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We developed and modified a precise, rapid, and reproducible protocol isolating high‐quality RNA from tissues of multiple varieties of cassava plants (Manihot esculenta Crantz). The resulting method is suitable for use in mini, midi, and maxi preparations and rapidly achieves high total RNA yield...


Behnam, BabakBohórquez Cháux, AdrianaCastañeda-Méndez, Oscar F.Tsuji, HiroyukiIshitani, ManabuBecerra López Lavelle, Luis Augusto[An optimized isolation protocol yields high‐quality RNA from cassava tissues (Manihot esculenta Crantz)]An optimized isolation protocol yields high‐quality RNA from cassava tissues (Manihot esculenta Crantz)
Tokunaga, HirokiHai Anh, NguyenDong, Nguyen VanHam, Le HuyHanh, Nguyen ThiHung, NguyenIshitani, ManabuTuan, Le NgocUtsumi, YoshinoriVu, Nguyen AnhSeki, Motoaki[An efficient method of propagating cassava plants using aeroponic culture]An efficient method of propagating cassava plants using aeroponic culture
Utsumi, YoshinoriTanaka, MahoKurotani, AtsushiYoshida, TakuhiroMochida, KeiichiMatsui, AkihiroIshitani, ManabuSraphet, SupajitWhankaew, SukhumanAsvarak, ThipaNarangajavana, JarunyaTriwitayakorn, KanokpornSakurai, TetsuyaSeki, Motoaki[Cassava (Manihot esculenta) transcriptome analysis in response to infection by the fungus Colletotrichum gloeosporioides using an oligonucleotide-DNA microarray]Cassava (Manihot esculenta) transcriptome analysis in response to infection by the fungus Colletotrichum gloeosporioides using an oligonucleotide-DNA microarray

Post-harvest physiological deterioration (PPD) is one of the most important constraints in cassava production and commercialization. It has been hypothesized that the antioxidant properties of carotenoids in yellow cassava roots may help reduce or delay PPD. The industrial sector prefers cassava ...


Sánchez, T.Chavez, Alba L.Ceballos, H.Rodríguez Amaya, D.B.Nestel, PIshitani, Manabu[Reduction or delay of post-harvest physiological deterioration in cassava roots with higher carotenoid content]Reduction or delay of post-harvest physiological deterioration in cassava roots with higher carotenoid content

Background Cassava, an allotetraploid known for its remarkable tolerance to abiotic stresses is an important source of energy for humans and animals and a raw material for many industrial processes. A full-length cDNA library of cassava plants under normal, heat, drought, aluminum and post harves...


Sakurai, TetsuyaPlata, GermanRodriguez, FaustoSeki, MotoaliSalcedo, AndresToyoda, AtsushiHiwata, AtsushiTohme, Joseph M.Sake, YoshiyukiShinozaki, KazuoIshitani, Manabu[Sequencing analysis of 20,000 full-length cDNA clones from cassava reveals lineage specific expansions in gene families related to stress response]Sequencing analysis of 20,000 full-length cDNA clones from cassava reveals lineage specific expansions in gene families related to stress response
Chávez, Alba L.Sánchez, T.Tohme, Joseph M.Ishitani, ManabuCeballos, H.[Effect of processing on B-carotene content of cassava roots]Effect of processing on B-carotene content of cassava roots
Chávez, Alba L.Sánchez, T.Tohme, Joseph M.Ishitani, ManabuCeballos, H.[Sampling variability in cassava roots for total carotene content]Sampling variability in cassava roots for total carotene content
Chávez, Alba L.Ishitani, ManabuSánchez, T.Domínguez García, M.Ceballos, H.[Reliability of freezing carotenoids extracts for an efficient tandem selection of high B-carotene in cassava]Reliability of freezing carotenoids extracts for an efficient tandem selection of high B-carotene in cassava
Utsumi, YTanaka, MMorosawa, TKurotani, AYoshida, TMochida, KMatsui, AUmemura, YIshitani, ManabuShinozaki, KSakurai, TSeki, M[Transcriptome analysis using a high-density oligomicroarray under drought stress in various genotypes of cassava: an important tropical crop]Transcriptome analysis using a high-density oligomicroarray under drought stress in various genotypes of cassava: an important tropical crop

Cassava (Manihot esculenta Crantz) is an important tropical root crop that provides food security and income generation for many farmers. Cassava ranks as the third most important source of calories in the tropics and provides sustenance to 800 million people worldwide. Cassava is also an efficie...


Utsumi, YSakurai, TUmemura, YoshimiAyling, SarahIshitani, ManabuNarangajavana, JSojikul, PTriwitayakorn, KanokpornMatsui, MManabe, RShinozaki, KSeki, M[RIKEN Cassava Initiative: establishment of a cassava functional genomics platform]RIKEN Cassava Initiative: establishment of a cassava functional genomics platform

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