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Interleukin-3(IL-3) is one of the cytokines that act during the early and late stages of blood cell formation. To enable the study of the role of IL-3 in bovine haemopoietic stem cell differentiation, the polymerase chain reaction was used to amplify an IL-3 cDNA from first-strand cDNAs prepared ...


Mwangi, S.M.Logan-Henfrey, L.L.McInnes, C.Mertens, B.[Cloning of the bovine interleukin-3-encoding cDNA. (Colony-stimulating factor: Cytokine: PCR: Neuclotide sequence: RACE)]Cloning of the bovine interleukin-3-encoding cDNA. (Colony-stimulating factor: Cytokine: PCR: Neuclotide sequence: RACE)

Five adult Boran cattle (Bos indicus), infected with a clone of Trypanosoma congolense IL13-E3 three years earlier and treated, were re-challenged with the same clone. Changes in the peripheral blood were monitored twice weekly, and events in the bone marrow (BM) were assessed by weekly biopsies ...


Anosa, V.O.Logan-Henfrey, L.L.Wells, C.W.[The haematology of Trypanosoma congolense infection in cattle. I. Sequential cytomorphological changes in the blood and bone marrow of Boran cattle]The haematology of Trypanosoma congolense infection in cattle. I. Sequential cytomorphological changes in the blood and bone marrow of Boran cattle

Sequential biopsies of sternal bone marrow (BM) of three trypanosusceptible Boran and three trypanotolerant N'Dama cattle were examined by light and transmission electron microscopy before and up to 112 days postinfection (DPI) with Trypanosoma congolense. Before infection, the percentage of cell...


Anosa, V.O.Logan-Henfrey, L.L.Wells, C.W.[The role of the bone marrow in bovine trypanotolerance. II. Macrophage function in Trypanosoma congolense - infected cattle]The role of the bone marrow in bovine trypanotolerance. II. Macrophage function in Trypanosoma congolense - infected cattle

Macrophages (Mo) in smears and sections of sternal bone marrow (BM) derived by weekly sequential biopsies from five adult Boran cattle re-challenged with Trypanosoma congolense were studied by light and transmission electron microscopy (TEM). Cells of the mononuclear phagocyte system including mo...


Anosa, V.O.Logan-Henfrey, L.L.Wells, C.W.[The haematology of Trypanosoma congolense infection in cattle. II. Macrophage structure and function in the bone marrow of Boran cattle]The haematology of Trypanosoma congolense infection in cattle. II. Macrophage structure and function in the bone marrow of Boran cattle

This study compared the changes in the bone marrow (BM) of five trypanotolerant N'Dama cattle with those of four trypanosusceptible Boran cattle during trypanosome infection. In the early parasitaemic phase, from 12 to 21 days postinfection (DPI), tsetse transmitted primary Trypanosoma congolense...


Logan-Henfrey, L.L.Anosa, V.O.Wells, C.W.[The role of the bone marrow in bovine trypanotolerance. 1. Changes in blood and bone marrow in Trypanosoma congolense-infected cattle]The role of the bone marrow in bovine trypanotolerance. 1. Changes in blood and bone marrow in Trypanosoma congolense-infected cattle

The cDNA coding for the soluble form of bovine stem cell factor (boSCF-Ala-105) was cloned and recombinant protein was produced in bacteria as a histidine tagged-protein. The protein was purified from the inclusion bodies in one step by metal chelation chromatography under denaturing conditions. ...


Mertens, B.Muriuki, C.Muiya, P.Andrianarivo, A.Mwangi, S.Logan-Henfrey, L.L.[Bovine stem cell factor: Production of a biologically active protein and mRNA analysis in cattle infected with Trypanosoma congolense]Bovine stem cell factor: Production of a biologically active protein and mRNA analysis in cattle infected with Trypanosoma congolense
Sileghem, M.R.Flynn, J.N.Logan-Henfrey, L.L.Ellis, J.[Tumour necrosis factor production by monocytes from cattle infected with Trypanosoma (Duttonella) vivax and Trypanosoma (Nannomonas) congolense STI possible association with severity of anaemia associated with the disease]Tumour necrosis factor production by monocytes from cattle infected with Trypanosoma (Duttonella) vivax and Trypanosoma (Nannomonas) congolense STI possible association with severity of anaemia associated with the disease

Acute Trypanosoma congolense infection induced moderate, transient anemia in N'Dama cattle (trypanotolerant) and severe anemia in Boran cattle (trypanosusceptible). Erythropoietin receptor (EpoR) was cloned and sequenced from the two breeds of cattle. A single position mutation of Tyr in the Bora...


Suliman, H.B.Logan-Henfrey, L.L.Majiwa, P.A.O.Ole-MoiYoi, O.K.Feldman, B.F.[Analysis of erythropoietin and erythropoietin receptor genes expression in cattle during acute infection with Trypanosoma congolense]Analysis of erythropoietin and erythropoietin receptor genes expression in cattle during acute infection with Trypanosoma congolense

Trypanosomiasis is a serious constraint to livestock production in sub-Saharan Africa. Some breeds of cattle are genetically more resistant to the pathogenic effects of trypanosome infection. We measured B-cell activation and the quantity and isotype of antibody produced at the cellular level in ...


Taylor, K.A.Lutje, V.Kennedy, D.Authié, E.Boulange, A.F.Logan-Henfrey, L.L.Gichuki, B.Gettinby, G.[Trypanosoma congolense: B-Lymphocyte responses differ between trypanotolerant and trypanosusceptible cattle]Trypanosoma congolense: B-Lymphocyte responses differ between trypanotolerant and trypanosusceptible cattle

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