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Cassava mosaic disease (CMD) caused by African cassava mosaic virus (ACMV) and East African cassava mosaic Cameroon virus (EACMCV) is the major constraint to cassava production in Nigeria. Sequences of the DNA-A component of ACMV and EACMCV isolates from leguminous plant species (Senna occidental...


Alabi, O.J.Ogbe, F.O.Bandyopadhyay, RanajitKumar, P.L.Dixon, Alfred G.O.Hughes, Jacqueline d'ArrosNaidu, R.A.[Alternate hosts of African cassava mosaic virus and East African cassava mosaic Cameroon virus in Nigeria]Alternate hosts of African cassava mosaic virus and East African cassava mosaic Cameroon virus in Nigeria

During routine surveys conducted from February to July 2015 in the northern guinea savannah region of Nigeria, sugarcane and itch grass (Rottboellia cochinchinensis) plants showing virus-like mosaic symptoms were encountered in farmers’ sugarcane fields in Kaduna State. Symptomatic leaf tissue sa...


Yahaya, A.Al Rwahnih, M.Dangora, D.B.Gregg, L.Alegbejo, M.D.Kumar, P.L.Alabi, O.J.[First report of maize yellow mosaic virus infecting sugarcane (Saccharum spp.) and itch grass (Rottboellia cochinchinensis) in Nigeria]First report of maize yellow mosaic virus infecting sugarcane (Saccharum spp.) and itch grass (Rottboellia cochinchinensis) in Nigeria

African cassava mosaic virus (ACMV; genus Begomovirus, family Geminiviridae) is one of six viruses documented in cassava (Manihot esculenta Crantz.) plants showing cassava mosaic disease in sub-Saharan Africa (SSA). In addition to cassava, the natural host range of ACMV includes a few wild Maniho...


Mgbechi-Ezeri, J.U.Alabi, O.J.Naidu, R.A.Kumar, P.L.[First report of the occurrence of African cassava mosaic virus in a mosaic disease of soybean in Nigeria]First report of the occurrence of African cassava mosaic virus in a mosaic disease of soybean in Nigeria

Helicoverpa armigera nucleopolyhedrovirus (HaNPV, Family: Baculoviridae; Genus: Nucleopolyhedrovirus) is a natural pathogen of Helicoverpa armigera (Insecta: Arthropoda) larvae and has proven to be a good candidate for the biocontrol of this pest on legume crops. In this study various immunochemi...


Kumar, C.S.Sireesha, K.Rao, G.V.Reddy, A.S.Rambabu, C.Kumar, P.L.[Development and evaluation of immunochemical tools for diagnosis and quality control of Helicoverpa armigera nucleopolyhedrovirus (HaNPV)]Development and evaluation of immunochemical tools for diagnosis and quality control of Helicoverpa armigera nucleopolyhedrovirus (HaNPV)

Cassava is the most important root and tuber crop in Sierra Leone. Its low yield can be attributed to several production-limiting factors including cassava mosaic disease (CMD). This study examined in a much wider scope the diversity, prevalence, distribution, incidence and level of severity of t...


Samura, A.E.Massaquoi, F.B.Mansaray, A.Kumar, P.L.Koroma, J.P.C.Fomba, S.N.Dixon, A.[Status and diversity of the cassava Mosaic disease causal agents in Sierra Leone]Status and diversity of the cassava Mosaic disease causal agents in Sierra Leone

Polymerase chain reaction (PCR)-based applications in plant molecular biology and molecular diagnostics for plant pathogens require good quality DNA for reliable and reproducible results. Leaf tissue is often the choice for DNA extraction, but the use of other sources such as tubers, stems, or se...


Sharma, K.Bhattacharjee, R.Sartie, A.M.Kumar, P.L.[An improved method of DNA extraction from plants for pathogen detection and genotyping by polymerase chain reaction]An improved method of DNA extraction from plants for pathogen detection and genotyping by polymerase chain reaction

Uniplex and multiplex reverse transcription-polymerase chain reaction (RT-PCR) protocols were developed for the detection of cassava brown streak viruses (CBSVs) in single and mixed infections with cassava mosaic begomoviruses (CMBs) in a tropical crop plant, cassava (Manihot esculenta). CMBs con...


Abarshi, M.M.Mohammed, I.Jeremiah, S.C.Legg, J.P.Kumar, P.L.Hillocks, R.J.Maruthi, M.N.[Multiplex RTPCR assays for the simultaneous detection of both RNA and DNA viruses infecting cassava and the common occurrence of mixed infections by two cassava brown streak viruses in East Africa]Multiplex RTPCR assays for the simultaneous detection of both RNA and DNA viruses infecting cassava and the common occurrence of mixed infections by two cassava brown streak viruses in East Africa

Many Nigerian farmers depend for their seed on seed-producing farmers, the so-called informal Seed System (SS), but seed quality of the SS is unknown. Farmers planting low quality seed risk poor field emergence and low plant vigour as a result of low physiological quality or infection with seed-b...


Biemond, P.C.Oguntade, O.Stomph, T.Kumar, P.L.Termorshuizen, A.J.Struik, Paul C.[Health of farmersaved maize seed in northeast Nigeria]Health of farmersaved maize seed in northeast Nigeria

Aims: To develop a multiplex TaqMan-based real-time PCR assay (qPCR) for the simultaneous detection and quantification of both RNA and DNA viruses affecting cassava (Manihot esculenta) in eastern Africa. Methods and Results: The diagnostic assay was developed for two RNA viruses; Cassava brown st...


Otti, G.Bouvaine, S.Kimata, B.Mkamillo, G.Kumar, P.L.Tomlins, Keith I.Maruthi, M.N.[High-throughput multiplex real-time PCR assay for the simultaneous quantification of DNA and RNA viruses infecting cassava plants]High-throughput multiplex real-time PCR assay for the simultaneous quantification of DNA and RNA viruses infecting cassava plants

Cassava mosaic disease (CMD), caused by different species of cassava mosaic geminiviruses (CMGs), is themost important disease of cassava in Africa and the Indian sub-continent. The cultivated cassava speciesis protected from CMD by polygenic resistance introgressed from the wild species Manihot ...


Rabbi, Ismail Y.Hamblin, M.Kumar, P.L.Gedil, M.Ikpan, A.Jannink, Jean-LucKulakow, P.A.[High-resolution mapping of resistance to cassava mosaic geminiviruses in cassava using genotypingbysequencing and its implications for breeding]High-resolution mapping of resistance to cassava mosaic geminiviruses in cassava using genotypingbysequencing and its implications for breeding

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