In July 2011, banana and plantain that displayed stunting and leaf symptoms typical of banana bunchy top disease were observed to be widespread in Dangbo Commune, Ouémé Department, Benin. To identify the cause of the disease, a roving survey was conducted in December 2011 in nine locations in Avr...
Lokossou, B.
,
Gnanvossou, D.
,
Ayodeji, O.
,
Akplogan, F.
,
Safiore, A.
,
Migan, D.
,
Pefoura, A.
,
Hanna, R.
,
Kumar, P.L.
,
[Occurrence of Banana bunchy top virus in banana and plantain (Musa sp.) in Benin]
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Occurrence of Banana bunchy top virus in banana and plantain (Musa sp.) in Benin
The fall armyworm Spodoptera frugiperda is a prime noctuid pest of maize on the American continents where it has remained confined despite occasional interceptions by European quarantine services in recent years. The pest has currently become a new invasive species in West and Central Africa wher...
Goergen, Georg E.
,
Kumar, P.L.
,
Sankung, S.B.
,
Togola, A.
,
Tamò, M.
,
[First report of outbreaks of the fall armyworm Spodoptera Frugiperda (J E Smith) (Lepidoptera, Noctuidae), a new alien invasive pest in West and Central Africa]
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First report of outbreaks of the fall armyworm Spodoptera Frugiperda (J E Smith) (Lepidoptera, Noctuidae), a new alien invasive pest in West and Central Africa
Cassava mosaic disease (CMD) caused by African cassava mosaic virus (ACMV) and East African cassava mosaic Cameroon virus (EACMCV) is the major constraint to cassava production in Nigeria. Sequences of the DNA-A component of ACMV and EACMCV isolates from leguminous plant species (Senna occidental...
During routine surveys conducted from February to July 2015 in the northern guinea savannah
region of Nigeria, sugarcane and itch grass (Rottboellia cochinchinensis) plants showing virus-like
mosaic symptoms were encountered in farmers’ sugarcane fields in Kaduna State. Symptomatic
leaf tissue sa...
Polymerase chain reaction (PCR)-based applications in plant molecular biology and molecular diagnostics for plant pathogens require good quality DNA for reliable and reproducible results. Leaf tissue is often the choice for DNA extraction, but the use of other sources such as tubers, stems, or se...
Sharma, K.
,
Bhattacharjee, R.
,
Sartie, A.M.
,
Kumar, P.L.
,
[An improved method of DNA extraction from plants for pathogen detection and genotyping by polymerase chain reaction]
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An improved method of DNA extraction from plants for pathogen detection and genotyping by polymerase chain reaction
Uniplex and multiplex reverse transcription-polymerase chain reaction (RT-PCR) protocols were developed for the detection of cassava brown streak viruses (CBSVs) in single and mixed infections with cassava mosaic begomoviruses (CMBs) in a tropical crop plant, cassava (Manihot esculenta). CMBs con...
Abarshi, M.M.
,
Mohammed, I.
,
Jeremiah, S.C.
,
Legg, J.P.
,
Kumar, P.L.
,
Hillocks, R.J.
,
Maruthi, M.N.
,
[Multiplex RTPCR assays for the simultaneous detection of both RNA and DNA viruses infecting cassava and the common occurrence of mixed infections by two cassava brown streak viruses in East Africa]
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Multiplex RTPCR assays for the simultaneous detection of both RNA and DNA viruses infecting cassava and the common occurrence of mixed infections by two cassava brown streak viruses in East Africa
Aims: To develop a multiplex TaqMan-based real-time PCR assay (qPCR) for the simultaneous detection and quantification of both RNA and DNA viruses affecting cassava (Manihot esculenta) in eastern Africa.
Methods and Results: The diagnostic assay was developed for two RNA viruses; Cassava brown st...
Otti, G.
,
Bouvaine, S.
,
Kimata, B.
,
Mkamillo, G.
,
Kumar, P.L.
,
Tomlins, Keith I.
,
Maruthi, M.N.
,
[High-throughput multiplex real-time PCR assay for the simultaneous quantification of DNA and RNA viruses infecting cassava plants]
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High-throughput multiplex real-time PCR assay for the simultaneous quantification of DNA and RNA viruses infecting cassava plants
Banana bunchy top disease (BBTD) is a serious threat to banana and plantain (Musa spp.) production. BBTD is caused by the Banana bunchy top virus (BBTV, genus Babuvirus) which is spread through infected plant propagules and banana aphid, Pentalonia nigronervosa. A high level of resistance to BBTD...
Ngatat, S.
,
Hanna, R.
,
Kumar, P.L.
,
Gray, S.M.
,
Cilia, M.
,
Ghogomu, R.T.
,
Fontem, D.A.
,
[Relative susceptibility of Musa genotypes to banana bunchy top disease in Cameroon and implication for disease management]
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Relative susceptibility of Musa genotypes to banana bunchy top disease in Cameroon and implication for disease management
The root-knot nematodes (RKN), Meloidogyne spp., represent an important threat to yam (Dioscorea spp.) production in West Africa. With the aim to establish the diversity of RKN species affecting yam tubers, for control and resistance screening purposes, surveys were conducted in the main yam prod...
Kolombia, Y.A.
,
Karssen, G.
,
Viaene, N.
,
Kumar, P.L.
,
Sutter, N. de
,
Joos, L.
,
Coyne, D.L.
,
Bert, W.
,
[Diversity of root-knot nematodes associated with tubers of yam (Dioscorea spp.) established using isozyme analysis and mitochondrial DNA-based identification]
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Diversity of root-knot nematodes associated with tubers of yam (Dioscorea spp.) established using isozyme analysis and mitochondrial DNA-based identification
Two new ‘legumoviruses’ (genus Begomovirus; family Geminiviridae) naturally infecting soybean (Glycine max L. Merr.) in Nigeria were molecularly characterized. Based on characteristic symptoms in soybean, the two viruses are provisionally designated as Soybean mild mottle virus (SbMMV) and Soybea...