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Journal Article 2009-02-10

Autor: Gallardo, C. Mwaengo, D.M. Macharia, J.M. Arias, Marisa Taracha, E.A. Soler Garzón, Alvaro Okoth, S. Martin, E. Kasiti, J. Bishop, Richard P.

Complete sequencing of p54-gene from 67 European, American, and West and East African Swine Fever virus (ASFV) isolates revealed that West African and European ASFV isolates classified within the predominant Genotype I according to partial sequencing of p72 were discriminated into four major sub-...

Enlace original: https://cgspace.cgiar.org/handle/10568/19

Investigación en Agricultura
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Gallardo, C. ,  Mwaengo, D.M. ,  Macharia, J.M. ,  Arias, Marisa ,  Taracha, E.A. ,  Soler Garzón, Alvaro ,  Okoth, S. ,  Martin, E. ,  Kasiti, J. ,  Bishop, Richard P. ,  [Enhanced discrimination of African swine fever virus isolates through nucleotide sequencing of the p54, p72, and pB602L (CVR) genes] ,  Enhanced discrimination of African swine fever virus isolates through nucleotide sequencing of the p54, p72, and pB602L (CVR) genes
Journal Article 2010-06-18

Autor: Fasina, F.O. Shamkai, D. Makinde, A.A. Lombin, L.H. Lazarus, D.D. Rufai, S.A. Adamu, S.S. Agom, D. Pelayo, V. Soler Garzón, Alvaro Simon, A. Adedeji, A.J. Yakubu, M.B. Mantip, S. Benshak, A.J. Okike, I. Anagor, P. Mandeng, D.C. Akanbi, B.O. Ajibade, A.A. Faramade, I. Kazeem, M.M. Enurah, L.U. Bishop, Richard P. Anchuelo, R. Martin, J.H. Gallardo, C.

African swine fever (ASF) has had significant economic and social impact in Nigeria since 1997. However, there has been no effective national response to bring it under control. In this report, we confirm that ASF is still prevalent and widespread in Nigeria. Results from both serosurveillance an...

Enlace original: https://cgspace.cgiar.org/handle/10568/3420

Investigación en Agricultura
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Fasina, F.O. ,  Shamkai, D. ,  Makinde, A.A. ,  Lombin, L.H. ,  Lazarus, D.D. ,  Rufai, S.A. ,  Adamu, S.S. ,  Agom, D. ,  Pelayo, V. ,  Soler Garzón, Alvaro ,  Simon, A. ,  Adedeji, A.J. ,  Yakubu, M.B. ,  Mantip, S. ,  Benshak, A.J. ,  Okike, I. ,  Anagor, P. ,  Mandeng, D.C. ,  Akanbi, B.O. ,  Ajibade, A.A. ,  Faramade, I. ,  Kazeem, M.M. ,  Enurah, L.U. ,  Bishop, Richard P. ,  Anchuelo, R. ,  Martin, J.H. ,  Gallardo, C. ,  [Surveillance for African Swine fever in Nigeria, 2006-2009] ,  Surveillance for African Swine fever in Nigeria, 2006-2009
Journal Article 2010-04-25

Autor: Villiers, Etienne P. de Gallardo, C. Arias, Marisa Silva, M. da Upton, C. Martin, R. Bishop, Richard P.

Viral molecular epidemiology has traditionally analyzed variation in single genes. Whole genome phylogenetic analysis of 123 concatenated genes from 11 ASFV genomes, including E75, a newly sequenced virulent isolate from Spain, identified two clusters. One contained South African isolates from ti...

Enlace original: https://cgspace.cgiar.org/handle/10568/728

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Villiers, Etienne P. de ,  Gallardo, C. ,  Arias, Marisa ,  Silva, M. da ,  Upton, C. ,  Martin, R. ,  Bishop, Richard P. ,  [Phylogenomic analysis of 11 complete African swine fever virus genome sequences] ,  Phylogenomic analysis of 11 complete African swine fever virus genome sequences
Journal Article 2011-03

Autor: Gallardo, C. Okoth, S. Pelayo, V. Anchuelo, R. Martin, E. Simon, A. Llorente, A. Nieto, R. Soler Garzón, Alvaro Martin, R. Arias, Marisa Bishop, Richard P.

The role of the ancestral sylvatic cycle of the African swine fever virus (ASFV) is not well understood in the endemic areas of eastern Africa. We therefore analysed the ASF infection status on samples collected from 51 free-ranging warthogs (Phacocherus africanus) and 1576 Ornithodorus porcinus ...

Enlace original: https://cgspace.cgiar.org/handle/10568/35350

Investigación en Agricultura
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Gallardo, C. ,  Okoth, S. ,  Pelayo, V. ,  Anchuelo, R. ,  Martin, E. ,  Simon, A. ,  Llorente, A. ,  Nieto, R. ,  Soler Garzón, Alvaro ,  Martin, R. ,  Arias, Marisa ,  Bishop, Richard P. ,  [African swine fever viruses with two different genotypes, both of which occur in domestic pigs, are associated with ticks and adult warthogs, respectively, at a single geographical site] ,  African swine fever viruses with two different genotypes, both of which occur in domestic pigs, are associated with ticks and adult warthogs, respectively, at a single geographical site
Journal Article 2011-08-15

Autor: Gallardo, C. Anchuelo, R. Pelayo, V. Poudevigne, F. León, T. Nzoussi, J. Bishop, Richard P. Pérez, C. Soler Garzón, Alvaro Nieto, R. Martín, H. Arias, Marisa

African swine fever virus p72 genotype IX, associated with outbreaks in eastern Africa, is cocirculating in the Republic of the Congo with West African genotype I. Data suggest that viruses from eastern Africa are moving into western Africa, increasing the threat of outbreaks caused by novel viru...

Enlace original: https://cgspace.cgiar.org/handle/10568/12467

Investigación en Agricultura
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Gallardo, C. ,  Anchuelo, R. ,  Pelayo, V. ,  Poudevigne, F. ,  León, T. ,  Nzoussi, J. ,  Bishop, Richard P. ,  Pérez, C. ,  Soler Garzón, Alvaro ,  Nieto, R. ,  Martín, H. ,  Arias, Marisa ,  [African Swine Fever Virus p72 Genotype IX in Domestic Pigs, Congo, 2009] ,  African Swine Fever Virus p72 Genotype IX in Domestic Pigs, Congo, 2009
Journal Article 2011-12-15

Autor: Tignon, M. Gallardo, C. Iscaro, C. Hutet, E. Stede, Y. van der Kolvasov, D. Mia, G.M. de Potier, M.F. le Bishop, Richard P. Arias, Marisa Koenen, F.

A real-time polymerase chain reaction (PCR) assay for the rapid detection of African swine fever virus (ASFV), multiplexed for simultaneous detection of swine beta-actin as an endogenous control, has been developed and validated by four National Reference Laboratories of the European Union for Af...

Enlace original: https://cgspace.cgiar.org/handle/10568/10305

Investigación en Agricultura
PCR
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Tignon, M. ,  Gallardo, C. ,  Iscaro, C. ,  Hutet, E. ,  Stede, Y. van der ,  Kolvasov, D. ,  Mia, G.M. de ,  Potier, M.F. le ,  Bishop, Richard P. ,  Arias, Marisa ,  Koenen, F. ,  [Development and inter-laboratory validation study of an improved new real-time PCR assay with internal control for detection and laboratory diagnosis of African swine fever virus] ,  Development and inter-laboratory validation study of an improved new real-time PCR assay with internal control for detection and laboratory diagnosis of African swine fever virus
Journal Article 2013-06-01

Autor: Okoth, Edward A. Gallardo, C. Macharia, J.M. Omore, Amos O. Pelayo, V. Bulimo, D.W. Arias, Marisa Kitala, P. Baboon, K. Lekolol, I. Mijele, D. Bishop, Richard P.

We describe a horizontal survey of African swine fever virus (ASFV) prevalence and risk factors associated with virus infection in domestic pigs in two contrasting production systems in Kenya. A free range/tethering, low input production system in Ndhiwa District of South-western Kenya is compare...

Enlace original: https://cgspace.cgiar.org/handle/10568/32847

Investigación en Agricultura
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Okoth, Edward A. ,  Gallardo, C. ,  Macharia, J.M. ,  Omore, Amos O. ,  Pelayo, V. ,  Bulimo, D.W. ,  Arias, Marisa ,  Kitala, P. ,  Baboon, K. ,  Lekolol, I. ,  Mijele, D. ,  Bishop, Richard P. ,  [Comparison of African swine fever virus prevalence and risk in two contrasting pig-farming systems in South-west and Central Kenya] ,  Comparison of African swine fever virus prevalence and risk in two contrasting pig-farming systems in South-west and Central Kenya
Journal Article 2013-02-15

Autor: Fernández Pinero, J. Gallardo, C. Elizalde, M. Robles, A. Gómez, C. Bishop, Richard P. Heath, L. Couacy-Hyman, E. Fasina, F.O. Pelayo, V. Soler Garzón, Alvaro Arias, Marisa

A highly sensitive and specific real-time PCR method was developed for the reliable and rapid detection of African swine fever virus (ASFV). The method uses a commercial Universal Probe Library (UPL) probe combined with a specifically designed primer set to amplify an ASFV DNA fragment within the...

Enlace original: https://cgspace.cgiar.org/handle/10568/35270

Investigación en Agricultura
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Fernández Pinero, J. ,  Gallardo, C. ,  Elizalde, M. ,  Robles, A. ,  Gómez, C. ,  Bishop, Richard P. ,  Heath, L. ,  Couacy-Hyman, E. ,  Fasina, F.O. ,  Pelayo, V. ,  Soler Garzón, Alvaro ,  Arias, Marisa ,  [Molecular Diagnosis of African Swine Fever by a New Real-Time PCR Using Universal Probe Library] ,  Molecular Diagnosis of African Swine Fever by a New Real-Time PCR Using Universal Probe Library
Journal Article 2013-02-22

Autor: Gallardo, C. Soler Garzón, Alvaro Nieto, R. Carrascosa, A.L. Mia, G.M. de Bishop, Richard P. Martins, C. Fasina, F.O. Couacy-Hyman, E. Heath, L. Pelayo, V. Martin, E. Simon, A. Martin, R. Okurut, A.R. Lekolol, I. Okoth, Edward A. Arias, Marisa

The presence of antibodies against African swine fever (ASF), a complex fatal notifiable OIE disease of swine, is always indicative of previous infection, since there is no vaccine that is currently used in the field. The early appearance and subsequent long-term persistence of antibodies combine...

Enlace original: https://cgspace.cgiar.org/handle/10568/21687

Investigación en Agricultura
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Gallardo, C. ,  Soler Garzón, Alvaro ,  Nieto, R. ,  Carrascosa, A.L. ,  Mia, G.M. de ,  Bishop, Richard P. ,  Martins, C. ,  Fasina, F.O. ,  Couacy-Hyman, E. ,  Heath, L. ,  Pelayo, V. ,  Martin, E. ,  Simon, A. ,  Martin, R. ,  Okurut, A.R. ,  Lekolol, I. ,  Okoth, Edward A. ,  Arias, Marisa ,  [Comparative evaluation of novel African swine fever virus (ASF) antibody detection techniques derived from specific ASF viral genotypes with the OIE internationally prescribed serological tests] ,  Comparative evaluation of novel African swine fever virus (ASF) antibody detection techniques derived from specific ASF viral genotypes with the OIE internationally prescribed serological tests
Journal Article 2015-04-01

Autor: Bishop, Richard P. Fleischauer, C. Villiers, Etienne P. de Okoth, Edward A. Arias, Marisa Gallardo, C. Upton, C.

Twelve complete African swine fever virus (ASFV) genome sequences are currently publicly available and these include only one sequence from East Africa. We describe genome sequencing and annotation of a recent pig-derived p72 genotype IX, and a tick-derived genotype X isolate from Kenya using the...

Enlace original: https://cgspace.cgiar.org/handle/10568/66095

Investigación en Agricultura
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Bishop, Richard P. ,  Fleischauer, C. ,  Villiers, Etienne P. de ,  Okoth, Edward A. ,  Arias, Marisa ,  Gallardo, C. ,  Upton, C. ,  [Comparative analysis of the complete genome sequences of Kenyan African swine fever virus isolates within p72 genotypes IX and X] ,  Comparative analysis of the complete genome sequences of Kenyan African swine fever virus isolates within p72 genotypes IX and X

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