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Article

Autor: Contreras, P. Ulloa, P. Merino, O. Valdebenito Isler, Iván Figueroa Villalobos, Elías Farias, J. Risopatron, J.

Short-term storage of semen is a useful strategy for preservation of fish spermatozoa in reproductive management, however, no information is available on Patagonian blenny (Eleginops maclovinus) semen. This study was designed to evaluate the effects of in vitro storage on sperm function parameter...

Enlace original: http://repositoriodigital.uct.cl/handle/10925/3211

Repositorio Digital Universidad Católica de Temuco
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Contreras, P. ,  Ulloa, P. ,  Merino, O. ,  Valdebenito Isler, Iván ,  Figueroa Villalobos, Elías ,  Farias, J. ,  Risopatron, J. ,  [Effect of short-term storage on sperm function in Patagonian blenny (Eleginops maclovinus) sperm] ,  Effect of short-term storage on sperm function in Patagonian blenny (Eleginops maclovinus) sperm
Article

Autor: Trigo, P. Merino, O. Figueroa Villalobos, Elías Valdebenito Isler, Iván Sanchez, R. Risopatron, J.

The short-term storage of salmonid semen is a viable method for in vitro fertilisation. Previous studies have found that short-term storage affects sperm motility, compromising quality and fertilising capacity. However, the functional characteristics of the spermatozoa of O.mykiss during storage ...

Enlace original: http://repositoriodigital.uct.cl/handle/10925/3048

Repositorio Digital Universidad Católica de Temuco
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Trigo, P. ,  Merino, O. ,  Figueroa Villalobos, Elías ,  Valdebenito Isler, Iván ,  Sanchez, R. ,  Risopatron, J. ,  [Effect of short-term semen storage in salmon (Oncorhynchus mykiss) on sperm functional parameters evaluated by flow cytometry] ,  Effect of short-term semen storage in salmon (Oncorhynchus mykiss) on sperm functional parameters evaluated by flow cytometry
Article

Autor: Merino, O. Figueroa Villalobos, Elías Cheuqueman, C. Valdebenito Isler, Iván Isachenko, V. Isachenko, E. Sanchez, R. Farias, J. Risopatron, J.

Short-term storage of semen is a useful strategy for preservation of fish spermatozoa. However, there is a significantly decrease on sperm function mainly due to oxidative stress. In this way, sodium alginate plays an important role as free radical scavenger compound. Accordingly, the aim of our ...

Enlace original: http://repositoriodigital.uct.cl/handle/10925/3357

Repositorio Digital Universidad Católica de Temuco
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Merino, O. ,  Figueroa Villalobos, Elías ,  Cheuqueman, C. ,  Valdebenito Isler, Iván ,  Isachenko, V. ,  Isachenko, E. ,  Sanchez, R. ,  Farias, J. ,  Risopatron, J. ,  [Short-term storage of salmonids semen in a sodium alginate-based extender] ,  Short-term storage of salmonids semen in a sodium alginate-based extender
Article

Autor: Beltran, Jorge F. Herrera Belen, Lisandra Lee Estevez, Manuel Figueroa Villalobos, Elías Dumorne, Kelly Farias, Jorge G.

Ca2+ is a key element in the sperm activation process of Salmo salar. However, the molecular mechanisms by which this ion enters the sperm cell have been poorly studied. In this study, we examined, for the first time, the role of the voltage-gated T-type Ca2+ channel in the activation of sperm mo...

Enlace original: http://repositoriodigital.uct.cl/handle/10925/3424

Repositorio Digital Universidad Católica de Temuco
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Beltran, Jorge F. ,  Herrera Belen, Lisandra ,  Lee Estevez, Manuel ,  Figueroa Villalobos, Elías ,  Dumorne, Kelly ,  Farias, Jorge G. ,  [The voltage-gated T-type Ca2+ channel is key to the sperm motility of Atlantic salmon (Salmo salar)] ,  The voltage-gated T-type Ca2+ channel is key to the sperm motility of Atlantic salmon (Salmo salar)
Article

Autor: Dumorne, Kelly Valdebenito Isler, Iván Risopatron, Jennie Figueroa Villalobos, Elías Diaz, Rommy Farias, Jorge

In this study, the morphology and ultrastructure of Genypterus blacodes spermatozoa were characterized through scanning and transmission electron microscopy. Findings revealed that the G. blacodes spermatozoa can be differentiated into three major parts: a spherical head without an acrosome (typi...

Enlace original: http://repositoriodigital.uct.cl/handle/10925/3549

Repositorio Digital Universidad Católica de Temuco
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Dumorne, Kelly ,  Valdebenito Isler, Iván ,  Risopatron, Jennie ,  Figueroa Villalobos, Elías ,  Diaz, Rommy ,  Farias, Jorge ,  [Morphology and ultrastructure of pink cusk-eel (Genypterus blacodes, Schneider 1801) spermatozoa by scanning and transmission electron microscopy] ,  Morphology and ultrastructure of pink cusk-eel (Genypterus blacodes, Schneider 1801) spermatozoa by scanning and transmission electron microscopy
Article

Autor: Dumorne, Kelly Valdebenito Isler, Iván Contreras, Pablo Ulloa Rodriguez, Patricio Risopatron, Jennie Figueroa Villalobos, Elías Estevez, Manuel Lee Diaz, Rommy Farias, Jorge
Enlace original: http://repositoriodigital.uct.cl/handle/10925/3576

Repositorio Digital Universidad Católica de Temuco
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Dumorne, Kelly ,  Valdebenito Isler, Iván ,  Contreras, Pablo ,  Ulloa Rodriguez, Patricio ,  Risopatron, Jennie ,  Figueroa Villalobos, Elías ,  Estevez, Manuel Lee ,  Diaz, Rommy ,  Farias, Jorge ,  [Effect of pH, osmolality and temperature on sperm motility of pink cusk-eel (Genypterus blacodes, (Forster, 1801))] ,  Effect of pH, osmolality and temperature on sperm motility of pink cusk-eel (Genypterus blacodes, (Forster, 1801))
Article

Autor: Figueroa Villalobos, Elías Merino, O. Risopatron, J. Isachenko, V. Sanchez, R. Effer Roldan, Brian Isachenko, E. Farias, J. G. Valdebenito Isler, Iván

This study was designed to test a vitrification method in Atlantic salmon spermatozoa and determine the capacity of seminal plasma (SP) to protect these cells from cryoinjuries. The vitrification medium consisted of a standard buffer for fish spermatozoa (Cortland medium) + 10% DMSO + 2% BSA + 0....

Enlace original: http://repositoriodigital.uct.cl/handle/10925/3702

Repositorio Digital Universidad Católica de Temuco
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Figueroa Villalobos, Elías ,  Merino, O. ,  Risopatron, J. ,  Isachenko, V. ,  Sanchez, R. ,  Effer Roldan, Brian ,  Isachenko, E. ,  Farias, J. G. ,  Valdebenito Isler, Iván ,  [Effect of seminal plasma on Atlantic salmon (Salmo salar) sperm vitrification] ,  Effect of seminal plasma on Atlantic salmon (Salmo salar) sperm vitrification
Article

Autor: Figueroa Villalobos, Elías Lee Estevez, M. Valdebenito Isler, Iván Watanabe, I Oliveira, R. P. S. Romero, J. Castillo, R. L. Farias, J. G.

The development of cryopreservation techniques has led to important changes in animal reproductive biotechnology. However, these techniques are associated with cellular and molecular damage, affecting the mitochondrial function and quality of spermatozoa; moreover studies in fish are limited. In ...

Enlace original: http://repositoriodigital.uct.cl/handle/10925/3826

Repositorio Digital Universidad Católica de Temuco
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Figueroa Villalobos, Elías ,  Lee Estevez, M. ,  Valdebenito Isler, Iván ,  Watanabe, I ,  Oliveira, R. P. S. ,  Romero, J. ,  Castillo, R. L. ,  Farias, J. G. ,  [Effects of cryopreservation on mitochondrial function and sperm quality in fish] ,  Effects of cryopreservation on mitochondrial function and sperm quality in fish
Article

Autor: Lee-Estevez, Manuel Larama, Giovanni Figueroa Villalobos, Elías Ulloa-Rodriguez, Patricio Diaz, Rommy Valdebenito Isler, Iván Farias, Jorge G.

Patagonian blenny (Eleginops maclovinus) is the only member of Eleginopidae family. It has been an important resource for Chilean artisanal fishermen communities, however, capture volumes have progressively decreased over last decades likely because overfishing, and concerns for conservation of t...

Enlace original: http://repositoriodigital.uct.cl/handle/10925/4168

Repositorio Digital Universidad Católica de Temuco
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Lee-Estevez, Manuel ,  Larama, Giovanni ,  Figueroa Villalobos, Elías ,  Ulloa-Rodriguez, Patricio ,  Diaz, Rommy ,  Valdebenito Isler, Iván ,  Farias, Jorge G. ,  [Complete mitochondrial genome sequence of Patagonian blenny, Eleginops maclovinus (Perciformes: Eleginopidae) with phylogenetic consideration] ,  Complete mitochondrial genome sequence of Patagonian blenny, Eleginops maclovinus (Perciformes: Eleginopidae) with phylogenetic consideration
Article

Autor: Farias, J. G. Zepeda, A. Castillo, R. Figueroa Villalobos, Elías Ademoyero, O. T. Pulgar, V. M.

Hypobaric hypoxia induces DNA damage in rat testicular cells, the production of defective spermatozoids and decreased sperm count, associated with an increase in oxidative stress. 8-Oxoguanine glycosylase (OGG1) enzymes are main members of the base excision repair (BER) system, a DNA repair mecha...

Enlace original: http://repositoriodigital.uct.cl/handle/10925/3953

Repositorio Digital Universidad Católica de Temuco
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Farias, J. G. ,  Zepeda, A. ,  Castillo, R. ,  Figueroa Villalobos, Elías ,  Ademoyero, O. T. ,  Pulgar, V. M. ,  [Chronic hypobaric hypoxia diminishes the expression of base excision repair OGG1 enzymes in spermatozoa] ,  Chronic hypobaric hypoxia diminishes the expression of base excision repair OGG1 enzymes in spermatozoa

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