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Polymerase chain reaction (PCR)-based applications in plant molecular biology and molecular diagnostics for plant pathogens require good quality DNA for reliable and reproducible results. Leaf tissue is often the choice for DNA extraction, but the use of other sources such as tubers, stems, or se...


Sharma, K.Bhattacharjee, R.Sartie, A.M.Kumar, P.L.[An improved method of DNA extraction from plants for pathogen detection and genotyping by polymerase chain reaction]An improved method of DNA extraction from plants for pathogen detection and genotyping by polymerase chain reaction

The efficacy of four biological control agents (BCAs): Trichoderma asperellum strain NGT158, T. longibrachiatum strain NGT167, Bacillus subtilis and Pseudomonas fluorescens for the management of post-harvest tuber rot among four yam species, Dioscorea rotundata, D. cayenensis, D. alata, and D. du...


Dania, V.O.Fadina, O.O.Ayodele, M.Kumar, P.L.[Evaluation of isolates of Trichoderma, pseudomonas and bacillus species as treatment for the control of post-harvest fungal rot disease of yam (Dioscorea spp.)]Evaluation of isolates of Trichoderma, pseudomonas and bacillus species as treatment for the control of post-harvest fungal rot disease of yam (Dioscorea spp.)

Biological control is a significant component of integrated pest management programs around the world. However, uncertainty about the risk of unanticipated effects on non-target organisms is one of the major concerns surrounding the release of biological control agents. This Brief has been prepar...


Hoeschle-Zeledon, IrmgardNeuenschwander, P.Kumar, P.L.[Regulatory challenges for biological control]Regulatory challenges for biological control

Diseases caused by viral, fungal and bacterial pathogens pose great threat to food security in Africa. Use of disease resistant cultivars is among the most preferred tactic for disease control by smallholder farmers in Africa, who cannot afford cost intensive chemical inputs or labor intensive cr...


Tripathi, L.Kumar, P.L.Patil, B.L.Fatokun, C.A.[Pathogen resistant genetically engineered crops in Africa]Pathogen resistant genetically engineered crops in Africa

Field surveys were conducted in 2012 and 2013 to evaluate the distribution and virulence of fungal isolates associated with tuber rot disease among yam (Dioscorea spp.) producing agroecological zones (AEZs) of Nigeria. A total of 70 and 66 farmers’ fields were surveyed in 2012 and 2013, respectiv...


Dania, V.O.Fadina, O.O.Ayodele, M.Kumar, P.L.[Distribution and virulence of fungal species isolated from yam (Dioscorea spp.) tubers in three agroecological zones of Nigeria]Distribution and virulence of fungal species isolated from yam (Dioscorea spp.) tubers in three agroecological zones of Nigeria

Tuber rot disease is a major constraint to white yam (Dioscorea rotundata) production, accounting for 50-60% of annual yield losses in Nigeria. The main method of control using synthetic fungicides is being discouraged due to human and environmental health hazards. The potential of Oryza sativa h...


Dania, V.O.Fadina, O.Ayodele, M.Kumar, P.L.[Efficacy of Oryza sativa husk and Quercus phillyraeoides extracts for the in vitro and in vivo control of fungal rot disease of white yam (Dioscorea rotundata Poir)]Efficacy of Oryza sativa husk and Quercus phillyraeoides extracts for the in vitro and in vivo control of fungal rot disease of white yam (Dioscorea rotundata Poir)

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