...

Enlace original: http://repositoriodigital.uct.cl/handle/10925/3840

Repositorio Digital Universidad Católica de Temuco
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Magnotti, C. ,  Figueroa Villalobos, Elías ,  Farias, J. G. ,  Merino, O. ,  Valdebenito Isler, Iván ,  Oliveira, R. P. S. ,  Cerqueira, V. ,  [Sperm characteristics of wild and captive lebranche mullet Mugil liza (Valenciennes, 1836), subjected to sperm activation in different pH and salinity conditions] ,  Sperm characteristics of wild and captive lebranche mullet Mugil liza (Valenciennes, 1836), subjected to sperm activation in different pH and salinity conditions
Article

Autor: Figueroa Villalobos, Elías Farias, J. G. Lee Estevez, M. Valdebenito Isler, Iván Risopatron, J. Magnotti, C. Romero, J. Watanabe, I Oliveira, R. P. S.

The use of alpha-tocopherol and ascorbic acid in freezing medium prevents lipoperoxidation and damage by ROS (reactive oxygen species); however, the effects of these antioxidants in salmonid spermatozoa have not been studied. In this work the protective effects of antioxidants on the enzymatic ac...

Enlace original: http://repositoriodigital.uct.cl/handle/10925/3926

Repositorio Digital Universidad Católica de Temuco
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Figueroa Villalobos, Elías ,  Farias, J. G. ,  Lee Estevez, M. ,  Valdebenito Isler, Iván ,  Risopatron, J. ,  Magnotti, C. ,  Romero, J. ,  Watanabe, I ,  Oliveira, R. P. S. ,  [Sperm cryopreservation with supplementation of alpha-tocopherol and ascorbic acid in freezing media increase sperm function and fertility rate in Atlantic salmon (Salmo salar)] ,  Sperm cryopreservation with supplementation of alpha-tocopherol and ascorbic acid in freezing media increase sperm function and fertility rate in Atlantic salmon (Salmo salar)
Article

Autor: Figueroa Villalobos, Elías Merino, O. Risopatron, J. Isachenko, V. Sanchez, R. Effer Roldan, Brian Isachenko, E. Farias, J. G. Valdebenito Isler, Iván

This study was designed to test a vitrification method in Atlantic salmon spermatozoa and determine the capacity of seminal plasma (SP) to protect these cells from cryoinjuries. The vitrification medium consisted of a standard buffer for fish spermatozoa (Cortland medium) + 10% DMSO + 2% BSA + 0....

Enlace original: http://repositoriodigital.uct.cl/handle/10925/3702

Repositorio Digital Universidad Católica de Temuco
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Figueroa Villalobos, Elías ,  Merino, O. ,  Risopatron, J. ,  Isachenko, V. ,  Sanchez, R. ,  Effer Roldan, Brian ,  Isachenko, E. ,  Farias, J. G. ,  Valdebenito Isler, Iván ,  [Effect of seminal plasma on Atlantic salmon (Salmo salar) sperm vitrification] ,  Effect of seminal plasma on Atlantic salmon (Salmo salar) sperm vitrification
Article

Autor: Figueroa Villalobos, Elías Lee Estevez, M. Valdebenito Isler, Iván Watanabe, I Oliveira, R. P. S. Romero, J. Castillo, R. L. Farias, J. G.

The development of cryopreservation techniques has led to important changes in animal reproductive biotechnology. However, these techniques are associated with cellular and molecular damage, affecting the mitochondrial function and quality of spermatozoa; moreover studies in fish are limited. In ...

Enlace original: http://repositoriodigital.uct.cl/handle/10925/3826

Repositorio Digital Universidad Católica de Temuco
Enlace permanente
Figueroa Villalobos, Elías ,  Lee Estevez, M. ,  Valdebenito Isler, Iván ,  Watanabe, I ,  Oliveira, R. P. S. ,  Romero, J. ,  Castillo, R. L. ,  Farias, J. G. ,  [Effects of cryopreservation on mitochondrial function and sperm quality in fish] ,  Effects of cryopreservation on mitochondrial function and sperm quality in fish
Article

Autor: Farias, J. G. Zepeda, A. Castillo, R. Figueroa Villalobos, Elías Ademoyero, O. T. Pulgar, V. M.

Hypobaric hypoxia induces DNA damage in rat testicular cells, the production of defective spermatozoids and decreased sperm count, associated with an increase in oxidative stress. 8-Oxoguanine glycosylase (OGG1) enzymes are main members of the base excision repair (BER) system, a DNA repair mecha...

Enlace original: http://repositoriodigital.uct.cl/handle/10925/3953

Repositorio Digital Universidad Católica de Temuco
Enlace permanente
Farias, J. G. ,  Zepeda, A. ,  Castillo, R. ,  Figueroa Villalobos, Elías ,  Ademoyero, O. T. ,  Pulgar, V. M. ,  [Chronic hypobaric hypoxia diminishes the expression of base excision repair OGG1 enzymes in spermatozoa] ,  Chronic hypobaric hypoxia diminishes the expression of base excision repair OGG1 enzymes in spermatozoa

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