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A genetic linkage map of cassava has been constructed with 132 RFLPs, 30 RAPDs, 3 microsatellites, and 3 isoenzyme markers segregating from the heterozygous female parent of an intraspecific cross. The F1 cross was made between ‘TMS 30572’ and ‘CM?2177-2’, elite cassava cultivars from Nigeria and...


Fregene, Martin A.Angel, F.Gómez, R.Rodríguez, F.Chavarriaga Aguirre, PaulRoca, William M.Tohme, Joseph M.Bonierbale, Merideth W.[A molecular genetic map of cassava (Manihot esculenta Crantz)]A molecular genetic map of cassava (Manihot esculenta Crantz)

Cassava bacterial blight (CBB) is caused by Xanthomonas axonopodis pv. manihotis (Xam). Resistance is found in Manihot esculenta and, in addition, has been introgressed from a wild relative, M. glaziovii. The resistance is thought to be polygenic and additively inherited. Ninety-three varieties o...


Sánchez, G.M.Restrepo, S.Duque E., Myriam CristinaFregene, Martin A.Bonierbale, Merideth W.Verdier, Valérie[AFLP assessment of genetic variability in cassava accessions (Manihot esculenta) resistant and susceptible to the cassava bacterial blight (CBB)]AFLP assessment of genetic variability in cassava accessions (Manihot esculenta) resistant and susceptible to the cassava bacterial blight (CBB)

Amplified fragment length polymophism was assessed in 20 land races and nine elite lines of cassava from Africa, resistant and susceptible to the cassava mosaic disease (CMD). Eleven accessions from a representative core collection from Latin America, previously studied by AFLPs, were included as...


Fregene, Martin A.Bernal, A.Duque E., Myriam CristinaDixon, Alfred G.O.Tohme, Joseph M.[AFLP analysis of African cassava (Manihot esculenta Crantz) germplasm resistant to the cassava mosaic disease (CMD)]AFLP analysis of African cassava (Manihot esculenta Crantz) germplasm resistant to the cassava mosaic disease (CMD)
Akinbo, OlalekanLabuschagne, M.T.Fregene, Martin A.[Increased storage protein from interspecific F1 hybrids between cassava (Manihot esculenta Crantz) and its wild progenitor (M. esculenta ssp. flabellifolia)]Increased storage protein from interspecific F1 hybrids between cassava (Manihot esculenta Crantz) and its wild progenitor (M. esculenta ssp. flabellifolia)

Chloroplast DNA (cp) and nuclear ribosomal DNA (rDNA) variation was investigated in 45 accessions of cultivated and wild Manihot species. Ten independent mutations, 8 point mutations and 2 length mutations were identified, using eight restriction enzymes and 12 heterologous cpDNA probes from mung...


Fregene, Martin A.Vargas, J.Ikea, J.Angel, F.Thome, J.Asiedu, RobertAkoroda, M.O.Roca, W.[Variability of chloroplast DNA and nuclear ribosomal DNA in cassava (Manihot esculenta Crantz) and its wild relatives]Variability of chloroplast DNA and nuclear ribosomal DNA in cassava (Manihot esculenta Crantz) and its wild relatives

The most widespread disease of economic importance of cassava is caused by whitefly vector, both as a single strain or combination of strains. A B1P2 family was generated from the crosses of an inter-specific F1 hybrid (CW 198-11) as a female parent with a commercial cassava cultivar (MTAI-8) as ...


Akinbo, OlalekanLabuschagne, M.T.Fregene, Martin A.[Introgression of whitefly (Aleurotrachelus socialis) resistance gene from F1 inter specific hybrids into commercial cassava]Introgression of whitefly (Aleurotrachelus socialis) resistance gene from F1 inter specific hybrids into commercial cassava

Una de las principales estrategias para el manejo del Añublo Bacterial de la Yuca (CBB), causado por Xanthomonas axonopodis pv. manihotis es el uso de resistencia varietal, que implica desarrollar variedades de yuca con resistencia genética duradera. Para tal fin, es necesario conocer los genes q...


Hurtado Lopez, PXÁlvarez, E.Llano Rodríguez, Germán AlbertoFregene, Martin A.[Detección de marcadores microsatélites asociados con la resistencia al añublo bacterial de la yuca (Manihot esculenta Crantz) en Colombia]Detección de marcadores microsatélites asociados con la resistencia al añublo bacterial de la yuca (Manihot esculenta Crantz) en Colombia

A study was conducted to determine the extent of genetic diversity among African cassava (Manihot esculenta Crantz) accessions resistant to the cassava mosaic virus disease (CMD), using simple sequence repeat (SSR) markers. The accessions included a breeding stock (clone 58308), five improved lin...


Lokko, Y.Dixon, A.Offei, S.Danquah, E.Y.Fregene, Martin A.[Assessment of genetic diversity among African cassava Manihot esculenta Grantz accessions resistant to the cassava mosaic virus disease using SSR markers]Assessment of genetic diversity among African cassava Manihot esculenta Grantz accessions resistant to the cassava mosaic virus disease using SSR markers

Cassava (Manihot esculenta) is an allogamous, vegetatively propagated, Neotropical crop that is also widely grown in tropical Africa and Southeast Asia. To elucidate genetic diversity and differentiation in the crop's primary and secondary centers of diversity, and the forces shaping them, SSR ma...


Fregene, Martin A.Suarez, M.Mkumbira, J.Kulembeka, H.P.Ndedya, E.Kulaya, A.Mitchel, S.Gullberg, U.Rosling, H.Dixon, Alfred G.O.Dean, R.[Simple sequence repeat marker diversity in cassava landraces: genetic diversity and differentiation in an asexually propagated crop]Simple sequence repeat marker diversity in cassava landraces: genetic diversity and differentiation in an asexually propagated crop

We applied the cDNA-AFLP (amplified fragment length polymorphism) technique to mRNA from the parents of a cassava (Manihot esculenta) genetic mapping population, and obtained more than 500 transcript-derived fragments (TDFs) that were unique in either parent. A subset of 50 TDFs were cloned and s...


Suarez, MCBernal, A.Gutiérrez, J.Tohme, Joseph M.Fregene, Martin A.[Developing expressed sequence tags (ESTs) from polymorphic transcript-derived fragments (TDFs) in cassava (Manihot esculenta Crantz)]Developing expressed sequence tags (ESTs) from polymorphic transcript-derived fragments (TDFs) in cassava (Manihot esculenta Crantz)

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