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We report the first identification of novel viruses, and sequence of an entire viral genome, by a single step of high-throughput parallel sequencing of small RNAs from diseased, as well as symptomless plants. Contigs were assembled from sequenced total siRNA from plants using small sequence assem...


Kreuze, J.F.Pérez, A.Untiveros, M.Quispe, D.Fuentes, S.Barker, I.Simon, R.[Complete viral genome sequence and discovery of novel viruses by deep sequencing of small RNAs: A generic method for diagnosis, discovery and sequencing of viruses.]Complete viral genome sequence and discovery of novel viruses by deep sequencing of small RNAs: A generic method for diagnosis, discovery and sequencing of viruses.

Cuéllar, Wilmer J.Galvez, MarcoFuentes, S.Tugume, Joab K.Kreuze, J.F.[Synergistic interactions of begomoviruses with sweet potato chlorotic stunt virus (genus Crinivirus) in sweetpotato (Ipomoea batatas L.)]Synergistic interactions of begomoviruses with sweet potato chlorotic stunt virus (genus Crinivirus) in sweetpotato (Ipomoea batatas L.)
Abad, J.A.Li, R.Fuentes, S.Kreuze, J.F.Loschinkohl, C.Bandla, P.[Interception and identification by deep sequencing of a "caulimo-like" virus in a potato germplasm accession imported from South America.]Interception and identification by deep sequencing of a "caulimo-like" virus in a potato germplasm accession imported from South America.
Boonham, N.Kreuze, J.F.Winter, S.Vlugt, R. van der.Bergervoet, J.Tomlinson, J.Mumford, R.[Methods in virus diagnostics: From ELISA to next generation sequencing.]Methods in virus diagnostics: From ELISA to next generation sequencing.
Untiveros, M.Olspert, A.Firth, A.E.Artola, K.Kreuze, J.F.Valkonen, J.P.T.[1-38][A novel sweet potato potyvirus ORF is expressed via polymerase slippage and suppresses RNA silencing]A novel sweet potato potyvirus ORF is expressed via polymerase slippage and suppresses RNA silencing

Small interfering RNA (siRNA) duplexes are short (usually 21 to 24 bp) double-stranded RNAs (dsRNAs) with several overhanging nucleotides at both 5'- and 3'-ends. It has been found that siRNA duplexes bind the RNA-induced silencing complex (RISC) and cleave the sense strands with endonucleases. I...


Niu, X.Sun, Y.Chen, Z.Li, R.Padmanabhan, C.Ruan, J.Kreuze, J.F.Ling, K.S.Fei, Z.J.Gao, S.[Using small RNA-seq data to detect siRNA duplexes induced by plant viruses.]Using small RNA-seq data to detect siRNA duplexes induced by plant viruses.
Gibson, R.W.Kreuze, J.F.[Degeneration in sweetpotato due to viruses, virus-cleaned planting material and reversion: A review.]Degeneration in sweetpotato due to viruses, virus-cleaned planting material and reversion: A review.
Souza Richards, R.Adams, I.P.Kreuze, J.F.Souza, J. deCuéllar, Wilmer J.Dullemans, A.M.Vlugt, René A.A. van deGlover, R.Hany, U.Dickinson, M.Boonham, N.[The complete genome sequences of two isolates of potato black ringspot virus and their relationship to other isolates and nepoviruses.]The complete genome sequences of two isolates of potato black ringspot virus and their relationship to other isolates and nepoviruses.

El virus Y de la papa (PVY), virus representativo del genero Potyvirus, es uno de los virus de mayor incidencia en los cultivos de Solanaceas, tales como la papa, tabaco y pimiento. PVY existe como un complejo de variantes (strains) que son distinguidos principalmente por los sintomas desarrollad...


Flores, B.Silvestre, R.Muller, G.Villamil, A.Guzmán, M.Flores, M.Kreuze, J.F.[Analisis de la diversidad genetica de aislamientos de PVY infectando Solanum spp en los Andes mediante secuenciamiento de siRNA: Deteccion y caracterizacion de un nuevo linaje recombinante de PVY.]Analisis de la diversidad genetica de aislamientos de PVY infectando Solanum spp en los Andes mediante secuenciamiento de siRNA: Deteccion y caracterizacion de un nuevo linaje recombinante de PVY.

Andean potato mottle virus (APMoV, genero Comovirus) es un patogeno importante de varias plantas Solanaceas, que incluyen papa, tomate y tabaco, pero hasta la fecha solo esta disponible datos de secuencias parciales. En este estudio, se determino el genoma completo de APMoV utilizando secuenciami...


Souza, J. deMuller, G.Cuéllar, Wilmer J.Kreuze, J.F.[Secuencia genomica completa de un aislado peruano de Andean potato mottle virus (APMoV, genero Comovirus) utilizando secuenciamiento de siguiente generacion.]Secuencia genomica completa de un aislado peruano de Andean potato mottle virus (APMoV, genero Comovirus) utilizando secuenciamiento de siguiente generacion.

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