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Moreta, DaniloArango, JacoboSotelo Cabrera, Mauricio EfrenVergara, DanielRincón, ÁlvaroIshitani, ManabuCastro, AracelyMiles, John W.Peters, MichaelTohme, Joseph M.Subbaraoa, Guntur V.Rao, Idupulapati M.[Biological nitrification inhibition (BNI) in Brachiaria pastures: a novel strategy to improve eco-efficiency of crop-livestock systems and to mitigate climate change]Biological nitrification inhibition (BNI) in Brachiaria pastures: a novel strategy to improve eco-efficiency of crop-livestock systems and to mitigate climate change

We evaluated the yields of Oryza sativa L. ‘Nipponbare’ rice lines expressing a gene encoding an A20/AN1 domain stress-associated protein, AlSAP, from the halophyte grass Aeluropus littoralis under the control of different promoters. Three independent field trials were conducted, with drought imp...


Ghneim-Herrera, ThauraSelvaraj, Michael GomezMeynard, DonaldoFabre, DenisPeña, AlexandraRomdhane, Walid BenSaad, Rania BenOgawa, SatoshiRebolledo, María CamilaIshitani, ManabuTohme, Joseph M.Al-Doss, AbdullahGuiderdoni, EmmanuelHassairi, Afif[Expression of the Aeluropus littoralis AlSAP Gene Enhances Rice Yield under Field Drought at the Reproductive Stage]Expression of the Aeluropus littoralis AlSAP Gene Enhances Rice Yield under Field Drought at the Reproductive Stage

Nitrogen (N) fertilizers are a major input cost in rice production, and its excess application leads to major environmental pollution. Development of rice varieties with improved nitrogen use efficiency (NUE) is essential for sustainable agriculture. Here, we report the results of field evaluatio...


Selvaraj, Michael GomezValencia, Milton OrlandoOgawa, SatoshiLu, YingzhiWu, LiyingDowns, ChristopherSkinner, WayneLu, ZhongjinKridl, Jean C.Ishitani, ManabuBoxtel, Jos van[Development and field performance of nitrogen use efficient rice lines for Africa]Development and field performance of nitrogen use efficient rice lines for Africa

The apomictic reproductive mode of Brachiaria (syn. Urochloa) forage species allows breeders to faithfully propagate heterozygous genotypes through seed over multiple generations. In Brachiaria, reproductive mode segregates as single dominant locus, the apospory-specific genomic region (ASGR). Th...


Worthington, MargaretEbina, MasumiYamanaka, NaokiHeffelfinger, ChristopherQuintero, ConstanzaZapata, Yeny PatriciaPérez, Juan GuillermoSelvaraj, MichaelIshitani, ManabuDuitama, JorgeHoz, Juan Fernando de laRao, Idupulapati M.Dellaporta, Stephen L.Tohme, Joseph M.Arango, Jacobo[Translocation of a parthenogenesis gene candidate to an alternate carrier chromosome in apomictic Brachiaria humidicola]Translocation of a parthenogenesis gene candidate to an alternate carrier chromosome in apomictic Brachiaria humidicola
Arango, JacoboMoreta, D.Núñez, J.Hartmann, K.Domínguez, M.Ishitani, ManabuMiles, John W.Subbaraoa, Guntur V.Peters, MichaelRao, Idupulapati M.[Developing methods to evaluate phenotypic variability in biological nitrification inhibition (BNI) capacity of Brachiaria grasses]Developing methods to evaluate phenotypic variability in biological nitrification inhibition (BNI) capacity of Brachiaria grasses
Ogawa, SatoshiValencia, Milton OrlandoLorieux, MathiasArbeláez Vélez, Juan D.McCouch, Susan R.Ishitani, ManabuGómez Selvaraj, Michael[Identification of QTLs associated with agronomic performance under nitrogen-deficient conditions using chromosome segment substitution lines of a wild rice relative, Oryza rufipogon]Identification of QTLs associated with agronomic performance under nitrogen-deficient conditions using chromosome segment substitution lines of a wild rice relative, Oryza rufipogon

We developed and modified a precise, rapid, and reproducible protocol isolating high‐quality RNA from tissues of multiple varieties of cassava plants (Manihot esculenta Crantz). The resulting method is suitable for use in mini, midi, and maxi preparations and rapidly achieves high total RNA yield...


Behnam, BabakBohórquez Cháux, AdrianaCastañeda-Méndez, Oscar F.Tsuji, HiroyukiIshitani, ManabuBecerra López Lavelle, Luis Augusto[An optimized isolation protocol yields high‐quality RNA from cassava tissues (Manihot esculenta Crantz)]An optimized isolation protocol yields high‐quality RNA from cassava tissues (Manihot esculenta Crantz)
Rao, Idupulapati M.Ishitani, ManabuMiles, John W.Peters, MichaelTohme, Joseph M.Arango, JacoboMoreta, Danilo ELópez, HernánCastro, AracelyHoek, Rein van derMartens, Siriwan D.Hyman, GlennTapasco, JeimarDuitama, JorgeSuárez Baron, HaroldBorrero, GonzaloNúñez, JonathanHartmann, KatharinaDomínguez, MoralbaSotelo Cabrera, Mauricio EfrenVergara, DanielLavelle, PatrickSubbaraoa, Guntur V.Rincón, ÁlvaroPlazas, CamiloMendoza, ReynaldoRathjen, LenaKarwat, HannesCadisch, Georg[Climate-smart crop-livestock systems for smallholders in the tropics: integration of new forage hybrids to intensify agriculture and to mitigate climate change through regulation of nitrification in soil]Climate-smart crop-livestock systems for smallholders in the tropics: integration of new forage hybrids to intensify agriculture and to mitigate climate change through regulation of nitrification in soil

Drought stress has often caused significant decreases in crop production which could be associated with global warming. Enhancing drought tolerance without a grain yield penalty has been a great challenge in crop improvement. Here, we report the Arabidopsis thaliana galactinol synthase 2 gene (At...


Selvaraj, Michael GomezIshizaki, TakumaValencia, Milton OrlandoOgawa, SatoshiDedicova, BeataOgata, TakuyaYoshiwara, KyoukoMaruyama, KyonoshinKusano, MiyakoSaito, KazukiTakahashi, FuminoriShinozaki, KazuoNakashima, KazuoIshitani, Manabu[Overexpression of an Arabidopsis thaliana galactinol synthase gene improves drought tolerance in transgenic rice and increased grain yield in the field]Overexpression of an Arabidopsis thaliana galactinol synthase gene improves drought tolerance in transgenic rice and increased grain yield in the field

Application of field based high-throughput phenotyping (FB-HTP) methods for monitoring plant performance in real field conditions has a high potential to accelerate the breeding process. In this paper, we discuss the use of a simple tower based remote sensing platform using modified single-lens r...


Naito, HirokiOgawa, SatoshiValencia, Milton OrlandoMohri, HirokiUrano, YutakaHosoi, FumikiShimizu, YoChavez, Alba L.Ishitani, ManabuSelvaraj, Michael GomezOmasa, Kenji[Estimating rice yield related traits and quantitative trait loci analysis under different nitrogen treatments using a simple tower-based field phenotyping system with modified single-lens reflex cameras]Estimating rice yield related traits and quantitative trait loci analysis under different nitrogen treatments using a simple tower-based field phenotyping system with modified single-lens reflex cameras

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