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In vitro culture media capable of regenerating moderate to high shoots and/or plantlets from meristems of two yam species – Dioscorea rotundata and Dioscorea alata within comparable duration of 10 weeks as commonly obtained in other monocots and root and tuber crops were investigated. The study c...


Adeniyi, O.J.Adetimirin, V.O.Ingelbrecht, I.L.Asiedu, Robert[Shoot and plantlet regeneration from meristems of Dioscorea rotundata poir and Dioscorea alata L.]Shoot and plantlet regeneration from meristems of Dioscorea rotundata poir and Dioscorea alata L.

Cassava (Manihot esculenta Crantz) is a staple food for over 600 million people in the tropics and sub-tropics and is increasingly used as an industrial crop for starch production. Cassava has a high growth rate under optimal conditions but also performs well in drought-prone areas and on margina...


Lokko, Y.Anderson, J.Rudd, S.Raji, A.Horvath, D.Mikel, M.A.Kim, R.Liu, L.Hernández, A.Dixon, A.Ingelbrecht, I.L.[Characterization of an 18,166 EST dataset for cassava (Manihot esculenta Crantz) enriched for drought responsive genes]Characterization of an 18,166 EST dataset for cassava (Manihot esculenta Crantz) enriched for drought responsive genes

A study was conducted to investigate the genetic variability between 40 Musa genotypes maintained at the Musa germplasm collection of the International Institute for Tropical Agriculture, Ibadan using nine B-genome derived simple sequence repeat (SSR) markers. The nine primers produced reproducib...


Oriero, C.E.Odunola, O.A.Lokko, Y.Ingelbrecht, I.L.[Analysis of Bgenome derived simple sequence repeat (SSR) markers in Musa spp.]Analysis of Bgenome derived simple sequence repeat (SSR) markers in Musa spp.

The experiment was conducted to test the in vitro regeneration efficiency of cowpea shoots via embryonic axes explants and the effect of benzyl amino purine (BAP) concentration on shoot formation. A total of 600 explants (embryonic axes) were plated from which 237 shoots were regenerated. The ave...


Yusuf, M.Raji, A.A.Ingelbrecht, I.L.Katung, M.D.[Regeneration efficiency of cowpea [Vigna unguiculata (L.) Walp.] via embryonic axes explants]Regeneration efficiency of cowpea [Vigna unguiculata (L.) Walp.] via embryonic axes explants

A rapid and reproducible in vitro plant regeneration procedure was developed for embryonic axis explants of cowpea [Vigna unguiculata (L.) Walp]. Moderate levels of 6-benzylaminopurine (0.5-2 mg l-1) were effective in inducing multiple shoots on decapitated embryonic axes on a Murashige and Skoog...


Raji, A.A.Oriero, E.Odeseye, B.Odunlami, T.Ingelbrecht, I.L.[Plant regeneration and Agrobacteriummediated transformation of African cowpea (Vigna unguiculata (L.) Walp) genotypes using embryonic axis explants]Plant regeneration and Agrobacteriummediated transformation of African cowpea (Vigna unguiculata (L.) Walp) genotypes using embryonic axis explants

Some genetic studies using molecular methods such as diversity assessment or marker-assisted selection require collection of a large number of samples from fields located in the vicinity or in remote areas, followed by isolation of good quality DNA in a short time span. In the present study, diff...


Bhattacharjee, R.Ferguson, M.Gedil, M.Dumet, D.Ingelbrecht, I.L.[Field collection,preservation and large scale DNA extraction procedures for cassava (Manihot esculenta Crantz.)]Field collection,preservation and large scale DNA extraction procedures for cassava (Manihot esculenta Crantz.)

The study described a T-DNA vectorwith a Cassava veinmosaic virus promoter cassette (pCsVMV) and a kanamycin selectable marker gene driven by the 35S Cauliflower mosaic virus promoter with a view to stably express transgenes over repeated cycles of clonal propagation. A ?-glucuronidase reporter g...


Oyelakin, O.O.Opabode, J.T.Raji, A.A.Ingelbrecht, I.L.[A Cassava vein mosaic virus promoter cassette induces high and stable gene expression in clonally propagated transgenic cassava (Manihot esculenta Crantz)]A Cassava vein mosaic virus promoter cassette induces high and stable gene expression in clonally propagated transgenic cassava (Manihot esculenta Crantz)

A morphological diversity study was conducted on African rice gall midge Orseolia oryzivora Harris & Gagné and two other African species, Orseolia bonzii Harris and Orseolia nwanzei Harris & Nwilene, sp. n. Morphological differences between adult and immature stages of the three species are sligh...

Une etude de la djversite morphologique a ete reaHsee sur la cecidomyie de la galle du riz africain, Orseolia oryzivora Harris & Gagne et sur deux autres especes africaines, O. bonzii Harris et O. nwallzei Harris & Nwilene, sp. n. Les difference morphologiques entre les stades adultes et immature...


Nwilene, F.E.Harris, K.M.Okhidievbie, O.Onasanya, A.Sere, Y.Ingelbrecht, I.L.[Morphological diversity and genomic DNA fingerprinting of the African rice gall midge Orseolia oryzivora (Diptera: Cecidomyiidae) and of two other species of African Orseolia]Morphological diversity and genomic DNA fingerprinting of the African rice gall midge Orseolia oryzivora (Diptera: Cecidomyiidae) and of two other species of African Orseolia

Theobroma cacao L. with its center of diversity in Central and South America was first introduced to West Africa in the mid-19th century and today the region produces 70% of the world's cocoa. Several distinct cocoa types have been introduced, cultivated, and intercrossed across the region. Also,...


Opoku, S.Bhattacharjee, R.Kolesnikova-Allen, M.Motamayor, J.Schnell, R.Ingelbrecht, I.L.Enu-Kwesi, L.Adu-Ampomah, Y.[Genetic diversity in cocoa (Theobroma cacao L.) germplasm collection from Ghana]Genetic diversity in cocoa (Theobroma cacao L.) germplasm collection from Ghana

Introduction of exotic maize (Zea mays L.) into adapted tropical germplasm may enhance genetic variability and lead to greater progress from selection. The first objective of this study was to determine if yellow endosperm lines derived from adapted x exotic backcrosses contain exotic alleles tha...


Menkir, A.Olowolafe, M.O.Ingelbrecht, I.L.Fawole, I.Badu-Apraku, B.Vroh Bi, Irie[Assessment of testcross performance and genetic diversity of yellow endosperm maize lines derived from adapted x exotic backcrosses]Assessment of testcross performance and genetic diversity of yellow endosperm maize lines derived from adapted x exotic backcrosses

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