In this article we describe basic aspects of the sperm biology of lebranche mullet (Mugil liza) in the wild and in captivity, in particular assessing the effects of salinity (0, 10, 20, 30, 35, 40, 50 and 60 g L−1) and pH (6, 7, 8, 9 and 10) on sperm motility. Our results indicate that the highes...
Magnotti, C.
,
Figueroa Villalobos, Elías
,
Farias, J.G.
,
Merino, O.
,
Valdebenito Isler, Iván
,
Oliveira, R.P.S.
,
Cerqueira, V.
,
[Sperm characteristics of wild and captive lebranche mullet Mugil liza (Valenciennes, 1836), subjected to sperm activation in different pH and salinity conditions]
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Sperm characteristics of wild and captive lebranche mullet Mugil liza (Valenciennes, 1836), subjected to sperm activation in different pH and salinity conditions
Sperm motility in fish with external fertilization is critical for reproductive efficiency in aquaculture, especially in salmonids. Gamete preservation techniques, such as cryopreservation, however, reduce sperm motility and fertilizing capacity. Very few studies have addressed cryodamage from en...
Lee Estévez, M.
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Herrera, L.
,
Díaz, R.
,
Beltrán, J.
,
Figueroa Villalobos, Elías
,
Dumorné, K.
,
Ulloa Rodríguez, P.
,
Short, S.
,
Risopatrón, J.
,
Valdebenito Isler, Iván
,
Farías, J.
,
[Effects of cryopreservation on cAMP-dependent protein kinase and AMP-activated protein kinase in Atlantic salmon (Salmo salar) spermatozoa: Relation with post-thaw motility]
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Effects of cryopreservation on cAMP-dependent protein kinase and AMP-activated protein kinase in Atlantic salmon (Salmo salar) spermatozoa: Relation with post-thaw motility
This study was designed to test a vitrification method on spermatozoa of sex-reversed rainbow trout and determine the ability of sucrose, dimethyl sulfoxide (DMSO), bovine serum albumin (BSA) and seminal plasma (SP) to protect these cells from cryoinjuries. The vitrification medium comprised a st...
Figueroa Villalobos, Elías
,
Risopatron, J.
,
Sanchez, R.
,
Isachenko, E.
,
Merino, O.
,
Isachenko, V.
,
Valdebenito Isler, Iván
,
[Spermatozoa vitrification of sex-reversed rainbow trout (Oncorhynchus mykiss): Effect of seminal plasma on physiological parameters]
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Spermatozoa vitrification of sex-reversed rainbow trout (Oncorhynchus mykiss): Effect of seminal plasma on physiological parameters
Storage of refrigerated semen is a simple and inexpensive procedure that can facilitate the management and reproduction programmes in aquaculture and allows to store semen for in vitro reproduction, quality analyses, hatchery production support, selective in vitro breeding, disease diagnosis, tra...
Contreras, Pablo
,
Dumorne, Kelly
,
Ulloa-Rodriguez, Patricio
,
Merino, Osvaldo
,
Figueroa Villalobos, Elías
,
Farias, Jorge G.
,
Valdebenito Isler, Iván
,
Risopatron, Jennie
,
[Effects of short-term storage on sperm function in fish semen: a review]
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Effects of short-term storage on sperm function in fish semen: a review
Patagonian blenny (E. maclovinus) is a marine species recently placed in captivity and which are potentially farmable. Understanding and improving its sperm capacity to withstand short-term storage conditions is a key element of initiating an artificial propagation program for this species. The a...
Ulloa Rodriguez, Patricio
,
Contreras, Pablo
,
Dumorne, Kelly
,
Lee Estevez, Manuel
,
Diaz, Rommy
,
Figueroa Villalobos, Elías
,
Valdebenito Isler, Iván
,
Risopatron, Jennie
,
Farias, Jorge G.
,
[Patagonian blenny (Eleginops maclovinus) spermatozoa quality after storage at 4 degrees C in Cortland medium]
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Patagonian blenny (Eleginops maclovinus) spermatozoa quality after storage at 4 degrees C in Cortland medium
Sperm motility is considered as a key factor allowing determination of semen quality and predicts fertilizing capacity. In many fish species, the spermatozoa are immotile in the testes and seminal plasma, and motility is induced when they are released in the aqueous environment. Initiation and ac...
The aims of this investigation were to test a novel technology comprising cryoprotectant-free vitrification of the spermatozoa of rainbow trout and to study the ability of sucrose and components of seminal plasma to protect these cells from cryo-injuries. Spermatozoa were isolated and vitrified u...
Merino, O.
,
Sanchez, R.
,
Risopatron, J.
,
Isachenko, E.
,
Katkov, I. I.
,
Figueroa Villalobos, Elías
,
Valdebenito Isler, Iván
,
Mallmann, P.
,
Isachenko, V.
,
[Cryoprotectant-free vitrification of fish (Oncorhynchus mykiss) spermatozoa: first report]
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Cryoprotectant-free vitrification of fish (Oncorhynchus mykiss) spermatozoa: first report
To improve bacteriocin-like inhibitory substance (BLIS) production by Pediococcus pentosaceus ATCC 43200, the influence of pH as well as the addition of sugars-either prebiotic (inulin) or not (sucrose)-on its metabolism were investigated. This strain was grown at pH 5.0 or 6.0 either in glucose-...
de Souza de Azevedo, Pamela Oliveira
,
de Azevedo, Hernando Fernandes
,
Figueroa Villalobos, Elías
,
Converti, Attilio
,
Manuel Dominguez, Jose
,
de Souza Oliveira, Ricardo Pinheiro
,
[Effects of pH and sugar supplements on bacteriocin-like inhibitory substance production by Pediococcus pentosaceus]
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Effects of pH and sugar supplements on bacteriocin-like inhibitory substance production by Pediococcus pentosaceus
Spermatological research of the Patagonian blennie was carried, specifically biometric parameters, sperm density, sperm count and motility in different activation mediums (815, 716, 590 and 0mOSmkg(-1)), at different temperatures (5, 10 and 15 degrees C) and pH levels (5, 7 and 9). The results in...
Valdebenito Isler, Iván
,
Cosson, J.
,
Contreras, P.
,
Sanchez, J. C.
,
Oliveira, R. P. S.
,
Risopatron, J.
,
Farias, J. G.
,
Figueroa Villalobos, Elías
,
[Spermatological research of experimentally farmed Patagonian blenny (Eleginops maclovinus) (Perciformes: Eleginopsidae) in Chile]
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Spermatological research of experimentally farmed Patagonian blenny (Eleginops maclovinus) (Perciformes: Eleginopsidae) in Chile
The aim of the present investigations was to test a novel technology comprising cryoprotectant-free vitrification of the spermatozoa of rainbow trout and to study the ability of sucrose and components of seminal plasma to protect these cells from cryoinjuries. Spermatozoa were isolated and vitrif...
Merino, O.
,
Risopatron, J.
,
Sanchez, R.
,
Isachenko, E.
,
Figueroa Villalobos, Elías
,
Valdebenito Isler, Iván
,
Isachenko, V.
,
[Fish (Oncorhynchus mykiss) spermatozoa cryoprotectant-free vitrification: Stability of mitochondrion as criterion of effectiveness]
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Fish (Oncorhynchus mykiss) spermatozoa cryoprotectant-free vitrification: Stability of mitochondrion as criterion of effectiveness