Storage of refrigerated semen is a simple and inexpensive procedure that can facilitate the management and reproduction programmes in aquaculture and allows to store semen for in vitro reproduction, quality analyses, hatchery production support, selective in vitro breeding, disease diagnosis, tra...
Contreras, Pablo
,
Dumorne, Kelly
,
Ulloa-Rodriguez, Patricio
,
Merino, Osvaldo
,
Figueroa Villalobos, Elías
,
Farias, Jorge G.
,
Valdebenito Isler, Iván
,
Risopatron, Jennie
,
[Effects of short-term storage on sperm function in fish semen: a review]
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Effects of short-term storage on sperm function in fish semen: a review
Sperm motility is considered as a key factor allowing determination of semen quality and predicts fertilizing capacity. In many fish species, the spermatozoa are immotile in the testes and seminal plasma, and motility is induced when they are released in the aqueous environment. Initiation and ac...
Semen conservation methods have been extensively studied for fish species over the last 60years. Cryopreservation techniques can be divided into two types: one with slow cooling rates like traditional freezing and the other with ultrarapid rates as used in vitrification. These protocols increase ...
Magnotti, Caio
,
Cerqueira, Vinicius
,
Lee Estevez, Manuel
,
Farias, Jorge G.
,
Valdebenito Isler, Iván
,
Figueroa Villalobos, Elías
,
[Cryopreservation and vitrification of fish semen: a review with special emphasis on marine species]
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Cryopreservation and vitrification of fish semen: a review with special emphasis on marine species
The development of sperm cryopreservation has enabled transcendental changes to occur in the reproductive biotechnology of both mammals and fish; it has become a basic tool for animal improvement. Nevertheless, these protocols cause damage to cell structure and physiology, altering sperm function...
Figueroa Villalobos, Elías
,
Valdebenito Isler, Iván
,
Zepeda, Andrea B.
,
Figueroa, Carolina A.
,
Dumorne, Kelly
,
Castillo, Rodrigo L.
,
Farias, Jorge G.
,
[Effects of cryopreservation on mitochondria of fish spermatozoa]
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Effects of cryopreservation on mitochondria of fish spermatozoa
Sperm cryopreservation has led to transcendental changes in the reproductive biotechnology of both mammals and fish, and is a basic tool for animal improvement. However, these protocols generate damage to cell structure and physiology, altering sperm function as a result of cryoinjuries during fr...
Figueroa Villalobos, Elías
,
Valdebenito Isler, Iván
,
Farias, Jorge G.
,
[Technologies used in the study of sperm function in cryopreserved fish spermatozoa]
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Technologies used in the study of sperm function in cryopreserved fish spermatozoa
Sperm DNA quality and gene expression are crucial for early embryo development. Abnormal genomic processes can cause irreversible damage to totipotent cells, thereby altering the capacity for cell differentiation. Cryopreservation is a complex procedure that exposes cells to extreme conditions; i...
Figueroa Villalobos, Elías
,
Lee Estevez, Manuel
,
Valdebenito Isler, Iván
,
Farias, Jorge G.
,
Romero, Jaime
,
[Potential biomarkers of DNA quality in cryopreserved fish sperm: impact on gene expression and embryonic development]
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Potential biomarkers of DNA quality in cryopreserved fish sperm: impact on gene expression and embryonic development
The Zebrafish (Danio Rerio) is a Teleost fish, which offers several practical advantages concerning manipulation, maintenance and versatility as well as short life cycle, low cost and physiological similarity with most farmed species. Zebrafish have been used as model for several aquaculture-rele...
Lee Estevez, Manuel
,
Figueroa Villalobos, Elías
,
Cosson, Jacky
,
Short, Stefania E.
,
Valdebenito Isler, Iván
,
Ulloa Rodriguez, Patricio
,
Farias, Jorge G.
,
[Zebrafish as a useful model for immunological research with potential applications in aquaculture]
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Zebrafish as a useful model for immunological research with potential applications in aquaculture
Oxidative stress (OS) refers to the imbalance between the generation of reactive oxygen species (ROS) and the ability to scavenge these ROS by endogenous antioxidant systems, where ROS overwhelms the antioxidant capacity. Excessive presence of ROS results in irreversible damage to cell membranes,...
Farias, Jorge G.
,
Molina, Victor M.
,
Carrasco, Rodrigo A.
,
Zepeda, Andrea B.
,
Figueroa Villalobos, Elías
,
Letelier, Pablo
,
Castillo, Rodrigo L.
,
[Antioxidant Therapeutic Strategies for Cardiovascular Conditions Associated with Oxidative Stress]
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Antioxidant Therapeutic Strategies for Cardiovascular Conditions Associated with Oxidative Stress