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Cassava brown streak disease (CBSD) is the most important virus disease of cassava and a major food security threat in Africa. Yearly economic losses of up to $100 million USD have been attributed to CBSD. The lack of information on plant-virus interactions has restricted progress in breeding for...


Shirima, R.R.Maeda, D.GKanju, E.Ceasar, G.Tibazarwa, F.I.Legg, J.P.[Absolute quantification of cassava brown streak virus mRNA by real-time qPCR]Absolute quantification of cassava brown streak virus mRNA by real-time qPCR

Bemisia tabaci threatens production of cassava in Africa through vectoring viruses that cause cassava mosaic disease (CMD) and cassava brown streak disease (CBSD). B. tabaci sampled from cassava in eight countries in Africa were genotyped using NextRAD sequencing, and their phylogeny and populati...


Wosula, Everlyne N.Chen, W.Fei, Z.Legg, J.P.[Unravelling the genetic diversity among cassava Bemisia tabaci whiteflies using NextRAD Sequencing]Unravelling the genetic diversity among cassava Bemisia tabaci whiteflies using NextRAD Sequencing

Cassava mosaic disease (CMD) is a major constraint to sustainable production of cassava (Manihot esculenta Crantz) in Zambia and other sub-Saharan African countries. During a survey conducted between April and May 2014 in six (Western, Northwestern, Northern, Luapula, Lusaka, and Eastern) provinc...


Mulenga, R.Miano, D.Chikoti, Patrick ChizaNdunguru, J.Legg, J.P.Alabi, O.J.[First report of East African cassava mosaic Malawi virus in plants affected by cassava mosaic disease in Zambia]First report of East African cassava mosaic Malawi virus in plants affected by cassava mosaic disease in Zambia

Uniplex and multiplex reverse transcription-polymerase chain reaction (RT-PCR) protocols were developed for the detection of cassava brown streak viruses (CBSVs) in single and mixed infections with cassava mosaic begomoviruses (CMBs) in a tropical crop plant, cassava (Manihot esculenta). CMBs con...


Abarshi, M.M.Mohammed, I.Jeremiah, S.C.Legg, J.P.Kumar, P.L.Hillocks, R.J.Maruthi, M.N.[Multiplex RTPCR assays for the simultaneous detection of both RNA and DNA viruses infecting cassava and the common occurrence of mixed infections by two cassava brown streak viruses in East Africa]Multiplex RTPCR assays for the simultaneous detection of both RNA and DNA viruses infecting cassava and the common occurrence of mixed infections by two cassava brown streak viruses in East Africa
Maruthi, M.N.Whitfield, E.C.Kumar, P.L.Legg, J.P.[Virus-indexing, chemo-and thermo-therapies, and micro propagation for generating virus-free cassava plants.]Virus-indexing, chemo-and thermo-therapies, and micro propagation for generating virus-free cassava plants.

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