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We describe a horizontal survey of African swine fever virus (ASFV) prevalence and risk factors associated with virus infection in domestic pigs in two contrasting production systems in Kenya. A free range/tethering, low input production system in Ndhiwa District of South-western Kenya is compare...


Okoth, Edward A.Gallardo, C.Macharia, J.M.Omore, Amos O.Pelayo, V.Bulimo, D.W.Arias, MarisaKitala, P.Baboon, K.Lekolol, I.Mijele, D.Bishop, Richard P.[Comparison of African swine fever virus prevalence and risk in two contrasting pig-farming systems in South-west and Central Kenya]Comparison of African swine fever virus prevalence and risk in two contrasting pig-farming systems in South-west and Central Kenya

A highly sensitive and specific real-time PCR method was developed for the reliable and rapid detection of African swine fever virus (ASFV). The method uses a commercial Universal Probe Library (UPL) probe combined with a specifically designed primer set to amplify an ASFV DNA fragment within the...


Fernández Pinero, J.Gallardo, C.Elizalde, M.Robles, A.Gómez, C.Bishop, Richard P.Heath, L.Couacy-Hyman, E.Fasina, F.O.Pelayo, V.Soler Garzón, AlvaroArias, Marisa[Molecular Diagnosis of African Swine Fever by a New Real-Time PCR Using Universal Probe Library]Molecular Diagnosis of African Swine Fever by a New Real-Time PCR Using Universal Probe Library

The presence of antibodies against African swine fever (ASF), a complex fatal notifiable OIE disease of swine, is always indicative of previous infection, since there is no vaccine that is currently used in the field. The early appearance and subsequent long-term persistence of antibodies combine...


Gallardo, C.Soler Garzón, AlvaroNieto, R.Carrascosa, A.L.Mia, G.M. deBishop, Richard P.Martins, C.Fasina, F.O.Couacy-Hyman, E.Heath, L.Pelayo, V.Martin, E.Simon, A.Martin, R.Okurut, A.R.Lekolol, I.Okoth, Edward A.Arias, Marisa[Comparative evaluation of novel African swine fever virus (ASF) antibody detection techniques derived from specific ASF viral genotypes with the OIE internationally prescribed serological tests]Comparative evaluation of novel African swine fever virus (ASF) antibody detection techniques derived from specific ASF viral genotypes with the OIE internationally prescribed serological tests

Twelve complete African swine fever virus (ASFV) genome sequences are currently publicly available and these include only one sequence from East Africa. We describe genome sequencing and annotation of a recent pig-derived p72 genotype IX, and a tick-derived genotype X isolate from Kenya using the...


Bishop, Richard P.Fleischauer, C.Villiers, Etienne P. deOkoth, Edward A.Arias, MarisaGallardo, C.Upton, C.[Comparative analysis of the complete genome sequences of Kenyan African swine fever virus isolates within p72 genotypes IX and X]Comparative analysis of the complete genome sequences of Kenyan African swine fever virus isolates within p72 genotypes IX and X

Samples from infected domestic pigs associated with an outbreak of African swine fever (ASF) in three districts of central Uganda in 2007 were confirmed as being infected with African swine fever virus (ASFV) using a P72 gene-based polymerase chain reaction amplification (PCR) assay combined with...


Gallardo, C.Ademun, A.R.Nieto, R.Nantima, NoelinaArias, MarisaMartin, E.Pelayo, V.Bishop, Richard P.[Genotyping of African swine fever virus (ASFV) isolates associated with disease outbreaks in Uganda in 2007]Genotyping of African swine fever virus (ASFV) isolates associated with disease outbreaks in Uganda in 2007

Desde marzo 1991 a marzo 1992 se sembraron cada semana 15 muestras de material fecal fresco de equinos adultos en un potrero, determinando el numero de huevos de estrongilidos por gramo (phg). Despues de 1, 2, 3, 4, 6, 8, 10, 12, 16, 20, 24, 28 y 32 semanas de permanencia en el medio se determino...


Sievers, G.Quintana, I.Anticevic, S.Patino, M.Gallardo, C.[Desarrollo, traslacion y sobrevivencia de larvas de estrongilidos del equino en el ambiente natural en Valdivia, Chile]Desarrollo, traslacion y sobrevivencia de larvas de estrongilidos del equino en el ambiente natural en Valdivia, Chile

Se necropsiaron 31 caninos, sin historia clinica de patologias hepaticas o renales. Previo a la eutanasia, realizada con solucion sobresaturada de tiopental sodico, se obtuvo una muestra de plasma; una vez efectuada la eutanasia, se obtuvo una muestra de humor acuoso desde el ojo derecho y otra 2...


Gallardo, C.Paredes, E.Perez, J.[Estudio histopatologico de higado y riñon de caninos y su relacion con las concentraciones de urea, creatinina, proteinas, enzimas (ALT y SAP) en sangre premortem y en humor acuoso a las 0 y 24 horas postmortem]Estudio histopatologico de higado y riñon de caninos y su relacion con las concentraciones de urea, creatinina, proteinas, enzimas (ALT y SAP) en sangre premortem y en humor acuoso a las 0 y 24 horas postmortem

Gallardo, C.Palma Vásquez, Ramón Eduardo.[Relación entre la estructura genética del sapo andino Rhinella spinulosa (Anura: Bufonidae) y el paisaje del norte de Chile (21°- 24° S)]Relación entre la estructura genética del sapo andino Rhinella spinulosa (Anura: Bufonidae) y el paisaje del norte de Chile (21°- 24° S)

Gallardo, C.Correa Quezada, Claudio Luis.[Validation of a cheap and simple nondestructive method for obtaining AFLPs and DNA sequences (mitochondrial and nuclear) in amphibians]Validation of a cheap and simple nondestructive method for obtaining AFLPs and DNA sequences (mitochondrial and nuclear) in amphibians

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