Two bovine bacterial artificial chromosome clones were screened by PCR to amplify the tumour necrosis factor alpha (TNFA) gene. DNA extraction and Southern blotting and probing with a (GT)10 oligonucleotide were performed, followed by cloning into the pUC19 vector. A 0.9 kb fragment containing se...
Wambugu, J.
,
Kanguha, E.
,
Hanotte, Olivier H.
,
Davis, S.
,
Taylor, J.
,
Skow, L.
,
Brunner, R.
,
Schwerin, M.
,
Teale, A.J.
,
Iraqi, F.A.
,
[Identification of two polymorphic microsatellite repeats on a bovine TNFA-containing BAC clone]
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Identification of two polymorphic microsatellite repeats on a bovine TNFA-containing BAC clone
The host-protective immune response to infection with gastrointestinal (GI) nematodes involves a range of interacting processes that begin with recognition of the parasite's antigens and culminate in an inflammatory reaction in the intestinal mucosa. Precisely which immune effectors are responsib...
Behnke, J.M.
,
Iraqi, F.A.
,
Menge, D.
,
Baker, R.L.
,
Gibson, John P.
,
Wakelin, D.
,
[Chasing the genes that control resistance to gastrointestinal nematodes]
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Chasing the genes that control resistance to gastrointestinal nematodes
Since the publication of the mouse genome, attention has focused on the strains that were selected for sequencing. In this paper we report the results of experiments that characterized the response to infection with the murine gastrointestinal nematode Heligmosomoides polygyrus of eight new strai...
Behnke, J.M.
,
Mugambi, J.M.M.
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Clifford, S.
,
Iraqi, F.A.
,
Baker, R.L.
,
Gibson, John P.
,
Wakelin, D.
,
[Genetic variation in resistance to repeated infections with Heligmosomoides polygyrus bakeri, in inbred mouse strains selected for the mouse genome project]
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Genetic variation in resistance to repeated infections with Heligmosomoides polygyrus bakeri, in inbred mouse strains selected for the mouse genome project
Quantitative trait loci (QTL) associated with resistance to an intestinal worm in a well-defined murine model are described. These have been identified in an F(2) population derived from resistant (SWR) and susceptible (CBA) parental mouse strains infected with the gastro-intestinal nematode par...
We developed an F(11) AIL population from an F(1) cross of A/J (susceptible) and C57BL/6J (resistant) mouse strains. One thousand F(11) mice were challenged with P.c. chabaudi 54X, and 340 mice selected from the phenotypic extremes for susceptibility and resistance were genotyped for microsatel...
Hernández Valladares, M.
,
Naessens, Jan
,
Gibson, John P.
,
Musoke, A.J.
,
Nagda, S.M.
,
Rihet, P.
,
Ole-MoiYoi, O.K.
,
Iraqi, F.A.
,
[Confirmation and dissection of QTL controlling resistance to malaria in mice]
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Confirmation and dissection of QTL controlling resistance to malaria in mice
The tumor necrosis factor (TNF) locus comprises the tandemly arranged genes coding for Tnfa (cachectin)and Tnfb (lymphotoxin-alpha) (Semon et al. 1987). In mice, the TNF locus is located on chromosome 17 (MMU17) in the major histocompatibility complex (MHC)region. Tnfa and soluble lymphotoxin alp...
Iraqi, F.A.
,
Teale, A.J.
,
[Polymorphisms in the TNFa but not in TNFb microsatellite and correlation with trypanosomiasis in mice]
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Polymorphisms in the TNFa but not in TNFb microsatellite and correlation with trypanosomiasis in mice