Cassava is a widely grown staple in Sub-Saharan Africa and consumed as a cheap source of calories, but the crop is deficient in micronutrients including pro-vitamin A carotenoids. This challenge is currently being addressed through biofortification breeding that relies on phenotypic selection. Ge...
Udoh, L.I.
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Gedil, M.
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Parkes, E.Y.
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Kulakow, P.A.
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Adesoye, A.I.
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Nwuba, C.
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Rabbi, Ismail Y.
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[Candidate gene sequencing and validation of SNP markers linked to carotenoid content in cassava (Manihot esculenta Crantz)]
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Candidate gene sequencing and validation of SNP markers linked to carotenoid content in cassava (Manihot esculenta Crantz)
Cross contamination arising from random natural processes and breeding errors is one of the prominent challenges to breeding in many crops including cassava (Manihot esculenta). This study attempts to identify molecular marker tools suitable for verifying the genetic purity of putative progenies ...
Otti, G.
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Fakoya, A.
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Andrew, I.
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Gedil, M.
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[Development of genomic tools for verification of hybrids and selfed progenies in cassava (Manihot esculenta)]
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Development of genomic tools for verification of hybrids and selfed progenies in cassava (Manihot esculenta)
Some genetic studies using molecular methods such as diversity assessment or marker-assisted selection require collection of a large number of samples from fields located in the vicinity or in remote areas, followed by isolation of good quality DNA in a short time span. In the present study, diff...
Bhattacharjee, R.
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Ferguson, M.
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Gedil, M.
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Dumet, D.
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Ingelbrecht, I.L.
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[Field collection,preservation and large scale DNA extraction procedures for cassava (Manihot esculenta Crantz.)]
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Field collection,preservation and large scale DNA extraction procedures for cassava (Manihot esculenta Crantz.)
Molecular-marker-aided evaluation of germplasm plays an important role indefining the genetic diversity of plant genotypes for genetic and populationimprovement studies. A collection of African cassava landraces and elite culti-vars was analysed for genetic diversity using 20 amplified fragment l...
Raji, A.A.J.
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Fawole, I.
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Gedil, M.
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Dixon, Alfred G.O.
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[Genetic differentiation analysis of African cassava (Manihot esculenta) landraces and elite germplasm using amplified fragment length polymorphism and simple sequence repeat markers]
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Genetic differentiation analysis of African cassava (Manihot esculenta) landraces and elite germplasm using amplified fragment length polymorphism and simple sequence repeat markers
Cassava, Manihot esculenta, is one of the major food crops in sub-Saharan Africa (SSA) providing the bulk of dietary calories to hundreds of millions of households. Two viral diseases, namely cassava mosaic disease (CMD) and cassava brown streak disease (CBSD) pose a serious threat to cassava pro...
Gedil, M.
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Kumar, M.
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Igwe, D.
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[Isolation and characterization of resistant gene analogs in cassava, wild Manihot species, and castor bean (Ricinus communis)]
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Isolation and characterization of resistant gene analogs in cassava, wild Manihot species, and castor bean (Ricinus communis)
Cassava (Manihot esculenta L.) is one of the most important food crops in the tropics, but yields are far below their potential. The gene pool of cassava contains natural genetic diversity relevant to many important breeding goals, but breeding progress has been slow, partly because of insuf-fici...
Rabbi, Ismail Y.
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Hamblin, M.
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Gedil, M.
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Kulakow, P.A.
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Ferguson, M.
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Ikpan, A.
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Ly, D.
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Jannink, Jean-Luc
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[Genetic mapping using genotypingbysequencing in the clonally propagated cassava]
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Genetic mapping using genotypingbysequencing in the clonally propagated cassava
In a bid to fast-track the process of selection of cassava (Manihot esculenta Crantz) genotypes resistant to the cassava mosaic disease (CMD), caused by whitefly-transmitted begomoviruses, a number of molecular markers have been developed to map resistance genes. The simple sequence repeat (SSR) ...
Gedil, M.
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Otti, G.
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Enok, L.
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Kumar, L.
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[Development and validation of genomic tools for marker-assisted breeding of cassava mosaic disease resistance in cassava (Manihot esculenta)]
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Development and validation of genomic tools for marker-assisted breeding of cassava mosaic disease resistance in cassava (Manihot esculenta)
Discovery of single nucleotide polymorphisms, (SNPs) as a tool in marker-assisted breeding of cassava, Manihot esculenta was studied. The principal objective of this study is to discover single nucleotide polymorphisms (SNPs) in some panels of elite lines (ELs) of cassava and in some mapping popu...
Gedil, M.
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Igwe, D.
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[Discovery of Single Nucleotide Polymorphisms (SNPs) as a tool for markerassisted breeding of cassava, Manihot esculenta]
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Discovery of Single Nucleotide Polymorphisms (SNPs) as a tool for markerassisted breeding of cassava, Manihot esculenta
Before implementation of genomic selection, evaluation of the potential accuracy of prediction can be obtained by cross-validation. In this procedure, a population with both phenotypes and genotypes is split into training and validation sets. The prediction model is fitted using the training set,...
Ly, D.
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Hamblin, M.
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Rabbi, Ismail Y.
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Gedil, M.
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Bakare, M.A.
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Gauch Jr. H.
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Okechukwu, R.U.
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Dixon, A.
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Kulakow, P.A.
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Jannink, Jean-Luc
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[Relatedness and genotype x environment interaction affect prediction accuracies in genomic selection: a study in cassava]
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Relatedness and genotype x environment interaction affect prediction accuracies in genomic selection: a study in cassava
In the last 45 years, IITA has played a pivotal role in the genetic improvement of cassava for resourcepoor farmers in sub-Saharan Africa (SSA). More than 400 varieties have been developed that are not only
high yielding but also resistant to diseases and pests. Many of these improved varieties h...
Rabbi, Ismail Y.
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Gedil, M.
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Ferguson, M.
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Kulakow, P.A.
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[Cassava improvement in the era of "agrigenomics": the road to nextgeneration breeding.]
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Cassava improvement in the era of "agrigenomics": the road to nextgeneration breeding.