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Potato virus Y (PVY) is causing yield and quality losses forcing farmers to renew periodically their seeds from clean stocks. Two loci for extreme resistance to PVY, one on chromosome XI and the other on XII, have been identified and used in breeding. The latter corresponds to a well-known source...


Herrera, M.R.Jara Vidalon, L.Montenegro, J.D.Riccio, C.Guzmán, F.Bartolini, I.Ghislain, M.[Molecular and genetic characterization of the Ry adg locus on chromosome XI from Andigena potatoes conferring extreme resistance to potato virus Y.]Molecular and genetic characterization of the Ry adg locus on chromosome XI from Andigena potatoes conferring extreme resistance to potato virus Y.

Embryo-like structures were induced from ‘whole leaf’ explants of sixteen of the twenty investigated sweet potato cultivars on Murashige and Skoog (1962) medium supplemented with 2,4 Dichlorophenoxyacetic acid (0.2 mg/L) at the Makerere University Agricultural Research Institute Tissue culture la...


Sefasi, A.Ghislain, M.Kiggundu, R.Ssemakula, G.N.Rukarwa, R.Kreuze, J.F.Mukasa, S.Mwanga, R.O.M.[Embryo-like structures and root regeneration induced by 2, 4-dichlorophenoxyacetic acid in twenty African sweet potato cultivars.]Embryo-like structures and root regeneration induced by 2, 4-dichlorophenoxyacetic acid in twenty African sweet potato cultivars.

Resistance to antibiotics mediated by selectable marker genes remains a powerful selection tool for transgenic event production. However, regulatory agencies and consumer concerns favor these to be eliminated from food crops. Several excision systems exist but none have been optimized or shown to...


Cuéllar, Wilmer J.Gaudin, A.Solorzano, D.Casas, A.Nopo, L.Chudalayandi, P.Medrano, G.Kreuze, J.F.Ghislain, M.[Self-excision of the antibiotic resistance gene npII using a heat inducible Cre-loxP system from transgenic potato.]Self-excision of the antibiotic resistance gene npII using a heat inducible Cre-loxP system from transgenic potato.

Wu, S.Lau, K.H.Cao, Q.Hamilton, J.P.Sun, H.Zhou, C.Eserman, L.Gemenet, D.Olukolu, B.A.Wang, H.Crisovan, E.Godden, G.Jiao, C.Wang, X.Kitavi, M.Manrique-Carpintero, N.Vaillancourt, B.Wiegert-Rininger, K.Yang, X.Bao, K.Schaff, J.Kreuze, J.F.Gruneberg, W.J.Khan, A.Ghislain, M.Ma, D.Jiang, J.Mwanga, R.O.M.Leebens-Mack, J.Coin, L.Yencho, G.C.Buell, R.Fei, Z.[Genome sequences of two diploid wild relatives of cultivated sweetpotato reveal targets for genetic improvement.]Genome sequences of two diploid wild relatives of cultivated sweetpotato reveal targets for genetic improvement.
Autor: Ghislain, M.

This flyer summarizes the objectives, achievements and plans of CIP’s work to address the problem of weevils through development of weevil resistant sweetpotato varieties through breeding and biotechnology. It outlines the Bt-RNAi combined gene technology being applied, and the progress made betw...


Ghislain, M.[Weevil resistant sweetpotato through biotechnology.]Weevil resistant sweetpotato through biotechnology.
Spooner, D.M.Jansky, S.Clausen, A.Herrera, M.R.Ghislain, M.[The enigma of Solanum maglia in the origin of the Chilean cultivated potato, Solanum tuberosum Chilotanum Group.]The enigma of Solanum maglia in the origin of the Chilean cultivated potato, Solanum tuberosum Chilotanum Group.

Average potato yields in sub-Saharan Africa are four times lower than those in industrialized nations, mostly due to the effects of diseases, particularly late blight. This research demonstrates that the transfer from wild potato relatives of three resistance-conferring genes into a cultivated po...


Ghislain, M.Barekye, A.[Bioengineered potato: Resistance to late blight disease and higher crop yields. Research Brief 01.]Bioengineered potato: Resistance to late blight disease and higher crop yields. Research Brief 01.

Sweetpotato (Ipomoea batatas) is one of the most important food crops in tropical and subtropical countries. In Sub-Saharan Africa, sweetpotato is mainly produced for consumption and as a source of income by resource-poor farmers. However, their production is limited by severe damage caused by pe...


Manrique Trujillo, S.Wamalwa, N.L.Díaz, D.Reano, R.Tovar, J.C.Prentice, K.Ormachea, M.Kreuze, J.F.Machuka, J.Ghislain, M.[Enhancing resistance against African weevils through development of transgenic sweetpotato cultivars (Ipomoea batatas (L.) Lam.) expressing cry7Aa1, cry3Ca1 and ET33-34 genes.]Enhancing resistance against African weevils through development of transgenic sweetpotato cultivars (Ipomoea batatas (L.) Lam.) expressing cry7Aa1, cry3Ca1 and ET33-34 genes.

The cultivated potato, Solanum tuberosum is affected by a variety of diseases with late blight (LB) caused by Phytophthora infestans being the most severe. The disease is mostly controlled by the application of large quantities of fungicides, which represent a financial burden on farmers indevelo...


Mwathi, M.Roman, M.Orbegozo, J.Rivera, C.Forbes, G.Ghislain, M.[Genetic transformatin of potato with a triple R gene construct to confer resistance to late blight.]Genetic transformatin of potato with a triple R gene construct to confer resistance to late blight.

Sefasi, A.Ghislain, M.Kiggundu, A.Ssemakula, G.N.Rukarwa, R.Mukasa, S.[Thidiazuron improves adventitious bud and shoot regeneration in recalcitrant sweetpotato.]Thidiazuron improves adventitious bud and shoot regeneration in recalcitrant sweetpotato.

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