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The taxonomic classification of the wild Lima bean complex needs to be assessed to select species for use in breeding programs and to identify genetic resources for conservation. The objectives of this study were to determine the genetic relationships among, and the phylogeny of, wild Lima bean (...


Caicedo, ALGaitán Solís, ElianaDuque E., Myriam CristinaToro Chica, OrlandoDebouck, Daniel G.Tohme, Joseph M.[AFLP fingerprinting of Phaseolus lunatus L. and related wild species from South America]AFLP fingerprinting of Phaseolus lunatus L. and related wild species from South America

The Amazon river dolphin, genus Inia, is endemic to the major river basins of northern South America. No previous studies have focused on the genetic structure of this genus. In this work, 96 DNA samples from specimens of this genus were collected in the Orinoco basin (four rivers), the Putumayo ...


Banguera Hinestroza, ECárdenas, H.Ruiz Garcia, MMarmontel, MGaitán Solís, ElianaVazquez, RGarcía Vallejo, F.[Molecular identification of evolutionary significant units in the Amazon river dolphin Inia sp. (Cetacea: Iniidae)]Molecular identification of evolutionary significant units in the Amazon river dolphin Inia sp. (Cetacea: Iniidae)

Wild common bean in the Central Valley of Costa Rica: ecological distribution and molecular characterization. This work offers an update on the distribution of wild common bean in Costa Rica. its ecology and molecular characterization. To date 22 populations have been discovered in four watershed...


González Torres, Rosa InésAraya Villalobos, RodolfoGaitán Solís, ElianaDebouck, Daniel G.[Wild common bean in the central valley of Costa Rica: Ecological distribution and molecular characterization = Frijol silvestre en el Valle Central de Costa Rica: distribución ecológica y caracterización molecular]Wild common bean in the central valley of Costa Rica: Ecological distribution and molecular characterization = Frijol silvestre en el Valle Central de Costa Rica: distribución ecológica y caracterización molecular
González Torres, Rosa InésGaitán Solís, ElianaDuque E., Myriam CristinaToro Chica, OrlandoDebouck, Daniel G.Tohme, Joseph M.[Estimation of gene flow on Phaseolus vulgaris L. using molecular markers : Microsatellites and polymorphisms at chloroplast DNA]Estimation of gene flow on Phaseolus vulgaris L. using molecular markers : Microsatellites and polymorphisms at chloroplast DNA

A polymerase chain reaction approach using degenerate primers that targeted the conserved domains of cloned plant disease resistance genes (R genes) was used to isolate a set of 15 resistance gene analogs (RGAs) from common bean (Phaseolus vulgaris). Eight different classes of RGAs were obtained ...


López, C.E.Acosta, I.F.Jara, Carlos E.Pedraza García, FabioGaitán Solís, ElianaGallego Sánchez, Gerardo J.Beebe, Stephen E.Tohme, Joseph M.[Identifying resistance gene analogs associated with resistances to different pathogens in common beans]Identifying resistance gene analogs associated with resistances to different pathogens in common beans

A total of 150 microsatellite markers developed for common bean (Phaseolus vulgaris L.) were tested for parental polymorphism and used to determine the positions of 100 genetic loci on an integrated genetic map of the species. The value of these single-copy markers was evident in their ability to...


Blair, Matthew W.Pedraza García, FabioBuendía Castellanos, Hector F.Gaitán Solís, ElianaBeebe, Stephen E.Gepts, Paul L.Tohme, Joseph M.[Development of a genome-wide anchored microsatellite map for common bean (Phaseolus vulgaris L.)]Development of a genome-wide anchored microsatellite map for common bean (Phaseolus vulgaris L.)

Phaseolus beans are distributed worldwide and are cultivated in the tropics, subtropics, and temperate zones. The common bean is the most important grain legume for direct human consumption in the world. The objectives of this study were to isolate microsatellite repeats and to establish their di...


Gaitán Solís, ElianaDuque E., Myriam CristinaEdwards, K.J.Tohme, Joseph M.[Microsatellite repeats in common bean (Phaseolus vulgaris L.) : Isolation, characterization, and cross-species amplification in Phaseolus ssp]Microsatellite repeats in common bean (Phaseolus vulgaris L.) : Isolation, characterization, and cross-species amplification in Phaseolus ssp
González Torres, Rosa InésGaitán Solís, ElianaDuque E., Myriam CristinaToro Chica, OrlandoTohme, Joseph M.Debouck, Daniel G.[Medición del flujo de genes en frijol común mediante maracadores moleculares]Medición del flujo de genes en frijol común mediante maracadores moleculares

Single nucleotide polymorphism (SNP) markers are by far the most common form of DNA polymorphism in a genome. The objectives of this study were to discover SNPs in common bean (Phaseolus vulgaris L.) by comparing sequences from coding and noncoding regions obtained from the GenBank and genomic DN...


Gaitán Solís, ElianaChoi, I.Y.Quigley, CCregan, P.B.Tohme, Joseph M.[Single nucleotide polymorphisms in common bean : Their discovery and genotyping using a multiplex detection system]Single nucleotide polymorphisms in common bean : Their discovery and genotyping using a multiplex detection system

González Torres, Rosa InésGaitán Solís, ElianaDuque E., Myriam CristinaToro Chica, OrlandoDebouck, Daniel G.Tohme, Joseph M.[Estimation of gene flow on Phaseolus vulgaris L. using molecular markers: microsatellites and polymorphisms of chloroplast DNA. V Encuentro Latinoamericano y del Caribe de Biotecnología Agrícola, REDBIO, Dominican Republic, 21-25 June 2004.]Estimation of gene flow on Phaseolus vulgaris L. using molecular markers: microsatellites and polymorphisms of chloroplast DNA. V Encuentro Latinoamericano y del Caribe de Biotecnología Agrícola, REDBIO, Dominican Republic, 21-25 June 2004.

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