In this study, the morphology and ultrastructure of Genypterus blacodes spermatozoa were characterized through scanning and transmission electron microscopy. Findings revealed that the G. blacodes spermatozoa can be differentiated into three major parts: a spherical head without an acrosome (typi...
Dumorne, Kelly
,
Valdebenito Isler, Iván
,
Risopatron, Jennie
,
Figueroa Villalobos, Elías
,
Diaz, Rommy
,
Farias, Jorge
,
[Morphology and ultrastructure of pink cusk-eel (Genypterus blacodes, Schneider 1801) spermatozoa by scanning and transmission electron microscopy]
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Morphology and ultrastructure of pink cusk-eel (Genypterus blacodes, Schneider 1801) spermatozoa by scanning and transmission electron microscopy
The Zebrafish (Danio Rerio) is a Teleost fish, which offers several practical advantages concerning manipulation, maintenance and versatility as well as short life cycle, low cost and physiological similarity with most farmed species. Zebrafish have been used as model for several aquaculture-rele...
Lee Estevez, Manuel
,
Figueroa Villalobos, Elías
,
Cosson, Jacky
,
Short, Stefania E.
,
Valdebenito Isler, Iván
,
Ulloa Rodriguez, Patricio
,
Farias, Jorge G.
,
[Zebrafish as a useful model for immunological research with potential applications in aquaculture]
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Zebrafish as a useful model for immunological research with potential applications in aquaculture
Dumorne, Kelly
,
Valdebenito Isler, Iván
,
Contreras, Pablo
,
Ulloa Rodriguez, Patricio
,
Risopatron, Jennie
,
Figueroa Villalobos, Elías
,
Estevez, Manuel Lee
,
Diaz, Rommy
,
Farias, Jorge
,
[Effect of pH, osmolality and temperature on sperm motility of pink cusk-eel (Genypterus blacodes, (Forster, 1801))]
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Effect of pH, osmolality and temperature on sperm motility of pink cusk-eel (Genypterus blacodes, (Forster, 1801))
This study was designed to test a vitrification method in Atlantic salmon spermatozoa and determine the capacity of seminal plasma (SP) to protect these cells from cryoinjuries. The vitrification medium consisted of a standard buffer for fish spermatozoa (Cortland medium) + 10% DMSO + 2% BSA + 0....
Figueroa Villalobos, Elías
,
Merino, O.
,
Risopatron, J.
,
Isachenko, V.
,
Sanchez, R.
,
Effer Roldan, Brian
,
Isachenko, E.
,
Farias, J. G.
,
Valdebenito Isler, Iván
,
[Effect of seminal plasma on Atlantic salmon (Salmo salar) sperm vitrification]
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Effect of seminal plasma on Atlantic salmon (Salmo salar) sperm vitrification
The development of cryopreservation techniques has led to important changes in animal reproductive biotechnology. However, these techniques are associated with cellular and molecular damage, affecting the mitochondrial function and quality of spermatozoa; moreover studies in fish are limited. In ...
Figueroa Villalobos, Elías
,
Lee Estevez, M.
,
Valdebenito Isler, Iván
,
Watanabe, I
,
Oliveira, R. P. S.
,
Romero, J.
,
Castillo, R. L.
,
Farias, J. G.
,
[Effects of cryopreservation on mitochondrial function and sperm quality in fish]
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Effects of cryopreservation on mitochondrial function and sperm quality in fish
Patagonian blenny (Eleginops maclovinus) is the only member of Eleginopidae family. It has been an important resource for Chilean artisanal fishermen communities, however, capture volumes have progressively decreased over last decades likely because overfishing, and concerns for conservation of t...
Hypobaric hypoxia induces DNA damage in rat testicular cells, the production of defective spermatozoids and decreased sperm count, associated with an increase in oxidative stress. 8-Oxoguanine glycosylase (OGG1) enzymes are main members of the base excision repair (BER) system, a DNA repair mecha...
Farias, J. G.
,
Zepeda, A.
,
Castillo, R.
,
Figueroa Villalobos, Elías
,
Ademoyero, O. T.
,
Pulgar, V. M.
,
[Chronic hypobaric hypoxia diminishes the expression of base excision repair OGG1 enzymes in spermatozoa]
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Chronic hypobaric hypoxia diminishes the expression of base excision repair OGG1 enzymes in spermatozoa
Oxidative stress (OS) refers to the imbalance between the generation of reactive oxygen species (ROS) and the ability to scavenge these ROS by endogenous antioxidant systems, where ROS overwhelms the antioxidant capacity. Excessive presence of ROS results in irreversible damage to cell membranes,...
Farias, Jorge G.
,
Molina, Victor M.
,
Carrasco, Rodrigo A.
,
Zepeda, Andrea B.
,
Figueroa Villalobos, Elías
,
Letelier, Pablo
,
Castillo, Rodrigo L.
,
[Antioxidant Therapeutic Strategies for Cardiovascular Conditions Associated with Oxidative Stress]
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Antioxidant Therapeutic Strategies for Cardiovascular Conditions Associated with Oxidative Stress