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Okoth, S.Gallardo, C.Macharia, J.M.Baboon, K.Lekolol, I.Mijele, D.Bishop, Richard P.[Participatory assessment of risk factors for African swine fever in western Kenya]Participatory assessment of risk factors for African swine fever in western Kenya

The role of the ancestral sylvatic cycle of the African swine fever virus (ASFV) is not well understood in the endemic areas of eastern Africa. We therefore analysed the ASF infection status on samples collected from 51 free-ranging warthogs (Phacocherus africanus) and 1576 Ornithodorus porcinus ...


Gallardo, C.Okoth, S.Pelayo, V.Anchuelo, R.Martin, E.Simon, A.Llorente, A.Nieto, R.Soler Garzón, AlvaroMartin, R.Arias, MarisaBishop, Richard P.[African swine fever viruses with two different genotypes, both of which occur in domestic pigs, are associated with ticks and adult warthogs, respectively, at a single geographical site]African swine fever viruses with two different genotypes, both of which occur in domestic pigs, are associated with ticks and adult warthogs, respectively, at a single geographical site

African swine fever virus p72 genotype IX, associated with outbreaks in eastern Africa, is cocirculating in the Republic of the Congo with West African genotype I. Data suggest that viruses from eastern Africa are moving into western Africa, increasing the threat of outbreaks caused by novel viru...


Gallardo, C.Anchuelo, R.Pelayo, V.Poudevigne, F.León, T.Nzoussi, J.Bishop, Richard P.Pérez, C.Soler Garzón, AlvaroNieto, R.Martín, H.Arias, Marisa[African Swine Fever Virus p72 Genotype IX in Domestic Pigs, Congo, 2009]African Swine Fever Virus p72 Genotype IX in Domestic Pigs, Congo, 2009

We describe a horizontal survey of African swine fever virus (ASFV) prevalence and risk factors associated with virus infection in domestic pigs in two contrasting production systems in Kenya. A free range/tethering, low input production system in Ndhiwa District of South-western Kenya is compare...


Okoth, Edward A.Gallardo, C.Macharia, J.M.Omore, Amos O.Pelayo, V.Bulimo, D.W.Arias, MarisaKitala, P.Baboon, K.Lekolol, I.Mijele, D.Bishop, Richard P.[Comparison of African swine fever virus prevalence and risk in two contrasting pig-farming systems in South-west and Central Kenya]Comparison of African swine fever virus prevalence and risk in two contrasting pig-farming systems in South-west and Central Kenya

A highly sensitive and specific real-time PCR method was developed for the reliable and rapid detection of African swine fever virus (ASFV). The method uses a commercial Universal Probe Library (UPL) probe combined with a specifically designed primer set to amplify an ASFV DNA fragment within the...


Fernández Pinero, J.Gallardo, C.Elizalde, M.Robles, A.Gómez, C.Bishop, Richard P.Heath, L.Couacy-Hyman, E.Fasina, F.O.Pelayo, V.Soler Garzón, AlvaroArias, Marisa[Molecular Diagnosis of African Swine Fever by a New Real-Time PCR Using Universal Probe Library]Molecular Diagnosis of African Swine Fever by a New Real-Time PCR Using Universal Probe Library

The presence of antibodies against African swine fever (ASF), a complex fatal notifiable OIE disease of swine, is always indicative of previous infection, since there is no vaccine that is currently used in the field. The early appearance and subsequent long-term persistence of antibodies combine...


Gallardo, C.Soler Garzón, AlvaroNieto, R.Carrascosa, A.L.Mia, G.M. deBishop, Richard P.Martins, C.Fasina, F.O.Couacy-Hyman, E.Heath, L.Pelayo, V.Martin, E.Simon, A.Martin, R.Okurut, A.R.Lekolol, I.Okoth, Edward A.Arias, Marisa[Comparative evaluation of novel African swine fever virus (ASF) antibody detection techniques derived from specific ASF viral genotypes with the OIE internationally prescribed serological tests]Comparative evaluation of novel African swine fever virus (ASF) antibody detection techniques derived from specific ASF viral genotypes with the OIE internationally prescribed serological tests

Twelve complete African swine fever virus (ASFV) genome sequences are currently publicly available and these include only one sequence from East Africa. We describe genome sequencing and annotation of a recent pig-derived p72 genotype IX, and a tick-derived genotype X isolate from Kenya using the...


Bishop, Richard P.Fleischauer, C.Villiers, Etienne P. deOkoth, Edward A.Arias, MarisaGallardo, C.Upton, C.[Comparative analysis of the complete genome sequences of Kenyan African swine fever virus isolates within p72 genotypes IX and X]Comparative analysis of the complete genome sequences of Kenyan African swine fever virus isolates within p72 genotypes IX and X

Samples from infected domestic pigs associated with an outbreak of African swine fever (ASF) in three districts of central Uganda in 2007 were confirmed as being infected with African swine fever virus (ASFV) using a P72 gene-based polymerase chain reaction amplification (PCR) assay combined with...


Gallardo, C.Ademun, A.R.Nieto, R.Nantima, NoelinaArias, MarisaMartin, E.Pelayo, V.Bishop, Richard P.[Genotyping of African swine fever virus (ASFV) isolates associated with disease outbreaks in Uganda in 2007]Genotyping of African swine fever virus (ASFV) isolates associated with disease outbreaks in Uganda in 2007

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