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Two Ag-ELISAs, an IgG-specific antibody detection ELISA (IgG ELISA) and a card agglutination test (CATT) for the detection of Trypanosoma evansi infections in buffaloes in Indonesia, were compared. Diagnostic sensitivity estimates were obtained by testing sera from 139 Indonesian buffaloes which ...


Davison, H.C.Thrusfield, M.V.Muharsini, S.Husein, A.Partoutomo, S.Rae, P.F.Masake, R.Luckins, A.G.[Evaluation of antigen detection and antibody detection tests for Trypanosoma evansi infections of buffaloes in Indonesia]Evaluation of antigen detection and antibody detection tests for Trypanosoma evansi infections of buffaloes in Indonesia

The indirect enzyme-linked immunosorbent assay (ELISA) for the detection of anti-trypanosomal antibodies in bovine serum was adapted for use with dried blood spots on filter paper. Aborbance (450 nm) results for samples were expressed as percent positivity, i.e., percentage of the median absorban...


Hopkins, J.S.Chitambo, H.Machila, N.Luckins, A.G.Rae, P.F.Bossche, P. van denEisler, M.C.[Adaptation and validation of antibody-ELISA using dried blood spots on filter paper for epidemiological surveys of tsetse-transmitted trypanosomosis in cattle]Adaptation and validation of antibody-ELISA using dried blood spots on filter paper for epidemiological surveys of tsetse-transmitted trypanosomosis in cattle

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