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The role of the ancestral sylvatic cycle of the African swine fever virus (ASFV) is not well understood in the endemic areas of eastern Africa. We therefore analysed the ASF infection status on samples collected from 51 free-ranging warthogs (Phacocherus africanus) and 1576 Ornithodorus porcinus ...


Gallardo, C.Okoth, S.Pelayo, V.Anchuelo, R.Martin, E.Simon, A.Llorente, A.Nieto, R.Soler Garzón, AlvaroMartin, R.Arias, MarisaBishop, Richard P.[African swine fever viruses with two different genotypes, both of which occur in domestic pigs, are associated with ticks and adult warthogs, respectively, at a single geographical site]African swine fever viruses with two different genotypes, both of which occur in domestic pigs, are associated with ticks and adult warthogs, respectively, at a single geographical site

A highly sensitive and specific real-time PCR method was developed for the reliable and rapid detection of African swine fever virus (ASFV). The method uses a commercial Universal Probe Library (UPL) probe combined with a specifically designed primer set to amplify an ASFV DNA fragment within the...


Fernández Pinero, J.Gallardo, C.Elizalde, M.Robles, A.Gómez, C.Bishop, Richard P.Heath, L.Couacy-Hyman, E.Fasina, F.O.Pelayo, V.Soler Garzón, AlvaroArias, Marisa[Molecular Diagnosis of African Swine Fever by a New Real-Time PCR Using Universal Probe Library]Molecular Diagnosis of African Swine Fever by a New Real-Time PCR Using Universal Probe Library

Samples from infected domestic pigs associated with an outbreak of African swine fever (ASF) in three districts of central Uganda in 2007 were confirmed as being infected with African swine fever virus (ASFV) using a P72 gene-based polymerase chain reaction amplification (PCR) assay combined with...


Gallardo, C.Ademun, A.R.Nieto, R.Nantima, NoelinaArias, MarisaMartin, E.Pelayo, V.Bishop, Richard P.[Genotyping of African swine fever virus (ASFV) isolates associated with disease outbreaks in Uganda in 2007]Genotyping of African swine fever virus (ASFV) isolates associated with disease outbreaks in Uganda in 2007

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