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There is no information about the characteristics of early cleavage in the Patagonian blennie (Eleginops maclovinus), which can be used as a diagnostic tool for embryo quality. The purpose of this investigation, therefore, was to characterize the first blastomeres of E. maclovinus morphologically...


Valdebenito Isler, IvánFigueroa Villalobos, ElíasSandoval Vargas, LeydyContreras Mellado, PabloSánchez Caamaño, Juan CarlosFarías Avendaño, JorgeRisopatrón, Jennie[Characterization of first blastomeres in Patagonian blenny (Eleginops maclovinus) (Perciformes: Eleginopidae)]Characterization of first blastomeres in Patagonian blenny (Eleginops maclovinus) (Perciformes: Eleginopidae)

Hypobaric hypoxia induces DNA damage in rat testicular cells, the production of defective spermatozoids and decreased sperm count, associated with an increase in oxidative stress. 8-Oxoguanine glycosylase (OGG1) enzymes are main members of the base excision repair (BER) system, a DNA repair mecha...


Farías, Jorge G.Zepeda, A.Castillo, R.Figueroa Villalobos, ElíasAdemoyero, O.T.Pulgar, V.M[Chronic hypobaric hypoxia diminishes the expression of base excision repair OGG1 enzymes in spermatozoa]Chronic hypobaric hypoxia diminishes the expression of base excision repair OGG1 enzymes in spermatozoa

The aim of the present investigations was to test a novel technology comprising cryoprotectant-free vitrification of the spermatozoa of rainbow trout and to study the ability of sucrose and components of seminal plasma to protect these cells from cryoinjuries. Spermatozoa were isolated and vitrif...


Merino, O.Risopatrón, J.Sánchez, R.Isachenko, E.Figueroa Villalobos, ElíasValdebenito Isler, IvánIsachenko, V.[Fish (Oncorhynchus mykiss) spermatozoa cryoprotectant-free vitrification: Stability of mitochondrion as criterion of effectiveness]Fish (Oncorhynchus mykiss) spermatozoa cryoprotectant-free vitrification: Stability of mitochondrion as criterion of effectiveness

In this article we describe basic aspects of the sperm biology of Merluccius australis, in particular assessing the effects of pH (6, 8 and 10), temperature (4, 10 and 15 degrees C) and osmolality (922 mOsm/kg, 822 mOsm/kg, 681 mOsm/kg and 3 mOsm/kg) on sperm motility. Our results indicate the pr...


Effer Roldan, BrianFigueroa Villalobos, ElíasAugsburger, A.Valdebenito Isler, Iván[Sperm biology of Merluccius australis: Sperm structure, semen characteristics and effects of pH, temperature and osmolality on sperm motility]Sperm biology of Merluccius australis: Sperm structure, semen characteristics and effects of pH, temperature and osmolality on sperm motility

Sperm cryopreservation is an important tool for reproductive management; however, it has not been possible to perform this procedure for coho salmon. This study was designed to assess the effect of cryopreservation on sperm functional parameters, oxidative stress markers and fertilizing capacity ...


Sandoval Vargas, LeydyDumorne, KellyContreras, PabloFarias, Jorge G.Figueroa Villalobos, ElíasRisopatron, JennieValdebenito Isler, Iván[Cryopreservation of coho salmon sperm (Oncorhynchus kisutch): Effect on sperm function, oxidative stress and fertilizing capacity]Cryopreservation of coho salmon sperm (Oncorhynchus kisutch): Effect on sperm function, oxidative stress and fertilizing capacity

The objective of this study was to determine the effect of freezing on the function in Atlantic salmon Salmo salar spermatozoa. The semen was frozen in Cortland's medium+1.3M dimethyl sulphoxide+0.3M glucose+2% bovine serum albumin (final concentration) in a ratio of 1:3 (semen:cryoprotectant) as...


Figueroa Villalobos, ElíasValdebenito Isler, IvánMerino, O.Ubilla Madrid, AndreaRisopatron, J.Farias, J. G.[Cryopreservation of Atlantic salmon Salmo salar sperm: effects on sperm physiology]Cryopreservation of Atlantic salmon Salmo salar sperm: effects on sperm physiology

The knowledge of sperm quality in the broodstock males of different ages is a prerequisite to identify the reproductive ability of cultivated fish for the hatchery management. Thus, in this work, we analysed sperm function of the semen stored of broodstock males of rainbow trout (Oncorhychus myki...


Risopatron, J.Merino, O.Cheuqueman, C.Figueroa Villalobos, ElíasSanchez, R.Farias, J. G.Valdebenito Isler, Iván[Effect of the age of broodstock males on sperm function during cold storage in the trout (Oncorhynchus mykiss)]Effect of the age of broodstock males on sperm function during cold storage in the trout (Oncorhynchus mykiss)
Diaz, RommyLee-Estevez, ManuelFigueroa Villalobos, ElíasUlloa-Rodriguez, PatricioSepulveda, NestorFarias, Jorge G.[Study of the membrane lipid composition of Atlantic salmon (Salmo salar) spermatozoa and its relation with semen quality]Study of the membrane lipid composition of Atlantic salmon (Salmo salar) spermatozoa and its relation with semen quality

Sperm motility is considered as a key factor allowing determination of semen quality and predicts fertilizing capacity. In many fish species, the spermatozoa are immotile in the testes and seminal plasma, and motility is induced when they are released in the aqueous environment. Initiation and ac...


Durmone, KellyFigueroa Villalobos, ElíasCosson, JackyLee Estevez, ManuelUlloa Rodríguez, PatricioValdebenito, IvánFarias, Jorge G.[Protein phosphorylation and ions effects on salmonid sperm motility activation]Protein phosphorylation and ions effects on salmonid sperm motility activation

The use of α-tocopherol and ascorbic acid in freezing medium prevents lipoperoxidation and damage by ROS (reactive oxygen species); however, the effects of these antioxidants in salmonid spermatozoa have not been studied. In this work the protective effects of antioxidants on the enzymatic activi...


Figueroa Villalobos, ElíasFarias, J.G.Lee Estevez, M.Valdebenito Isler, IvánRisopatron, J.Magnotti, C.Romero, J.Watanabe, I.Oliveira, R.P.S.[Sperm cryopreservation with supplementation of a-tocopherol and ascorbic acid in freezing media increase sperm function and fertility rate in Atlantic salmon (Salmo salar)]Sperm cryopreservation with supplementation of a-tocopherol and ascorbic acid in freezing media increase sperm function and fertility rate in Atlantic salmon (Salmo salar)

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