Microarrays are an important and widely used tool. Applications include capturing genomic DNA for high-throughput sequencing in addition to the traditional monitoring of gene expression and identifying DNA copy number variations. Sequence mismatches between probe and target strands are known to a...
Rennie, C.
,
Noyes, H.A.
,
Kemp, Stephen J.
,
Hulme, H.
,
Brass, A.
,
Hoyle, D.C.
,
[Strong position-dependent effects of sequence mismatches on signal ratios measured using long oligonucleotide microarrays]
,
Strong position-dependent effects of sequence mismatches on signal ratios measured using long oligonucleotide microarrays
To assess the level and spatial pattern of genetic diversity of the common dormouse, Muscardinus avellanarius, we developed polymorphic microsatellite loci from partial genomic libraries enriched for microsatellite motifs. Ten dinucleotide polymorphic microsatellites were isolated and levels of g...
Naim, D.M.D.
,
Kemp, Stephen J.
,
Telfer, S.
,
Watts, P.C.
,
[Isolation and characterization of 10 microsatellite loci in the common dormouse Muscardinus avellanarius]
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Isolation and characterization of 10 microsatellite loci in the common dormouse Muscardinus avellanarius
Conventional disciplinary research approach is losing momentum in the face of dynamic health challenges of the 21st
century. There is a need for a new, suitable approach, to tackle these emerging and re-emerging human and animal
diseases through integrating livestock and human health research in ...
Tibbo, Markos
,
Schelling, E.
,
Grace, Delia
,
Bishop, Richard P.
,
Taracha, E.A.
,
Kemp, Stephen J.
,
Ameni, G.
,
Dawo, F.
,
Randolph, Thomas F.
,
[Cross-disciplinary and participatory livestock and human health research for successful control of zoonoses in the developing world]
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Cross-disciplinary and participatory livestock and human health research for successful control of zoonoses in the developing world
Iraqi, F.A.
,
Kemp, Stephen J.
,
Teale, A.J.
,
[Fine mapping of trypanosomiasis resistance QTLs in mice using advanced intercross lines]
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Fine mapping of trypanosomiasis resistance QTLs in mice using advanced intercross lines
We describe 10 polymorphic tetranucleotide microsatellite loci from the eastern Canary Island lacertid lizard, Gallotia atlantica. Loci were isolated from a partial genomic library that had been enriched for AAAG repeat sequence. All loci were highly polymorphic (eight alleles or more) with obser...
Bloor, P.
,
Laguna, I.H.B.
,
Kemp, Stephen J.
,
[Highly polymorphic tetranucleotide microsatellite loci for the eastern Canary Island lizard, Gallotia atlantica]
,
Highly polymorphic tetranucleotide microsatellite loci for the eastern Canary Island lizard, Gallotia atlantica
Under most circumstances, certain breeds of domestic ruminants show a remarkable resistance to the effects of African trypanosomiasis: they can tolerate the presence of parasites while apparently controlling levels of parasitaemia and, crucially, not showing the the severe anaemia and production ...
Kemp, Stephen J.
,
Nene, Vishvanath
,
[Biosciences research at the International Livestock Research Institute (ILRI)]
,
Biosciences research at the International Livestock Research Institute (ILRI)
BACKGROUND:
The advent of genotyping by Next Generation Sequencing has enabled rapid discovery of thousands of single nucleotide polymorphism (SNP) markers and high throughput genotyping of large populations at an affordable cost. Genotyping by sequencing (GBS), a reduced representation library s...
Onyango, M.G.
,
Aitken, N.C.
,
Jack, C.
,
Chuah, A.
,
Oguya, J.
,
Djikeng, Appolinaire
,
Kemp, Stephen J.
,
Bellis, G.A.
,
Nicholas, A.
,
Walker, P.J.
,
Duchemin, J.B.
,
[Genotyping of whole genome amplified reduced representation libraries reveals a cryptic population of Culicoides brevitarsis in the Northern Territory, Australia]
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Genotyping of whole genome amplified reduced representation libraries reveals a cryptic population of Culicoides brevitarsis in the Northern Territory, Australia
Hanotte, Olivier H.
,
Korol, A.
,
Agaba, Morris
,
Nilsson, P.
,
Gelhaus, P.
,
Horstmann, R.
,
Sugimoto, Y.
,
Kemp, Stephen J.
,
Soller, J.
,
Teale, A.J.
,
[Mapping of QTL controlling resistance to trypanosomosis in N'Dama X Boran cattle]
,
Mapping of QTL controlling resistance to trypanosomosis in N'Dama X Boran cattle