We report the first identification of novel viruses, and sequence of an entire viral genome, by a single step of high-throughput parallel sequencing of small RNAs from diseased, as well as symptomless plants. Contigs were assembled from sequenced total siRNA from plants using small sequence assem...
Kreuze, J.F.
,
Pérez, A.
,
Untiveros, M.
,
Quispe, D.
,
Fuentes, S.
,
Barker, I.
,
Simon, R.
,
[Complete viral genome sequence and discovery of novel viruses by deep sequencing of small RNAs: A generic method for diagnosis, discovery and sequencing of viruses.]
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Complete viral genome sequence and discovery of novel viruses by deep sequencing of small RNAs: A generic method for diagnosis, discovery and sequencing of viruses.
Cuéllar, Wilmer J.
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Galvez, Marco
,
Fuentes, S.
,
Tugume, Joab K.
,
Kreuze, J.F.
,
[Synergistic interactions of begomoviruses with sweet potato chlorotic stunt virus (genus Crinivirus) in sweetpotato (Ipomoea batatas L.)]
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Synergistic interactions of begomoviruses with sweet potato chlorotic stunt virus (genus Crinivirus) in sweetpotato (Ipomoea batatas L.)
Abad, J.A.
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Li, R.
,
Fuentes, S.
,
Kreuze, J.F.
,
Loschinkohl, C.
,
Bandla, P.
,
[Interception and identification by deep sequencing of a "caulimo-like" virus in a potato germplasm accession imported from South America.]
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Interception and identification by deep sequencing of a "caulimo-like" virus in a potato germplasm accession imported from South America.
Boonham, N.
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Kreuze, J.F.
,
Winter, S.
,
Vlugt, R. van der.
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Bergervoet, J.
,
Tomlinson, J.
,
Mumford, R.
,
[Methods in virus diagnostics: From ELISA to next generation sequencing.]
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Methods in virus diagnostics: From ELISA to next generation sequencing.
Small interfering RNA (siRNA) duplexes are short (usually 21 to 24 bp) double-stranded RNAs (dsRNAs) with several overhanging nucleotides at both 5'- and 3'-ends. It has been found that siRNA duplexes bind the RNA-induced silencing complex (RISC) and cleave the sense strands with endonucleases. I...
Niu, X.
,
Sun, Y.
,
Chen, Z.
,
Li, R.
,
Padmanabhan, C.
,
Ruan, J.
,
Kreuze, J.F.
,
Ling, K.S.
,
Fei, Z.J.
,
Gao, S.
,
[Using small RNA-seq data to detect siRNA duplexes induced by plant viruses.]
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Using small RNA-seq data to detect siRNA duplexes induced by plant viruses.
Gibson, R.W.
,
Kreuze, J.F.
,
[Degeneration in sweetpotato due to viruses, virus-cleaned planting material and reversion: A review.]
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Degeneration in sweetpotato due to viruses, virus-cleaned planting material and reversion: A review.
Souza Richards, R.
,
Adams, I.P.
,
Kreuze, J.F.
,
Souza, J. de
,
Cuéllar, Wilmer J.
,
Dullemans, A.M.
,
Vlugt, René A.A. van de
,
Glover, R.
,
Hany, U.
,
Dickinson, M.
,
Boonham, N.
,
[The complete genome sequences of two isolates of potato black ringspot virus and their relationship to other isolates and nepoviruses.]
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The complete genome sequences of two isolates of potato black ringspot virus and their relationship to other isolates and nepoviruses.
El virus Y de la papa (PVY), virus representativo del genero Potyvirus, es uno de los virus de mayor incidencia en los cultivos de Solanaceas, tales como la papa, tabaco y pimiento. PVY existe como un complejo de variantes (strains) que son distinguidos principalmente por los sintomas desarrollad...
Flores, B.
,
Silvestre, R.
,
Muller, G.
,
Villamil, A.
,
Guzmán, M.
,
Flores, M.
,
Kreuze, J.F.
,
[Analisis de la diversidad genetica de aislamientos de PVY infectando Solanum spp en los Andes mediante secuenciamiento de siRNA: Deteccion y caracterizacion de un nuevo linaje recombinante de PVY.]
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Analisis de la diversidad genetica de aislamientos de PVY infectando Solanum spp en los Andes mediante secuenciamiento de siRNA: Deteccion y caracterizacion de un nuevo linaje recombinante de PVY.
Andean potato mottle virus (APMoV, genero Comovirus) es un patogeno importante de varias plantas Solanaceas, que incluyen papa, tomate y tabaco, pero hasta la fecha solo esta disponible datos de secuencias parciales. En este estudio, se determino el genoma completo de APMoV utilizando secuenciami...
Souza, J. de
,
Muller, G.
,
Cuéllar, Wilmer J.
,
Kreuze, J.F.
,
[Secuencia genomica completa de un aislado peruano de Andean potato mottle virus (APMoV, genero Comovirus) utilizando secuenciamiento de siguiente generacion.]
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Secuencia genomica completa de un aislado peruano de Andean potato mottle virus (APMoV, genero Comovirus) utilizando secuenciamiento de siguiente generacion.