Current serological tests for Babesia bigemina use semipurified merozoite antigens derived from infected erythrocytes. One of the major drawbacks of these tests is that antigen quality can vary from batch to batch. Since the quality of the antigen contributes to the sensitivity and specificity of...
Tebele, N.
,
Skilton, Robert A.
,
Katende, J.
,
Wells, C.W.
,
Nene, Vishvanath
,
McElwain, T.F.
,
Morzaria, S.P.
,
Musoke, A.J.
,
[Cloning, characterization and expression of a 200 Kilodalton diagnostic antigen of Babesia bigemina]
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Cloning, characterization and expression of a 200 Kilodalton diagnostic antigen of Babesia bigemina
Field and experimental bovine infection sera were used in immunoblots of sporozoite and schizont lysates of Theileria parva to identify candidate diagnostic antigens. Four parasite antigens of Mr 67,000 (p67), 85,000 (the polymorphic immunodominant molecule, PIM), 104,000 (p104), and 150,000 (p15...
Katende, J.
,
Morzaria, S.P.
,
Toye, Philip G.
,
Skilton, Robert A.
,
Nene, Vishvanath
,
Nkonge, C.
,
Musoke, A.J.
,
[An enzyme-linked immunosorbent assay for detection of Theileria parva antibodies in cattle using a recombinant polymorphic immunodominant molecule]
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An enzyme-linked immunosorbent assay for detection of Theileria parva antibodies in cattle using a recombinant polymorphic immunodominant molecule
Two recombinant vaccines against Theileria parva, based on a near full-length version of the sporozoite surface antigen p67 (p67635), or an 80 amino acid C-terminal section (p67C), were evaluated by exposure of immunized cattle to natural tick challenge in two sites at the Kenya Coast and one i...
Musoke, A.J.
,
Rowlands, G.J.
,
Nene, Vishvanath
,
Nyanjui, J.
,
Katende, J.
,
Spooner, P.R.
,
Mwaura, S.
,
Odongo, D.
,
Nkonge, C.
,
Mbogo, S.
,
Bishop, Richard P.
,
Morzaria, S.P.
,
[Subunit vaccine based on the p67 major surface protein of Theileria parva sporozoites reduces severity of infection derived from field tick challenge]
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Subunit vaccine based on the p67 major surface protein of Theileria parva sporozoites reduces severity of infection derived from field tick challenge