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Kairo, A.Gobright, E.I.Fairlamb, A.H.Nene, Vishvanath[A 7.1 Kb linear DNA molecule of Theileria parva has scrambled rDNA sequences and open reading frames for mitochondrially encoded proteins]A 7.1 Kb linear DNA molecule of Theileria parva has scrambled rDNA sequences and open reading frames for mitochondrially encoded proteins

Initial comparison of 2 polymorphic, immunodominant molecule (PIM) cDNA sequences from Theileria parva suggested that the PIM genes consist of conserved 5' and 3' termini flanking a central variable region. Further evidence is presented, based on sequence analysis, supporting this general structu...


Toye, Philip G.Gobright, E.I.Nyanjui, J.Nene, VishvanathBishop, Richard P.[Structure and sequence variation of the genes encoding the polymorphic, immunodominant molecule (PlM), a candidate diagnostic antigen of Theileria parva]Structure and sequence variation of the genes encoding the polymorphic, immunodominant molecule (PlM), a candidate diagnostic antigen of Theileria parva

Bacteriophage clones containing Theileria parva genomic DNA derived from two different telomeres were isolated and the nucleotide sequences of the telomeric repeats and adjacent telomere-associated (TAS) DNA were determined. The T. parva telomeric repeat sequences, a tandem array of TTTTAGGG or T...


Sohanpal, B.K.Morzaria, S.P.Gobright, E.I.Bishop, Richard P.[Characterisation of the telomeres at opposite ends of a 3 Mb Theileria parva chromosome]Characterisation of the telomeres at opposite ends of a 3 Mb Theileria parva chromosome

Minisatellite tandem repeat elements are well known components of vertebrate genomes, but have not yet been extensively characterized in lower eukaryotes. We describe two unusual. AT-rich minisatellites of the protozoan parasite Theileria parva whose sequences are unrelated to the G/C-rich `chi m...


Bishop, Richard P.Morzaria, S.P.Gobright, E.I.[Linkage of two distinct AT-rich minisatellites at multiple loci in the genome of Theileria parva]Linkage of two distinct AT-rich minisatellites at multiple loci in the genome of Theileria parva

Polymorphic, multicopy gene families are frequently located in subtelomeric regions of the genomes of parasitic protozoa. Theileria parva telomere-associated (TA) DNA from two chromosomes contained long open reading frames (ORFs) 54% identical at the N-termini whose ends were 2670 and 2680 bp fro...


Bishop, Richard P.Gobright, E.I.Nene, VishvanathMorzaria, S.P.Musoke, A.J.Sohanpal, B.K.[Polymorphic open reading frames encoding secretory proteins are located less than 3 kilobases from Theileria parva telomeres]Polymorphic open reading frames encoding secretory proteins are located less than 3 kilobases from Theileria parva telomeres

Two distinct genes encoding single domain, ATP-binding cassette transport protein homologues of Theileria parva were cloned and sequenced. Neither of the genes is tandemly duplicated. One gene, TpABCI, encodes a predicted protein of 593 amino acids with an N-terminal hydrophobic domain containing...


Kibe, M.K.Macklin, M.D.Gobright, E.I.Bishop, Richard P.Urakawa, T.Ole-MoiYoi, O.K.[Characterisation of single domain ATP-binding cassette protein homologues of Theileria parva]Characterisation of single domain ATP-binding cassette protein homologues of Theileria parva

Concerted evolution of multicopy gene families in vertebrates is recognized as an important force in the generation of biological novelty but has not been documented for the multicopy genes of protozoa. A multicopy locus, Tpr, which consists of tandemly arrayed open reading frames (ORFs) containi...


Bishop, Richard P.Musoke, A.J.Morzaria, S.P.Sohanpal, B.K.Gobright, E.I.[Concerted evolution at a multicopy locus in the protozoan parasite Theileria parva: Extreme divergence of potential protein-coding sequences]Concerted evolution at a multicopy locus in the protozoan parasite Theileria parva: Extreme divergence of potential protein-coding sequences

The nucleotide sequences of the large subunit ribosomal RNA coding genes within the two single copy ribosomal DNA transcription units of a cloned Theileria parva isolate from a buffalo were determined. The two LSU rRNA coding units differed by 11 nucleotide substitutions and two deletions of 1 an...


Bishop, Richard P.Gobright, E.I.Spooner, P.R.Allsopp, B.A.Sohanpal, B.K.Collins, N.[Microsequence heterogeneity and expression of LSU rRNA genes within the two single copy ribosomal transcription units of Theileria parva]Microsequence heterogeneity and expression of LSU rRNA genes within the two single copy ribosomal transcription units of Theileria parva

Theileria parva, a tick-transmitted protozoan parasite related to Plasmodium spp., causes the disease East Coast fever, an acute and usually fatal lymphoproliferative disorder of cattle in Africa. Previous studies using sera from cattle that have survived infection identified a polymorphic immuno...


Toye, Philip G.Metzelaar, M.J.Wijngaard, P.L.J.Nene, VishvanathIams, K.P.Roose, J.Nyanjui, J.K.Gobright, E.I.Musoke, A.J.Clevers, H.C.[Characterization of the gene encoding the polymorphic immunodominant molecule, a neutralizing antigen of Theileria parva]Characterization of the gene encoding the polymorphic immunodominant molecule, a neutralizing antigen of Theileria parva
Nene, VishvanathIams, K.P.Gobright, E.I.Musoke, A.J.[Characterisation of the gene encoding a candidate vaccine antigen of Theileria parva sporozoites]Characterisation of the gene encoding a candidate vaccine antigen of Theileria parva sporozoites

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