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Microsatellite amplification was performed on cassava (Manihot esculenta) and six other different species (all wild) of the Manihot genus. We used ten pairs of microsatellite primers previously developed from cassava, detecting 124 alleles in a sample of 121 accessions of the seven species. The n...


Roa, ACChavarriaga Aguirre, PaulDuque E., Myriam CristinaMaya, M.M.Bonierbale, Merideth W.Iglesias, C.A.Tohme, Joseph M.[Cross-species amplification of cassava (Manihot esculenta) (Euphorbiaceae) microsatellites: Allelic polymorphism and degree of relationship]Cross-species amplification of cassava (Manihot esculenta) (Euphorbiaceae) microsatellites: Allelic polymorphism and degree of relationship

People in vast areas of the tropics suffer from vitamin A deficiency, resulting in progressive eye damage and eventually leading to blindness. Improving the content of vitamin A precursors in staple crops could alleviate or solve such a problem. The objective of this work was to study the range o...


Iglesias, C.A.Mayer, Jorge EdgardChavez, L.Calle, F[Genetic potential and stability of carotene content in cassava roots]Genetic potential and stability of carotene content in cassava roots

The cassava core collection (601 genotypes) was evaluated for root and leaf contents of micronutrient minerals, ascorbic acid, and carotene. Wide genetic variability was observed for all measurements, indicating that there is good potential for exploiting and improving the nutritive value of cass...


Chavez, Alba L.Bedoya Flores, JMSánchez, T.Iglesias, C.A.Ceballos, H.Roca, William M.[Iron, carotene, and ascorbic acid in cassava roots and leaves]Iron, carotene, and ascorbic acid in cassava roots and leaves

The gene pools for breeding cassava (Manihot esculenta Crantz) in Africa currently contain only a fraction of the existing genetic variation found in Latin America where the crop originates. Our research aimed to broaden the genetic base in Africa by introducing Latin American (LA) germplasm. The...


Okogbenin, EmmanuelPorto, M.C.M.Egesi, Chiedozie N.Mba, CEspinosa, E.Santos Melendez, LGOspina, C.Marin, JABarrera Sabogal, EGutiérrez A., Janeth PatriciaEkanayake, I.J.Iglesias, C.A.Fregene, Martin A.[Marker-assisted introgression of resistance to cassava mosaic disease into Latin American germplasm for the genetic improvement of cassava in Africa]Marker-assisted introgression of resistance to cassava mosaic disease into Latin American germplasm for the genetic improvement of cassava in Africa

Commercial cassava production from true cassava seeds (TCS) appears to be a promising option for reducing or eliminating several of the production constraints associated with vegetative propagation. The most important contribution of TCS would be to reduce virus build-up in vegetative material an...


Iglesias, C.A.Hershey, Clair H.Calle, FBolaños, A.[Propagating cassava (Manihot esculenta) by sexual seed]Propagating cassava (Manihot esculenta) by sexual seed

The genome locations of the wound-response genes that were expressedduring the post-harvest physiological deterioration (PPD) of cassava, suchas phenylalanine ammonia lyase, ?-1.3 glucanase, hydroxyprolinerich glycoprotein, catalase, 1-aminocyclopropane 1-carboxylate, cysteineprotease inhibitor, ...


Cortés Bermúdez, D.F.Reilly, KOkogbenin, EmmanuelBeeching, JRIglesias, C.A.Tohme, Joseph M.[Mapping wound-response genes involved in post-harvest physiological deterioration (PPD) of cassava (Manihot esculenta Crantz)]Mapping wound-response genes involved in post-harvest physiological deterioration (PPD) of cassava (Manihot esculenta Crantz)

The cassava core collection was selected to represent, with minimum repetitiveness, the potential genetic diversity of the crop. The core (630 accessions) was chosen from the base collection (over 5500 accessions) on the basis of diversity of origin (country and geographic), morphology, isozyme p...


Chavarriaga Aguirre, PaulMaya, M.M.Tohme, Joseph M.Duque E., Myriam CristinaIglesias, C.A.Bonierbale, Merideth W.Kresovich, SKochert, G[Using microsatellites, isozymes and AFLPs to evaluate genetic diversity and redundancy in the cassava core collection and to assess the usefulness of DNA-BASED markers to maintain germplasm collections]Using microsatellites, isozymes and AFLPs to evaluate genetic diversity and redundancy in the cassava core collection and to assess the usefulness of DNA-BASED markers to maintain germplasm collections

About 70 million people obtain more than 500 cal per day from cassava roots. The crop is fundamental as food security of poor rural communities, but little is known about variability of root nutritional and quality traits. Roots from 2457 genotypes comprising landraces and improved clones, were s...


Chavez, Alba L.Sánchez, T.Jaramillo, GustavoBedoya Flores, JMEcheverry, JBolaños Vidal, E.A.Ceballos, H.Iglesias, C.A.[Variation of quality traits in cassava roots evaluated in landraces and improved clones]Variation of quality traits in cassava roots evaluated in landraces and improved clones

Although cassava is a major food crop, its scientific breeding began only recently compared with other crops. Significant progress has been achieved, particularly in Asia where cassava is used mainly for industrial processes and no major biotic constraints affect its productivity. Cassava breedin...


Ceballos, H.Iglesias, C.A.Pérez Velásquez, Juan CarlosDixon, Alfred G.O.[Cassava breeding: opportunities and challenges]Cassava breeding: opportunities and challenges

Cassava (Manihot esculenta Crantz) is a tough and productive survival crop of smallholder farmers in the tropics. It is often grown where other crops fail. Research can make important contribution to food security and economic development in cassava-growing areas. This can be enhanced through in-...


Thro, Ann MarieRoca, William M.Iglesias, C.A.Henry, GuyNg, S.Y.C.[Contributions of in-vitro biology to cassava improvement]Contributions of in-vitro biology to cassava improvement

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