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Journal Article 2016-05

High-throughput multiplex real-time PCR assay for the simultaneous quantification of DNA and RNA viruses infecting cassava plants

Autor: Otti, G. Bouvaine, S. Kimata, B. Mkamillo, G. Kumar, P.L. Tomlins, Keith I. Maruthi, M.N.

Aims: To develop a multiplex TaqMan-based real-time PCR assay (qPCR) for the simultaneous detection and quantification of both RNA and DNA viruses affecting cassava (Manihot esculenta) in eastern Africa. Methods and Results: The diagnostic assay was developed for two RNA viruses; Cassava brown st...

Enlace original: https://cgspace.cgiar.org/handle/10568/76987

Investigación en Agricultura

CASSAVA

DNA

PLANT DISEASES

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Otti, G. , Bouvaine, S. , Kimata, B. , Mkamillo, G. , Kumar, P.L. , Tomlins, Keith I. , Maruthi, M.N. , [High-throughput multiplex real-time PCR assay for the simultaneous quantification of DNA and RNA viruses infecting cassava plants] , High-throughput multiplex real-time PCR assay for the simultaneous quantification of DNA and RNA viruses infecting cassava plants

Journal Article 2018-06-22

Seed transmissibility of Cucumber mosaic virus in Capsicum species

Autor: Arogundade, O. Balogun, O.S. Kumar, P.L.

Cucumber mosaic virus (CMV) occurs worldwide and is a harmful pathogen for pepper (Capsicum spp.). Transmission of the virus is normally by insect. The possibility of transmission by seed is less clear. Accessions collected from eight states in Nigeria were evaluated to determine seed transmissib...

Enlace original: https://cgspace.cgiar.org/handle/10568/96161

Investigación en Agricultura

CAPISCUM ANNUM

CUCUMBER MOSAIC VIRUS

SEEDLINGS

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Arogundade, O. , Balogun, O.S. , Kumar, P.L. , [Seed transmissibility of Cucumber mosaic virus in Capsicum species] , Seed transmissibility of Cucumber mosaic virus in Capsicum species

Journal Article 2014

The prevalence of badnaviruses in West African yams (Dioscorea cayenensisrotundata) and evidence of endogenous pararetrovirus sequences in their genomes

Autor: Seal, S. Turaki, A. Muller, E. Kumar, P.L. Kenyon, L. Filloux, D. Galzi, S. López Montes, A. Iskra Caruana, M.L.

Yam (Dioscorea spp.) is an important vegetatively-propagated staple crop in West Africa. Viruses arepervasive in yam worldwide, decreasing growth and yield, as well as hindering the international move-ment of germplasm. Badnaviruses have been reported to be the most prevalent in yam, and genomeso...

Enlace original: https://cgspace.cgiar.org/handle/10568/75930

Investigación en Agricultura

ELISA

PCR

DIOSCOREA

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Seal, S. , Turaki, A. , Muller, E. , Kumar, P.L. , Kenyon, L. , Filloux, D. , Galzi, S. , López Montes, A. , Iskra Caruana, M.L. , [The prevalence of badnaviruses in West African yams (Dioscorea cayenensisrotundata) and evidence of endogenous pararetrovirus sequences in their genomes] , The prevalence of badnaviruses in West African yams (Dioscorea cayenensisrotundata) and evidence of endogenous pararetrovirus sequences in their genomes

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High-throughput multiplex real-time PCR assay for the simultaneous quantification of DNA and RNA viruses infecting cassava plants

Seed transmissibility of Cucumber mosaic virus in Capsicum species

The prevalence of badnaviruses in West African yams (Dioscorea cayenensisrotundata) and evidence of endogenous pararetrovirus sequences in their genomes