There is no information about the characteristics of early cleavage in the Patagonian blennie (Eleginops maclovinus), which can be used as a diagnostic tool for embryo quality. The purpose of this investigation, therefore, was to characterize the first blastomeres of E. maclovinus morphologically...
Hypobaric hypoxia induces DNA damage in rat testicular cells, the production of defective spermatozoids and decreased sperm count, associated with an increase in oxidative stress. 8-Oxoguanine glycosylase (OGG1) enzymes are main members of the base excision repair (BER) system, a DNA repair mecha...
Farías, Jorge G.
,
Zepeda, A.
,
Castillo, R.
,
Figueroa Villalobos, Elías
,
Ademoyero, O.T.
,
Pulgar, V.M
,
[Chronic hypobaric hypoxia diminishes the expression of base excision repair OGG1 enzymes in spermatozoa]
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Chronic hypobaric hypoxia diminishes the expression of base excision repair OGG1 enzymes in spermatozoa
The aim of the present investigations was to test a novel technology comprising cryoprotectant-free vitrification of the spermatozoa of rainbow trout and to study the ability of sucrose and components of seminal plasma to protect these cells from cryoinjuries. Spermatozoa were isolated and vitrif...
Merino, O.
,
Risopatrón, J.
,
Sánchez, R.
,
Isachenko, E.
,
Figueroa Villalobos, Elías
,
Valdebenito Isler, Iván
,
Isachenko, V.
,
[Fish (Oncorhynchus mykiss) spermatozoa cryoprotectant-free vitrification: Stability of mitochondrion as criterion of effectiveness]
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Fish (Oncorhynchus mykiss) spermatozoa cryoprotectant-free vitrification: Stability of mitochondrion as criterion of effectiveness
Sperm motility is considered as a key factor allowing determination of semen quality and predicts fertilizing capacity. In many fish species, the spermatozoa are immotile in the testes and seminal plasma, and motility is induced when they are released in the aqueous environment. Initiation and ac...
The use of α-tocopherol and ascorbic acid in freezing medium prevents lipoperoxidation and damage by ROS (reactive oxygen species); however, the effects of these antioxidants in salmonid spermatozoa have not been studied. In this work the protective effects of antioxidants on the enzymatic activi...
Figueroa Villalobos, Elías
,
Farias, J.G.
,
Lee Estevez, M.
,
Valdebenito Isler, Iván
,
Risopatron, J.
,
Magnotti, C.
,
Romero, J.
,
Watanabe, I.
,
Oliveira, R.P.S.
,
[Sperm cryopreservation with supplementation of a-tocopherol and ascorbic acid in freezing media increase sperm function and fertility rate in Atlantic salmon (Salmo salar)]
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Sperm cryopreservation with supplementation of a-tocopherol and ascorbic acid in freezing media increase sperm function and fertility rate in Atlantic salmon (Salmo salar)
In this article we describe basic aspects of the sperm biology of lebranche mullet (Mugil liza) in the wild and in captivity, in particular assessing the effects of salinity (0, 10, 20, 30, 35, 40, 50 and 60 g L−1) and pH (6, 7, 8, 9 and 10) on sperm motility. Our results indicate that the highes...
Magnotti, C.
,
Figueroa Villalobos, Elías
,
Farias, J.G.
,
Merino, O.
,
Valdebenito Isler, Iván
,
Oliveira, R.P.S.
,
Cerqueira, V.
,
[Sperm characteristics of wild and captive lebranche mullet Mugil liza (Valenciennes, 1836), subjected to sperm activation in different pH and salinity conditions]
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Sperm characteristics of wild and captive lebranche mullet Mugil liza (Valenciennes, 1836), subjected to sperm activation in different pH and salinity conditions
Sperm motility in fish with external fertilization is critical for reproductive efficiency in aquaculture, especially in salmonids. Gamete preservation techniques, such as cryopreservation, however, reduce sperm motility and fertilizing capacity. Very few studies have addressed cryodamage from en...
Lee Estévez, M.
,
Herrera, L.
,
Díaz, R.
,
Beltrán, J.
,
Figueroa Villalobos, Elías
,
Dumorné, K.
,
Ulloa Rodríguez, P.
,
Short, S.
,
Risopatrón, J.
,
Valdebenito Isler, Iván
,
Farías, J.
,
[Effects of cryopreservation on cAMP-dependent protein kinase and AMP-activated protein kinase in Atlantic salmon (Salmo salar) spermatozoa: Relation with post-thaw motility]
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Effects of cryopreservation on cAMP-dependent protein kinase and AMP-activated protein kinase in Atlantic salmon (Salmo salar) spermatozoa: Relation with post-thaw motility