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Untiveros, M.Fuentes, S.Kreuze, J.F.[Molecular variability of sweet potato feathery mottle virus and other potyviruses infecting sweet potato in Peru.]Molecular variability of sweet potato feathery mottle virus and other potyviruses infecting sweet potato in Peru.

Sweet potato (Ipomoea batatas) is an important subsistence and famine reserve crop grown in developing countries where Sweet potato chlorotic stunt virus (SPCSV; Closteroviridae), a single-stranded RNA (ssRNA) crinivirus, synergizes unrelated viruses in co-infected sweet potato plants. The most s...


Cuéllar, Wilmer J.Kreuze, J.F.Rajamaki, M.L.Cruzado, K.R.Untiveros, M.Valkonen, J.P.T.[Elimination of antiviral defense by viral RNase III.]Elimination of antiviral defense by viral RNase III.

The largest extant RNA genomes are found in two diverse families of positive-strand RNA viruses, the animal Coronaviridae and the plant Closteroviridae. Comparative analysis of the viruses from the latter family reveals three levels of gene conservation. The most conserved gene module defines RNA...


Dolja, V.V.Kreuze, J.F.Valkonen, J.P.T.[Comparative and functional genomics of closteroviruses.]Comparative and functional genomics of closteroviruses.

We report the first identification of novel viruses, and sequence of an entire viral genome, by a single step of high-throughput parallel sequencing of small RNAs from diseased, as well as symptomless plants. Contigs were assembled from sequenced total siRNA from plants using small sequence assem...


Kreuze, J.F.Pérez, A.Untiveros, M.Quispe, D.Fuentes, S.Barker, I.Simon, R.[Complete viral genome sequence and discovery of novel viruses by deep sequencing of small RNAs: A generic method for diagnosis, discovery and sequencing of viruses.]Complete viral genome sequence and discovery of novel viruses by deep sequencing of small RNAs: A generic method for diagnosis, discovery and sequencing of viruses.

The plant virus family Flexiviridae includes the definitive genera Potexvirus, Mandarivirus, Allexivirus, Carlavirus, Foveavirus, Capillovirus, Vitivirus, Trichovirus, the putative genus Citrivirus, and some unassigned species. Its establishment was based on similarities in virion morphology, com...


Martelli, G.P.Adams, M.J.Kreuze, J.F.Dolja, V.V.[Family Flexiviridae: A case study in virion and genome plasticity.]Family Flexiviridae: A case study in virion and genome plasticity.

The complete nucleotide sequence of the isolate C1 of Sweet potato feathery mottle virus (SPFMV) strain C and the 5' region of several other strains were determined and analyzed together with the sequences of isolates representing the EA, RC and O strains. This provided molecular evidence for the...


Untiveros, M.Quispe, D.Kreuze, J.F.[Analysis of complete genomic sequences of isolates of the sweet potato feathery mottle virus strains C and EA: molecular evidence for two distinct potyvirus species and two P1 protein domains.]Analysis of complete genomic sequences of isolates of the sweet potato feathery mottle virus strains C and EA: molecular evidence for two distinct potyvirus species and two P1 protein domains.

One of the future challenges to produce food in a Mars environment will be the optimization of resources through the potential use of the Martian substratum for growing crops as a part of bioregenerative food systems. In vitro plantlets from 65 potato genotypes were rooted in peat-pellets substra...


Ramírez, D.Kreuze, J.F.Amoros, W.Valdivia Silva, J.E.Ranck, J.García, S.Salas, E.Yactayo, W.[Extreme salinity as a challenge to grow potatoes under Mars-like soil conditions: targeting promising genotypes.]Extreme salinity as a challenge to grow potatoes under Mars-like soil conditions: targeting promising genotypes.

Considered responsible for one million deaths in Ireland and widespread famine in the European continent during the 1840s, late blight, caused by Phytophthora infestans, remains the most devastating disease of potato (Solanum tuberosum L.) with about 15%–30% annual yield loss in sub‐Saharan Afric...


Ghislain, M.Byarugaba, A.A.Magembe, E.Njoroge, A.W.Rivera, C.Roman, M.L.Tovar, J.C.Gamboa, S.Forbes, G.Kreuze, J.F.Barekye, A.Kiggundu, A.[Stacking three late blight resistance genes from wild species directly into African highland potato varieties confers complete field resistance to local blight races]Stacking three late blight resistance genes from wild species directly into African highland potato varieties confers complete field resistance to local blight races

Accurate detection of viruses in plants and animals is critical for agriculture production and human health. Deep sequencing and assembly of virus-derived small interfering RNAs has proven to be a highly efficient approach for virus discovery. Here we present VirusDetect, a bioinformatics pipelin...


Yi ZhengShan GaoPadmanabhan, C.Li, R.Galvez, M.Gutiérrez, D.Fuentes, S.Kai-Shu LingKreuze, J.F.Zhangjun Fei[VirusDetect: an automated pipeline for efficient virus discovery using deep sequencing of small RNAs.]VirusDetect: an automated pipeline for efficient virus discovery using deep sequencing of small RNAs.

Virus‐related degeneration constrains production of quality sweet potato seed, especially under open field conditions. Once in the open, virus‐indexed seed is prone to virus infection leading to decline in performance. Insect‐proof net tunnels have been proven to reduce virus infection under rese...


Ogero, K.O.Kreuze, J.F.Mcewan, M.A.Luambano, N.D.Bachwenkizi, H.Garrett, K.A.Andersen, K.F.Thomas-Sharma, SaraVlugt, R.A.A.[Efficiency of insect‐proof net tunnels in reducing virus‐related seed degeneration in sweet potato.]Efficiency of insect‐proof net tunnels in reducing virus‐related seed degeneration in sweet potato.

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