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A genetic linkage map of cassava has been constructed with 132 RFLPs, 30 RAPDs, 3 microsatellites, and 3 isoenzyme markers segregating from the heterozygous female parent of an intraspecific cross. The F1 cross was made between ‘TMS 30572’ and ‘CM?2177-2’, elite cassava cultivars from Nigeria and...


Fregene, Martin A.Angel, F.Gómez, R.Rodríguez, F.Chavarriaga Aguirre, PaulRoca, William M.Tohme, Joseph M.Bonierbale, Merideth W.[A molecular genetic map of cassava (Manihot esculenta Crantz)]A molecular genetic map of cassava (Manihot esculenta Crantz)

Cassava bacterial blight (CBB) is caused by Xanthomonas axonopodis pv. manihotis (Xam). Resistance is found in Manihot esculenta and, in addition, has been introgressed from a wild relative, M. glaziovii. The resistance is thought to be polygenic and additively inherited. Ninety-three varieties o...


Sánchez, G.M.Restrepo, S.Duque E., Myriam CristinaFregene, Martin A.Bonierbale, Merideth W.Verdier, Valérie[AFLP assessment of genetic variability in cassava accessions (Manihot esculenta) resistant and susceptible to the cassava bacterial blight (CBB)]AFLP assessment of genetic variability in cassava accessions (Manihot esculenta) resistant and susceptible to the cassava bacterial blight (CBB)

Amplified fragment length polymophism was assessed in 20 land races and nine elite lines of cassava from Africa, resistant and susceptible to the cassava mosaic disease (CMD). Eleven accessions from a representative core collection from Latin America, previously studied by AFLPs, were included as...


Fregene, Martin A.Bernal, A.Duque E., Myriam CristinaDixon, Alfred G.O.Tohme, Joseph M.[AFLP analysis of African cassava (Manihot esculenta Crantz) germplasm resistant to the cassava mosaic disease (CMD)]AFLP analysis of African cassava (Manihot esculenta Crantz) germplasm resistant to the cassava mosaic disease (CMD)

Fregene, Martin A.Tohme, Joseph M.Roca, William M.Chavarriaga Aguirre, PaulEscobar Pérez, Roosevelt H.Ceballos, H.[Biotecnología para la yuca]Biotecnología para la yuca

Una de las principales estrategias para el manejo del Añublo Bacterial de la Yuca (CBB), causado por Xanthomonas axonopodis pv. manihotis es el uso de resistencia varietal, que implica desarrollar variedades de yuca con resistencia genética duradera. Para tal fin, es necesario conocer los genes q...


Hurtado Lopez, PXÁlvarez, E.Llano Rodríguez, Germán AlbertoFregene, Martin A.[Detección de marcadores microsatélites asociados con la resistencia al añublo bacterial de la yuca (Manihot esculenta Crantz) en Colombia]Detección de marcadores microsatélites asociados con la resistencia al añublo bacterial de la yuca (Manihot esculenta Crantz) en Colombia

We applied the cDNA-AFLP (amplified fragment length polymorphism) technique to mRNA from the parents of a cassava (Manihot esculenta) genetic mapping population, and obtained more than 500 transcript-derived fragments (TDFs) that were unique in either parent. A subset of 50 TDFs were cloned and s...


Suarez, MCBernal, A.Gutiérrez, J.Tohme, Joseph M.Fregene, Martin A.[Developing expressed sequence tags (ESTs) from polymorphic transcript-derived fragments (TDFs) in cassava (Manihot esculenta Crantz)]Developing expressed sequence tags (ESTs) from polymorphic transcript-derived fragments (TDFs) in cassava (Manihot esculenta Crantz)

Cassava mosaic disease (CMD) is the most-important disease of cassava (Manihot esculenta) in Africa, and is a potential threat to Latin American (LA) cassava production. Although this viral disease is still unknown in LA, its vector – the whitefly – has recently been found. The disease is best co...


Akano, A.O.Dixon, Alfred G.O.Mba, CBarrera Sabogal, EFregene, Martin A.[Genetic mapping of a dominant gene conferring resistance to cassava mosaic disease]Genetic mapping of a dominant gene conferring resistance to cassava mosaic disease

Microsatellites or simple sequence repeats (SSR) are the markers of choice for molecular genetic mapping and marker-assisted selection in many crop species. A microsatellite-based linkage map of cassava was drawn using SSR markers and a F2 population consisting of 268 individuals. The F2 populati...


Okogbenin, EmmanuelMarin, JAFregene, Martin A.[An SSR-based molecular genetic map of cassava]An SSR-based molecular genetic map of cassava

The gene pools for breeding cassava (Manihot esculenta Crantz) in Africa currently contain only a fraction of the existing genetic variation found in Latin America where the crop originates. Our research aimed to broaden the genetic base in Africa by introducing Latin American (LA) germplasm. The...


Okogbenin, EmmanuelPorto, M.C.M.Egesi, Chiedozie N.Mba, CEspinosa, E.Santos Melendez, LGOspina, C.Marin, JABarrera Sabogal, EGutiérrez A., Janeth PatriciaEkanayake, I.J.Iglesias, C.A.Fregene, Martin A.[Marker-assisted introgression of resistance to cassava mosaic disease into Latin American germplasm for the genetic improvement of cassava in Africa]Marker-assisted introgression of resistance to cassava mosaic disease into Latin American germplasm for the genetic improvement of cassava in Africa
Álvarez, E.Hurtado López, PXFregene, Martin A.Llano Rodríguez, Germán Alberto[Detection of SRR markers associated with resistance to cassava (Manihot esculenta Crantz) Bacterial Blight (CBB) in Colombia]Detection of SRR markers associated with resistance to cassava (Manihot esculenta Crantz) Bacterial Blight (CBB) in Colombia

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