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A genetic linkage map of cassava has been constructed with 132 RFLPs, 30 RAPDs, 3 microsatellites, and 3 isoenzyme markers segregating from the heterozygous female parent of an intraspecific cross. The F1 cross was made between ‘TMS 30572’ and ‘CM?2177-2’, elite cassava cultivars from Nigeria and...


Fregene, Martin A.Angel, F.Gómez, R.Rodríguez, F.Chavarriaga Aguirre, PaulRoca, William M.Tohme, Joseph M.Bonierbale, Merideth W.[A molecular genetic map of cassava (Manihot esculenta Crantz)]A molecular genetic map of cassava (Manihot esculenta Crantz)

We applied the cDNA-AFLP (amplified fragment length polymorphism) technique to mRNA from the parents of a cassava (Manihot esculenta) genetic mapping population, and obtained more than 500 transcript-derived fragments (TDFs) that were unique in either parent. A subset of 50 TDFs were cloned and s...


Suarez, MCBernal, A.Gutiérrez, J.Tohme, Joseph M.Fregene, Martin A.[Developing expressed sequence tags (ESTs) from polymorphic transcript-derived fragments (TDFs) in cassava (Manihot esculenta Crantz)]Developing expressed sequence tags (ESTs) from polymorphic transcript-derived fragments (TDFs) in cassava (Manihot esculenta Crantz)

Cassava mosaic disease (CMD) is the most-important disease of cassava (Manihot esculenta) in Africa, and is a potential threat to Latin American (LA) cassava production. Although this viral disease is still unknown in LA, its vector – the whitefly – has recently been found. The disease is best co...


Akano, A.O.Dixon, Alfred G.O.Mba, CBarrera Sabogal, EFregene, Martin A.[Genetic mapping of a dominant gene conferring resistance to cassava mosaic disease]Genetic mapping of a dominant gene conferring resistance to cassava mosaic disease

Microsatellites or simple sequence repeats (SSR) are the markers of choice for molecular genetic mapping and marker-assisted selection in many crop species. A microsatellite-based linkage map of cassava was drawn using SSR markers and a F2 population consisting of 268 individuals. The F2 populati...


Okogbenin, EmmanuelMarin, JAFregene, Martin A.[An SSR-based molecular genetic map of cassava]An SSR-based molecular genetic map of cassava

Genetic mapping of early bulking in a full-sib population of cassava was continued in a selfed family of 268 cassava plants derived from a single progeny of the full-sib population. The pseudo F2 population was analysed with 122 segregating SSR markers. A previously constructed linkage map of cas...


Okogbenin, EmmanuelMarin, JFregene, Martin A.[QTL analysis for early yield in a pseudo F2 population of cassava]QTL analysis for early yield in a pseudo F2 population of cassava

Los genes de resistencia se buscaron mediante dos estrategias. La primera, por medio de hibridización con sondas de maíz y arroz, utilizando RFLP. La segunda consistió en la amplificación de regiones conservadas de ADN, con cebadores degenerados NBS y Pto kinasa, en tres genotipos de yuca resiste...


Llano Rodríguez, Germán AlbertoÁlvarez, E.Muñoz, J.E.Fregene, Martin A.[Identificación de genes análogos de resistencia a enfermedades en yuca, Manihot esculenta Crantz, y su relación con la resistencia a tres especies de Phytophthora]Identificación de genes análogos de resistencia a enfermedades en yuca, Manihot esculenta Crantz, y su relación con la resistencia a tres especies de Phytophthora

Cassava bacterial blight (CBB), caused by Xanthomonas axonopodis pv. manihotis (Xam), is a major disease of cassava (Manihot esculenta Crantz) in Africa and South America. Planting resistant varieties is the preferred method of disease control. Recent genetic mapping of an F1 cross (TMS 30572 × C...


Jorge, VFregene, Martin A.Duque E., Myriam CristinaBonierbale, Merideth W.Tohme, Joseph M.Verdier, Valérie[Genetic mapping of resistance to bacterial blight disease in cassava (Manihot esculenta Crantz)]Genetic mapping of resistance to bacterial blight disease in cassava (Manihot esculenta Crantz)

Breeding goals of yield increases, root quality improvement, and disease resistance in cassava are considerably slowed down by biological characteristics of the crop, which includes a long growth cycle, a heterozygous genetic background and a poor knowledge of the organization of crop diversity. ...


Fregene, Martin A.Okogbenin, EmmanuelMba, CAngel, F.Suarez, MCGutiérrez A., Janeth PatriciaChavarriaga Aguirre, PaulRoca, William M.Bonierbale, Merideth W.Tohme, Joseph M.[Genome mapping in cassava improvement: Challenges, achievements and opportunities]Genome mapping in cassava improvement: Challenges, achievements and opportunities

The genetic basis of early bulking in cassava was studied in a replicated, multi-locational trial using 144 F1 progeny derived from an intra-specific cross between two non-inbred parents. A second, sequential harvest experiment examined the relative importance of eight yield-related traits on ear...


Okogbenin, EmmanuelFregene, Martin A.[Genetic analysis and QTL mapping of early root bulking in an F1 population of non-inbred parents in cassava (Manihot esculenta Crantz)]Genetic analysis and QTL mapping of early root bulking in an F1 population of non-inbred parents in cassava (Manihot esculenta Crantz)

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