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Cuéllar, Wilmer J.Cruzado, R.Fuentes, S.Untiveros, M.Soto, M.Kreuze, J.F.[Sequence characterization of a Peruvian isolate of sweet potato chlorotic stunt virus: Further variability and a model for p22 acquisition.]Sequence characterization of a Peruvian isolate of sweet potato chlorotic stunt virus: Further variability and a model for p22 acquisition.

An inverted repeat construct corresponding to a segment of the potato leaf roll virus coat protein gene was created under control of a constitutive promoter and transferred into a transformation vector with a heat inducible Cre-loxP system to excise the nptII antibiotic resistance marker gene. Fi...


Orbegozo, JeanetteSolorzano, DennisCuéllar, Wilmer J.Bartolini, Maria LupeGhislain, M.Kreuze, J.F.[Marker-free PLRV resistant potato mediated by Cre-loxP excision and RNAi]Marker-free PLRV resistant potato mediated by Cre-loxP excision and RNAi

Reverse-transcribing viruses, which synthesize a copy of genomic DNA from an RNA template, are widespread in animals, plants, algae, and fungi. This broad distribution suggests the ancient origin(s) of these viruses, possibly concomitant with the emergence of eukaryotes. Reverse-transcribing viru...


Hull, R.Johnson, W.Kreuze, J.F.Lindemann, D.Llorens, C.Lockhart, B.E.L.Mayer, J.Muller, E.Olszewski, N.E.Pappu, H.R.Pooggin, M.M.Richert-Poggeler, K.R.Sabanadzovic, S.Sanfacon, H.Schoelz, J.E.Seal, S.Stavolone, L.Stoye, J.P.Teycheney, P.Y.Tristem, M.Koonin, E.V.Kuhn, J.H.Krupovic, M.Blomberg, J.Coffin, J.M.Dasgupta, I.Fan, H.Geering, A.D.Gifford, R.Harrach, B.[Ortervirales: New virus order unifying five families of reverse-transcribing viruses]Ortervirales: New virus order unifying five families of reverse-transcribing viruses

Sweetpotato chlorotic stunt virus (SPCSV; genus Crinivirus, family Closteroviridae) is one of the most important pathogens of sweetpotato (Ipomoea batatas L.). It can reduce yields by 50% by itself and cause various synergistic disease complexes when co-infecting with other viruses, including swe...


Kreuze, J.F.Klein, I.S.Ghislain, M.Valkonen, J.P.T.Untiveros, M.Cuéllar, Wilmer J.Lajo, G.Cipriani, P.[RNA silencing-mediated resistance to a crinivirus (Closteroviridae) in cultivated sweetpotato (Ipomoea batatas L.) and development of sweetpotato virus disease following co-infection with a potyvirus.]RNA silencing-mediated resistance to a crinivirus (Closteroviridae) in cultivated sweetpotato (Ipomoea batatas L.) and development of sweetpotato virus disease following co-infection with a potyvirus.

Double-stranded RNA (dsRNA)-specific endonucleases belonging to RNase III classes 3 and 2 process dsRNA precursors to small interfering RNA (siRNA) or microRNA, respectively, thereby initiating and amplifying RNA silencing-based antiviral defense and gene regulation in eukaryotic cells. However, ...


Kreuze, J.F.Savenkov, E.I.Cuéllar, Wilmer J.Xiangdong LiValkonen, J.P.T.[Viral class 1 RNase III involved in suppression of RNA silencing.]Viral class 1 RNase III involved in suppression of RNA silencing.

Sweet potato (Ipomoea batatas) is an important subsistence and famine reserve crop grown in developing countries where Sweet potato chlorotic stunt virus (SPCSV; Closteroviridae), a single-stranded RNA (ssRNA) crinivirus, synergizes unrelated viruses in co-infected sweet potato plants. The most s...


Cuéllar, Wilmer J.Kreuze, J.F.Rajamaki, M.L.Cruzado, K.R.Untiveros, M.Valkonen, J.P.T.[Elimination of antiviral defense by viral RNase III.]Elimination of antiviral defense by viral RNase III.

The plant virus family Flexiviridae includes the definitive genera Potexvirus, Mandarivirus, Allexivirus, Carlavirus, Foveavirus, Capillovirus, Vitivirus, Trichovirus, the putative genus Citrivirus, and some unassigned species. Its establishment was based on similarities in virion morphology, com...


Martelli, G.P.Adams, M.J.Kreuze, J.F.Dolja, V.V.[Family Flexiviridae: A case study in virion and genome plasticity.]Family Flexiviridae: A case study in virion and genome plasticity.

The complete nucleotide sequence of the isolate C1 of Sweet potato feathery mottle virus (SPFMV) strain C and the 5' region of several other strains were determined and analyzed together with the sequences of isolates representing the EA, RC and O strains. This provided molecular evidence for the...


Untiveros, M.Quispe, D.Kreuze, J.F.[Analysis of complete genomic sequences of isolates of the sweet potato feathery mottle virus strains C and EA: molecular evidence for two distinct potyvirus species and two P1 protein domains.]Analysis of complete genomic sequences of isolates of the sweet potato feathery mottle virus strains C and EA: molecular evidence for two distinct potyvirus species and two P1 protein domains.

Cuéllar, Wilmer J.Galvez, MarcoFuentes, S.Tugume, Joab K.Kreuze, J.F.[Synergistic interactions of begomoviruses with sweet potato chlorotic stunt virus (genus Crinivirus) in sweetpotato (Ipomoea batatas L.)]Synergistic interactions of begomoviruses with sweet potato chlorotic stunt virus (genus Crinivirus) in sweetpotato (Ipomoea batatas L.)
Souza, J. deFuentes, S.Savenkov, E.I.Cuéllar, Wilmer J.Kreuze, J.F.[The complete nucleotide sequence of sweet potato C6 virus : a carlavirus lacking a cysteine-rich protein]The complete nucleotide sequence of sweet potato C6 virus : a carlavirus lacking a cysteine-rich protein

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