Isometamidium chloride has remained a very important prophylactic and therapeutic drug against trypanosomosis in cattle since its introduction into the market in the 1950s with, unfortunately, a concomitant development of resistance in trypanosomosis endemic areas. Amplified Fragment Length Polym...
Delespaux, V.
,
Geysen, D.
,
Majiwa, P.A.O.
,
Geerts, S.
,
[Identification of a genetic marker for isometamidium chloride resistance in Trypanosoma congolense]
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Identification of a genetic marker for isometamidium chloride resistance in Trypanosoma congolense
A survey to investigate resistance to drugs used in the treatment of bovine trypanosomosis was conducted in the eastern province of Zambia between 1996 and 1998. A cross-sectional study was conducted in three districts (Petauke, Katete, Lundazi) at 34 village sampling sites selected at random fr...
Sinyangwe, L.
,
Delespaux, V.
,
Brandt, J.
,
Geerts, S.
,
Mubanga, J.
,
Machila, N.
,
Holmes, P.H.
,
Eisler, M.C.
,
[Trypanocidal drug resistance in eastern province of Zambia]
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Trypanocidal drug resistance in eastern province of Zambia
Surveys were carried out in the slaughterhouses of Abuko and Banjul and on 16 farms involving 391 cattle in the Central River Division of The Gambia. Using conventional meat inspection, 12 (0.75%) out of 1595 slaughter cattle were found infected with cysticerci of Taenia saginata. On-farm screeni...
Testing of trypanocidal drug sensitivity in stabilates of pathogenic trypanosomes derived from cattle continues to be problematic, in spite of Development of in vitro techniques, since these are generally complicated and costly for the resources available in developing country laboratories. Furth...
Geerts, S.
,
Ndung'u, J.M.
,
Murilla, G.
,
Mbwambo, H.
,
Sinyangwe, L.
,
Machila, N.
,
Delespaux, V.
,
Brandt, J.
,
Peregrine, A.S.
,
McDermott, John J.
,
Holmes, P.H.
,
Eisler, M.C.
,
[A simplified cost-effective method for area-wide testing of trypanocidal drug sensitivity of T. congolense in mice]
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A simplified cost-effective method for area-wide testing of trypanocidal drug sensitivity of T. congolense in mice
Resistance to the drugs used to control African animal trypanosomosis is increasingly recognised as a constraint to livestock production in sub-Saharan Africa. The most commonly used tests for detection of trypanocidal drug resistance are tests using mice or ruminants, but these suffer from lack ...
Eisler, M.C.
,
Brandt, J.
,
Bauer, B.
,
Clausen, P.H.
,
Delespaux, V.
,
Holmes, P.H.
,
Ilemobade, A.
,
Machila, N.
,
Mbwambo, H.
,
McDermott, John J.
,
Mehlitz, D.
,
Murilla, G.
,
Ndung'u, J.M.
,
Peregrine, A.S.
,
Sidibe, I.
,
Sinyangwe, L.
,
Geerts, S.
,
[Standardised tests in mice and cattle for the detection of drug resistance in tsetse-transmitted trypanosomes of African domestic cattle]
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Standardised tests in mice and cattle for the detection of drug resistance in tsetse-transmitted trypanosomes of African domestic cattle
In order to compare the prophylactic effect provided by a poly(D,L-lactide) sustained-release device (SRD) containing isometamidium (ISMM; Trypamidium) with that provided by the classical im injection of the drug, a field trial was carried out at the Madina Diassa Ranch in Mali, beginning in Nove...
Diarra, B.
,
Diall, O.
,
Geerts, S.
,
Kageruka, P.
,
Lemmouchi, Y.
,
Schacht, E.
,
Eisler, M.C.
,
Holmes, P.
,
[Field evaluation of the prophylactic effect of an isometamidium sustained-release device against trypanosomiasis in cattle]
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Field evaluation of the prophylactic effect of an isometamidium sustained-release device against trypanosomiasis in cattle
Trypanocidal drugs are the most widely applied method of trypanosomosis control in Africa and the only control method in Latin America and Asia . Treatment recommendations and strategies for chemotherapeutic control of trypanosomosis depends on information of trypanosomosis risk and the prevalenc...
Eisler, M.C.
,
McDermott, John J.
,
Mdachi, R.
,
Murilla, G.
,
Sinyangwe, L.
,
Mubanga, J.
,
Machila, N.
,
Mbwambo, H.
,
Coleman, P.G.
,
Clausen, P.H.
,
Bauer, B.
,
Sidibe, I.
,
Geerts, S.
,
Holmes, P.H.
,
Peregrine, A.S.
,
[Rapid method for the assessment of trypanocidal drug resistance in the field]
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Rapid method for the assessment of trypanocidal drug resistance in the field
The extent and significance of trypanocidal drug resistance was investigated in priority areas of three East and Southern African Countries under a project. The project was built on the results of two previous projects. Existing methods currently available for the demonstration of drug-resistance...
Eisler, M.C.
,
Ndung'u, J.M.
,
Murilla, G.
,
Mdachi, R.M.
,
Mbwambo, H.
,
Sinyangwe, L.
,
Machila, N.
,
Delespaux, V.
,
Geerts, S.
,
Brandt, J.
,
Peregrine, A.S.
,
McDermott, John J.
,
Holmes, P.H.
,
[Area-wide appraisal of drug resistance in trypanosomes infecting cattle in East and Southern Africa]
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Area-wide appraisal of drug resistance in trypanosomes infecting cattle in East and Southern Africa
Besides the in vitro test, two tests are very commonly used for the detection of trypanocidal drug resistance: the tests in mice and in ruminants. Since it has been shown that several parameters such as the number of trypanosomes inoculated and the time of treatment might influence the outcome of...
Geerts, S.
,
Ndung'u, J.M.
,
Murilla, G.
,
Mbwambo, H.
,
Sirryangwe, L.
,
Machila, N.
,
Delespaux, V.
,
Brandt, J.
,
Peregrine, A.S.
,
McDermott, John J.
,
Holmes, P.H.
,
Eisler, M.C.
,
[In vivo tests for the detection of resistance to trypanocidal drugs: Tests in mice and in ruminants]
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In vivo tests for the detection of resistance to trypanocidal drugs: Tests in mice and in ruminants