The paper first describes interleukin-12 P35 encoding gene and discusses its role. It then looks into PCR conditions and sequencing; genotyping; sequencing; polymorphism frequency; mendelian inheritance; chromosomal location in sheep and cattle of different species.
Schmidt, P.
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Kuhn, C.
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Kang'a, S.
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Hanotte, Olivier H.
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Vanselow, J.
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Anton, I.
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Langner, Ch
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Schwerin, M.
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[Interleukin - 12 p35 encoding gene of cattle and sheep harbours a polymorphic T stretch in intron 4]
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Interleukin - 12 p35 encoding gene of cattle and sheep harbours a polymorphic T stretch in intron 4
We describe satellite DNA variation that detects hybridization of Bos indicus (Zebu or indicine cattle) and Bos taurus (taurine cattle) in African cattle populations. On southern blots hybridized to a satellite III probe, relative intensities of Hinf1 fragments correlated with the taurine-zebu co...
Nijman, I.J.
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Bradley, D.G.
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Hanotte, Olivier H.
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Otsen, M.
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Lenstra, J.A.
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[Satellite DNa polymorphisms and AFLP correlate with Bos indicus-taurus hybridization]
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Satellite DNa polymorphisms and AFLP correlate with Bos indicus-taurus hybridization
Twenty new world camelidae primer pairs were selected for cross-species amplification of loci in the old world camelids, the dromedary (Camelus dromedarius) and the bacterian (camelus bactrianus). A panel of DNA samples from 34 unrelated dromedaries (Kenya) and from 34 unrelated bactrians (32 dom...
Han Jianlin
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Mburu, D.
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Ochieng, J.
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Kaufmann, J.B.
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Rege, O.
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Hanotte, Olivier H.
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[Application of new world camelidae microsatellite primers for amplification of polymorphic loci in old world camelids]
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Application of new world camelidae microsatellite primers for amplification of polymorphic loci in old world camelids
The applicability of bovine autosomal micro-satellite markers for population genetic studies on African buffalo was investigated. A total of 168 microsatellite markers were tested for PCR amplification on a test panel of seven African buffalo. Amplification was observed for 139 markers (83 percen...
Hooft, W.F. van
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Hanotte, Olivier H.
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Wenink, P.W.
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Groen, A.F.
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Sugimoto, Y.
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Prins, H.H.T.
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Teale, A.J.
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[Applicability of bovine microsatellite markers for population genetic studies on African buffalo (Syncerus caffer)]
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Applicability of bovine microsatellite markers for population genetic studies on African buffalo (Syncerus caffer)
Information is presented on the frequency of the Msp I (-) allele in the third intron of the bovine growth hormone gene in a large number of cattle breeds. Consideration of the breed frequencies in relation to their geographic origin shows a low frequency for breeds originating in Northern Europe...
Lagziel, A.S.
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DeNise, S.
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Hanotte, Olivier H.
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Dhara, S.
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Glazko, V.
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Broadhead, A.
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Davoli, R.
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Russo, V.
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Soller, M.
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[Geographic and breed distribution of an Msp I PCR-RFLP in the bovine growth hormone (bGH) gene]
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Geographic and breed distribution of an Msp I PCR-RFLP in the bovine growth hormone (bGH) gene
DNA samples from 30 Friesian, 18 N'Dama, 30 Sahiwal and 18 Barotse (derived from Bos taurus and B. indicus) cattle were amplified by the polymerase chain reaction using previously described primer sequences for amplification of the Y chromosome locus INRA124. A polymorphism with 2 alleles was det...
Hanotte, Olivier H.
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Okomo, M.
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Verjee, Y.
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Rege, J.E.O.
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Teale, A.J.
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[A polymorphic Y chromosome microsatellite locus in cattle]
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A polymorphic Y chromosome microsatellite locus in cattle
Hanotte, Olivier H.
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Verjee, Y.
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Ochieng, O.
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Teale, A.J.
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Rege, J.E.O.
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[Usefulness of cattle microsatellite markers for amplification of polymorphic loci in Asian bovidae]
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Usefulness of cattle microsatellite markers for amplification of polymorphic loci in Asian bovidae
Microsatellite loci are the markers of choice in pedigree analysis and population genetics studies. In closely related species, cross-species amplification of loci may be used instead of the cloning of loci. Previously, successful cross-species amplification of a set of cattle (Bos taurus) micros...
Hanotte, Olivier H.
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Verjee, Y.
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Ochieng, J.
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Rege, J.E.O.
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[Cattle microsatellite markers for amplification of polymorphic loci in Asian bovidae]
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Cattle microsatellite markers for amplification of polymorphic loci in Asian bovidae
Microsatellite loci are tandem repeats of short DNA sequences (1-5bp). Polymorphism in these loci results from variation in the number of repeat units. Microsatellites are numerous and dispersed among the chromosomes in all eukaryotic genomes. They are now the markers of choice for genome mapping...
Hishida, O.
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Hanotte, Olivier H.
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Verjee, Y.
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Tanka, K.
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Namikawa, T.
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Teale, A.J.
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Rege, J.E.O.
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[Cross-species amplification and polymorphism of microsatellite loci in Asian bovidae]
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Cross-species amplification and polymorphism of microsatellite loci in Asian bovidae