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An alternative approach for the direct analysis of chromosome regions corresponding to economical traits, based on chromosome microdissection, is described. Large fragment clones isolated with primer pairs designed from chromosome fragment-specific DNA sequences were localized by fluorescence in ...


Goldammer, T.Brunner, R.M.Kang'a, S.Hanotte, Olivier H.Schwerin, M.[Establishment of bovine chromosome region specific high resolution maps using microdissection]Establishment of bovine chromosome region specific high resolution maps using microdissection

With some 180 million head. comprising more than 150 different breeds or populations of taurine Bos taurus, indicine B. indicus and intermediate origin, the African continent is home to an enormous reservoir of cattle biodiversity. Recent archaeological findings are providing new insights into th...


Hanotte, Olivier H.Rege, J.E.O.[Origins, history and relationships of indigenous African cattle]Origins, history and relationships of indigenous African cattle

Random amplified polymorphic DNA (RAPD) variation is being used increasingly to characterize germplasm collections. However, the technique has limitations due to the lack of reproducibility of results under certain conditions. Here we review some of the literature on the factors that are mainly r...


Lowe, A.J.Hanotte, Olivier H.Guarino, Luigi[Standardization of molecular genetic techniques for the characterization of germplasm collections: The case of random amplified polymorphic DNA (RAPD)]Standardization of molecular genetic techniques for the characterization of germplasm collections: The case of random amplified polymorphic DNA (RAPD)

Genetic diversity of sub-Saharan African goats was assessed using 19 microsatellite markers. Breeds were sampled from eastern Africa (Maasai, Kigezi, Mubende, North West Highland, Arsi Bale), southern Africa (Ndebele, Pafuri) and West Africa (West African Dwarf, Maure, Djallonke). European breeds...


Chenyambuga, S.W.Hanotte, Olivier H.Hirbo, J.Watts, P.C.Kemp, Stephen J.Kifaro, G.C.Gwakisa, P.S.Petersen, P.H.Rege, J.E.O.[Genetic characterization of indigenous goats of sub-Saharan Africa using microsatellite DNA markers]Genetic characterization of indigenous goats of sub-Saharan Africa using microsatellite DNA markers

We describe satellite DNA variation that detects hybridization of Bos indicus (Zebu or indicine cattle) and Bos taurus (taurine cattle) in African cattle populations. On southern blots hybridized to a satellite III probe, relative intensities of Hinf1 fragments correlated with the taurine-zebu co...


Nijman, I.J.Bradley, D.G.Hanotte, Olivier H.Otsen, M.Lenstra, J.A.[Satellite DNa polymorphisms and AFLP correlate with Bos indicus-taurus hybridization]Satellite DNa polymorphisms and AFLP correlate with Bos indicus-taurus hybridization

Twenty new world camelidae primer pairs were selected for cross-species amplification of loci in the old world camelids, the dromedary (Camelus dromedarius) and the bacterian (camelus bactrianus). A panel of DNA samples from 34 unrelated dromedaries (Kenya) and from 34 unrelated bactrians (32 dom...


Han JianlinMburu, D.Ochieng, J.Kaufmann, J.B.Rege, O.Hanotte, Olivier H.[Application of new world camelidae microsatellite primers for amplification of polymorphic loci in old world camelids]Application of new world camelidae microsatellite primers for amplification of polymorphic loci in old world camelids
Chenyambuga, S.W.Watt, P.C.Hirbo, J.Guakisa, P.S.Kifaro, G.C.Kemp, Stephen J.Hanotte, Olivier H.Rege, J.E.O.[Assessment of genetic diversity of East African goat populations using microsatellite DNA markers]Assessment of genetic diversity of East African goat populations using microsatellite DNA markers

Genetic markers are being used by scientists worldwide in both animal and plant related breeding and Development efforts. The International Livestock Research Institute (ILRI) is a new institute formed in January 1995 by the amalgamation of the former ILCA (International Livestock Centre for Afri...


Tarawali, Shirley A.Rege, J.E.O.Hanotte, Olivier H.Hanson, Jean[DNA markers for forage and livestock improvement]DNA markers for forage and livestock improvement

Dromedaries have been fundamental to the development of human societies in arid landscapes and for long-distance trade across hostile hot terrains for 3,000 y. Today they continue to be an important livestock resource in marginal agro-ecological zones. However, the history of dromedary domesticat...


Almathen, F.Charruau, P.Mohandesan, E.Mwacharo, Joram M.Orozco-terWengel, P.Pitt, D.Abdussamad, A.M.Uerpmann, M.Uerpmann, H.P.Cupere, B. deMagee, P.Alnaqeeb, M.A.Salim, B.Raziq, A.Dessie, TadelleAbdelhadi, O.M.Banabazi, M.H.Al-Eknah, M.Walzer, C.Faye, B.Hofreiter, M.Peters, J.Hanotte, Olivier H.Burger, P.A.[Ancient and modern DNA reveal dynamics of domestication and cross-continental dispersal of the dromedary]Ancient and modern DNA reveal dynamics of domestication and cross-continental dispersal of the dromedary

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