Two bovine bacterial artificial chromosome clones were screened by PCR to amplify the tumour necrosis factor alpha (TNFA) gene. DNA extraction and Southern blotting and probing with a (GT)10 oligonucleotide were performed, followed by cloning into the pUC19 vector. A 0.9 kb fragment containing se...
Wambugu, J.
,
Kanguha, E.
,
Hanotte, Olivier H.
,
Davis, S.
,
Taylor, J.
,
Skow, L.
,
Brunner, R.
,
Schwerin, M.
,
Teale, A.J.
,
Iraqi, F.A.
,
[Identification of two polymorphic microsatellite repeats on a bovine TNFA-containing BAC clone]
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Identification of two polymorphic microsatellite repeats on a bovine TNFA-containing BAC clone
In this study we examined the genetic diversity of yak populations in the northernmost part of their current global distribution. Five Mongolian and one Russian yak populations as well as one Chinese yak population from the Qinghai-Tibetan Plateau, the putative centre of yak domestication, were a...
Xuebin, Q.
,
Han Jianlin
,
Lkhagva, B.
,
Chekarova, I.
,
Badamdorj, D.
,
Rege, J.E.O.
,
Hanotte, Olivier H.
,
[Genetic diversity and differentiation of Mongolian and Russian yak populations]
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Genetic diversity and differentiation of Mongolian and Russian yak populations
Hybridization between wild and domestic bovine species occurs worldwide either spontaneously or by organized crossing. We have analysed hybridization of banteng (Bos javanicus) and zebu (Bos indicus) in south-east Asian cattle using mitochondrial DNA (PCR-RFLP and sequencing), AFLP, satellite fr...
Nijman, I.J.
,
Otsen, M.
,
Verkaar, E.L.C.
,
Ruijter, C. de
,
Hanekamp, E.
,
Ochieng, J.W.
,
Shamshad, S.
,
Rege, J.E.O.
,
Hanotte, Olivier H.
,
Barwegen, M.W.
,
Sulawati, T.
,
Lenstra, J.A.
,
[Hybridization of Banteng (Bos javanicus) and Zebu (Bos indicus) revealed by mitochondrial DNA, satellite DNA, AFLP and microsatellites]
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Hybridization of Banteng (Bos javanicus) and Zebu (Bos indicus) revealed by mitochondrial DNA, satellite DNA, AFLP and microsatellites
With some 180 million head. comprising more than 150 different breeds or populations of taurine Bos taurus, indicine B. indicus and intermediate origin, the African continent is home to an enormous reservoir of cattle biodiversity. Recent archaeological findings are providing new insights into th...
Hanotte, Olivier H.
,
Rege, J.E.O.
,
[Origins, history and relationships of indigenous African cattle]
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Origins, history and relationships of indigenous African cattle
Genetic diversity of sub-Saharan African goats was assessed using 19 microsatellite markers. Breeds were sampled from eastern Africa (Maasai, Kigezi, Mubende, North West Highland, Arsi Bale), southern Africa (Ndebele, Pafuri) and West Africa (West African Dwarf, Maure, Djallonke). European breeds...
Chenyambuga, S.W.
,
Hanotte, Olivier H.
,
Hirbo, J.
,
Watts, P.C.
,
Kemp, Stephen J.
,
Kifaro, G.C.
,
Gwakisa, P.S.
,
Petersen, P.H.
,
Rege, J.E.O.
,
[Genetic characterization of indigenous goats of sub-Saharan Africa using microsatellite DNA markers]
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Genetic characterization of indigenous goats of sub-Saharan Africa using microsatellite DNA markers
Twenty new world camelidae primer pairs were selected for cross-species amplification of loci in the old world camelids, the dromedary (Camelus dromedarius) and the bacterian (camelus bactrianus). A panel of DNA samples from 34 unrelated dromedaries (Kenya) and from 34 unrelated bactrians (32 dom...
Han Jianlin
,
Mburu, D.
,
Ochieng, J.
,
Kaufmann, J.B.
,
Rege, O.
,
Hanotte, Olivier H.
,
[Application of new world camelidae microsatellite primers for amplification of polymorphic loci in old world camelids]
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Application of new world camelidae microsatellite primers for amplification of polymorphic loci in old world camelids
DNA samples from 30 Friesian, 18 N'Dama, 30 Sahiwal and 18 Barotse (derived from Bos taurus and B. indicus) cattle were amplified by the polymerase chain reaction using previously described primer sequences for amplification of the Y chromosome locus INRA124. A polymorphism with 2 alleles was det...
Hanotte, Olivier H.
,
Okomo, M.
,
Verjee, Y.
,
Rege, J.E.O.
,
Teale, A.J.
,
[A polymorphic Y chromosome microsatellite locus in cattle]
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A polymorphic Y chromosome microsatellite locus in cattle
The genetic diversity and relationships amongst the dromedary (Camelus dromedarius) populations are poorly documented. Four recognized Kenyan dromedary breeds (Somali, Turkana, Rendille, Gabbra) and dromedary from Pakistan and the Arabian Peninsula (Saudi Arabia, United Arab Emirates) were studi...
Mburu, D.N.
,
Ochieng, J.W.
,
Kuria, S.G.
,
Han Jianlin
,
Kaufmann, B.
,
Rege, J.E.O.
,
Hanotte, Olivier H.
,
[Genetic diversity and relationships of indigenous Kenyan camel (Camelus dromedarius) populations: Implications for their classification]
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Genetic diversity and relationships of indigenous Kenyan camel (Camelus dromedarius) populations: Implications for their classification
Microsatellite loci are the markers of choice in pedigree analysis and population genetics studies. In closely related species, cross-species amplification of loci may be used instead of the cloning of loci. Previously, successful cross-species amplification of a set of cattle (Bos taurus) micros...
Hanotte, Olivier H.
,
Verjee, Y.
,
Ochieng, J.
,
Rege, J.E.O.
,
[Cattle microsatellite markers for amplification of polymorphic loci in Asian bovidae]
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Cattle microsatellite markers for amplification of polymorphic loci in Asian bovidae
Seven cattle populations indigenous to East Africa and three reference breeds (Sahiwal, N'Dama and Friesian) were characterized with 18 autosomal microsatellite loci. Within-breed diversity was high in all breeds with observed heterozygosities ranging from 0.511 (+ or -) 0.214 (N'Dama) to 0.660 (...
Okomo, M.A.
,
Rege, J.E.O.
,
Teale, A.J.
,
Hanotte, Olivier H.
,
[Genetic characterization of indigenous East African cattle breeds using microsatellite DNA markers]
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Genetic characterization of indigenous East African cattle breeds using microsatellite DNA markers